太平洋戦争時期におけるアメリカの対中イメージの変化

指導教員: 埼玉大学教養学部教養学科ヨーロッパ文化・アメリカ研究講座教授 有賀夏紀textapplication/pdfthesi

交わりの拡張と創造性の縮小 : ミルトンの四離婚論をめぐる諸原理について

2004-11-02departmental bulletin pape

Development of cross-curriculum for the elementary school teacher training : collaborative practice with Japanese language and science

text紀要論文/Departmental Bulletin Paperdepartmental bulletin pape

Utilization of ICT in Physical Education Classes ? Using a Heart Rate Sensor and Tablet ?

In this report, we introduce an example of a lesson practice where a small heart rate sensor and tablet are utilized. In a simulated physical education class for high school students, we conducted a shuttle run test with the aim of discerning exercise intensity and physical burden. Runners were fitted with a heartbeat sensor, with their heart rates monitored in real time on the tablet. A projector was used to project these data on a screen, helping us to visualize heart rate changes. In addition to showing an interest in the relationship between running speed and physiological burden, students who participated in the class also demonstrated curiosity with regard to a variety of other facets of the research, including the measuring instruments themselves and data analysis using PCs.departmental bulletin pape

Oligopeptide Transporter-1 is Associated with Fluorescence Intensity of 5-Aminolevulinic Acid-Based Photodynamic Diagnosis in Pancreatic Cancer Cells

[Background] The 5-aminolevulinic acid (ALA)-based photodynamic diagnosis is based on the accumulation of photosensitizing protoporphyrin IX in the tumor after ALA administration. However, the mechanisms connecting exogenous ALA and tumor fluorescence in pancreatic cancer remain unclear. We aimed to elucidate the mechanism underlying the ALA-induced fluorescent. [Methods] Human pancreatic duct epithelial cells (hPDECs) and pancreatic cancer cell lines were used. The expressions of ALA-associated enzymes and membrane transporters in these cell lines were investigated. ALA-induced fluorescence was also investigated. [Results] The expression of oligopeptide transporter-1 (PEPT-1), through which ALA is absorbed, was significantly higher in AsPC-1 cells and lower in MIA PaCa-2 cells than in hPDECs. AsPC-1 cells showed rapid and intense fluorescence after ALA administration, and that was attenuated by PEPT-1 inhibition. ALA-induced fluorescence was not sufficiently strong in MIA PaCa-2 cells to distinguish the cells from hPDECs. [Conclusion] We revealed the association of PEPT-1 with ALA-induced fluorescence. Cancers expressing PEPT-1 could be easily distinguished by this technique from normal cells. These findings help develop novel diagnostic modalities for pancreatic cancer.journal articl

Measurement of branching fractions and charge asymmetries for two-body B meson decays with charmonium

journal articl

ガゾウチュウ ノ オブジェクト オ リヨウシタ データベース ニ オケル ガゾウ ケンサク シュホウ

奈良先端科学技術大学院大学修士(工学)master thesi

Stability of lipase displayed on the surface of GM730/pJHC13 cells (■) and free form lipase (▲) during incubation at 37°C in a two-phase water-isooctane reaction system

<p><b>Copyright information:</b></p><p>Taken from "Display of a thermostable lipase on the surface of a solvent-resistant bacterium, GM730, and its applications in whole-cell biocatalysis"</p><p>BMC Biotechnology 2006;6():23-23.</p><p>Published online 19 Apr 2006</p><p>PMCID:PMC1459859.</p><p>Copyright © 2006 Jung et al; licensee BioMed Central Ltd.</p> Residual lipase activities were measured spectrophotometrically with pNPP as a substrate and calculated by assuming the initial activity was 100%

Ultrasensitive ImmunoMag-CRISPR Lateral Flow Assay for Point-of-Care Testing of Urinary Biomarkers

Rapid, accurate, and noninvasive detection of biomarkers in saliva, urine, or nasal fluid is essential for the identification, early diagnosis, and monitoring of cancer, organ failure, transplant rejection, vascular diseases, autoimmune disorders, and infectious diseases. We report the development of an Immuno-CRISPR-based lateral flow assay (LFA) using antibody-DNA barcode complexes with magnetic enrichment of the target urinary biomarkers CXCL9 and CXCL10 for naked eye detection (ImmunoMag-CRISPR LFA). An intermediate approach involving a magnetic bead-based Immuno-CRISPR assay (ImmunoMag-CRISPR) resulted in a limit of detection (LOD) of 0.6 pg/mL for CXCL9. This value surpasses the detection limits achieved by previously reported assays. The highly sensitive detection method was then re-engineered into an LFA format with an LOD of 18 pg/mL for CXCL9, thereby enabling noninvasive early detection of acute kidney transplant rejection. The ImmunoMag-CRISPR LFA was tested on 42 clinical urine samples from kidney transplant recipients, and the assay could determine 11 positive and 31 negative urinary samples through a simple visual comparison of the test line and the control line of the LFA strip. The LFA system was then expanded to quantify the CXCL9 and CXCL10 levels in clinical urine samples from images. This approach has the potential to be extended to a wide range of point-of-care tests for highly sensitive biomarker detection

Synthesis of triacylglycerol by lipase displayed on the GM730/pJHC13 cell surfaces in the isooctane solvent reaction system at 37°C

<p><b>Copyright information:</b></p><p>Taken from "Display of a thermostable lipase on the surface of a solvent-resistant bacterium, GM730, and its applications in whole-cell biocatalysis"</p><p>BMC Biotechnology 2006;6():23-23.</p><p>Published online 19 Apr 2006</p><p>PMCID:PMC1459859.</p><p>Copyright © 2006 Jung et al; licensee BioMed Central Ltd.</p> Conversion yield during incubation is shown