16 research outputs found
The nucleoporin ELYS/Mel28 governs formation of nuclear envelope (NE) subdomains in post-mitosis
学位記号番号 : 博理工甲第857号博士の専攻分野の名称 : 博士(理学)
学位授与年月日 : 平成24年3月22日textapplication/pdfthesi
Aerial photography experiment by fixed wing and vertical take-off / landing type drone FUTVTOL-1
An aerial photography experiment by using newly developed drone FUTVTOL-1 was performed around Awara campus at both day and night time zones on March 8, 2019. FUTVTOL-1 is a fixed wing type drone with the vertical take-off and landing function. Therefore, FUTVTOL-1 can fly at high speed for long time moreover does not require any other devices for take-off and landing. The many images obtained by onboarded low cost Raspberry Pi Camera and flight record clearly show FUTVTOL-1 is useful for wide area observation with high ground resolution. We carried out correlation analysis between the image taken by FUTVTOL-1 and NDVI image by Sentinel-2, a pair of large satellites operated by ESA. The result shows the effective and continuous measurement less affected by weather can be possible by the combination FUTVTOL-1 data and satellite data. In addition, night time images show the possibility FUTVTOL-1 can be used for monitor outdoor artificial lighting.departmental bulletin pape
Detection of Disease-specific Fusion Genes of Soft Tissue Tumors Using Formalin-fixed Paraffin-embedded Tissues; Its Diagnostic Usefulness and Factors Affecting the Detection Rates
[Background] Recent rapid advances in molecular biology have led the discovery of disease-specific novel fusion genes in a variety of soft tissue tumors. In this study, we attempted to detect these fusion genes using formalin-fixed paraffin-embedded (FFPE) tumor tissues and investigated their clinical utility and factors that affect the results of examination. [Methods] Reverse transcription polymerase chain reaction for the detection of tumor-specific fusion genes was performed using 41 FFPE tumor samples obtained from 37 patients representing nine histological types of soft tissue tumors that were diagnosed from 2006 to 2017 in our laboratory. [Results] Fusion genes in 19 (51.3%) out of 37 cases were detected successfully. Relatively high detection rates were observed in synovial sarcomas (100%, 4/4) and alveolar rhabdomyosarcomas (75%, 3/4). The detection rates of fusion genes were inversely correlated with the storage period of FFPE blocks. Decalcification by Plank-Rychlo solution significantly affected detection rates of the internal control gene (P = 0.0038). In contrast, there was no significant difference in detection rates between primary and metastatic lesion, or biopsy and resection material, or presence and absence of treatment history. [Conclusion] In certain histological types, detection of disease-specific fusion genes of soft tissue tumors using FFPE tissues showed high sensitivity and thus had diagnostic utility. However, due to the diversity of fusion patterns and the low-quality of nucleic acid, the detection rate as a whole was sluggish and required further improvement. For factors affecting the detection results, our results suggested that it was impossible to detect fusion genes by decalcified FFPE tissues, but it may be not necessary to consider factors such as the type of specimen (biopsy or resection) and treatment history of the patients when selecting the FFPE tissues.journal articl
Behavior of Radiocesium in Forest and Trees(Ⅴ) - Heartwood property and behavior of 137Cs in sugi wood -
text紀要論文 / Departmental Bulletin Paperdepartmental bulletin pape
Analysis of mRNA Levels in Biopsies of Epididymal Fat Depots Taken from Mice 7 wk of Age prior to Feeding a High-Fat Diet Suggests That Changes in Gene Expression Exist before Mice Are Exposed to an Obesogenic Environment
<p>Mean adiposity (FM/LM) at 16 wk of age for the mice with the highest and lowest 20% expression of SFRP5 (A), MEST (B), and BMP3 (C) at 7 wk of age. SFRP5 and MEST were assayed by qRT-PCR and BMP3 by a TaqMan® Low Density Array. Each bar represents the mean FM/LM ± SEM for 12 mice. (D) Induction of SFRP5 and MEST mRNA levels after being fed a high-fat diet at 8 wk of age for 1 wk.</p
Anomalous power and spectrum dependence of terahertz radiation from femtosecond-laser-irradiated indium arsenide in high magnetic fields up to 14 T
We report on the terahertz radiation from femtosecond-laser-irradiated indium arsenide in high magnetic fields up to 14 T. It is found that the radiation power exhibits anomalous magnetic-field dependence, including saturation, decrease, and recovery up to 14 T. Moreover, the radiation spectrum possesses a clear periodic structure over 6 T
Coordinated Gene Expression
<p>Regression analyses of SFRP5, MEST, and Naked mRNA levels in RNA isolated from high and low gainer mice in inguinal fat depots suggest that regulation of this subset of genes share a common mechanism related to the degree of adiposity. Twenty mice were present in each group.</p
