ロープづりされた荷の振動(機械力学,計測,自動制御)

Authors are aiming at system construction to make the load descent to the bottom of the hole and to make it turn for decided direction. The load is hung with two ropes, and the direction is done by rotating the top part where the rope is installed. In that case, the situation that a rope each other intersects along with the rotation of the top part. Therefore, the gyration is caused on the load. It is though that such behavior is the same one caused by the winch. However, most of the report for the winch is the one that the optimum conditions when the swing is not caused, and it transports it to the load in the straight line are induced. The rope for hanging temporarily causes intersection mutually, and when an irregular movement is caused, it is not analyzed. In this report, the gyration of the load for this case was analyzed.textapplication/pdfjournal articl

多人数用透析装置における熱湯クエン酸消毒の有効性

Article長野県透析研究会誌 33(1): 143-146(2010)journal articl

Search for Invisible Decay of the Υ(1S)

We report results of a search for the invisible decay of the Υ(1S) via the Υ(3S)→π+π-Υ(1S) transition using a data sample of 2.9 fb^{-1} at the Υ(3S) resonance. The data were collected with the Belle detector at the KEKB asymmetric-energy e+e- collider. No signal is found, and an upper limit for the branching fraction at the 90% confidence level is determined to be B(Υ(1S)→invisible)＜2.5×10^{-3}.journal articl

Evidence for D0-0 Mixing

journal articl

ヒカリ ト カッセイ サンソ オ リヨウシタ イデンシ フカッセイカホウ ノ カイハツ

奈良先端科学技術大学院大学修士(工学)master thesi

Different Wnt proteins display distinct activities.

<p>The graphs on the left depict activity in real-time bioluminescence monitoring assays. Values from the 24 hour timepoint were extracted for statistical analysis and are presented in the charts on the right.A–C) 293A-STF cells and CHO cells were seeded together in a 96 well plate in the presence of Dox, the total number of CHO cells per well was kept constant.</p

Use of a Molecular Genetic Platform Technology to Produce Human Wnt Proteins Reveals Distinct Local and Distal Signaling Abilities

<div><p>Functional and mechanistic studies of Wnt signaling have been severely hindered by the inaccessibility of bioactive proteins. To overcome this long-standing barrier, we engineered and characterized a panel of Chinese hamster ovary (CHO) cell lines with inducible transgenes encoding tagged and un-tagged human <i>WNT1</i>, <i>WNT3A</i>, <i>WNT5A</i>, <i>WNT7A</i>, <i>WNT11</i>, <i>WNT16</i> or the soluble Wnt antagonist <i>Fzd8CRD</i>, all integrated into an identical genomic locus. Using a quantitative real-time bioluminescence assay, we show that cells expressing WNT1, 3A and 7A stimulate Wnt/beta-catenin reporter activity, while the other WNT expressing cell lines interfere with this activation. Additionally, in contrast to WNT3A, WNT1 only exhibits activity when cell-associated, and thus only signals to neighboring cells. The reporter assay also revealed a rapid decline of Wnt activity at 37°C, indicating that Wnt activity is highly labile. These engineered cell lines will reduce the cost of making and purifying Wnt proteins and serve as a continuous, reliable and regulatable source of Wnts to research laboratories around the world.</p> </div

Activity of iCHO-produced Wnt Proteins.

<p>The graphs on the left depict induction of SUPERTOPFLASH (STF) activity in real-time bioluminescence monitoring assays. Values from the 24 hour timepoint were extracted for statistical analysis and are presented in the charts on the right.A) CM was collected from iCHO cells grown in the presence of Dox and 100 µL was added to reporter cells (mouse L + STF) in a 96 well plate. The volume of CM was kept constant such that ‘FLAG-hWNT3A:Par’ contained 50 µL FLAG-hWNT3A CM and 50 µL CM from parental CHO cells. Thus, ‘FLAG-hWNT3A:FLAG-hWNT5A’ CM and ‘FLAG-hWNT3A:Par’ each have an equal amount of FLAG-hWNT3A CM. Arrow points to FLAG-hWNT1 in CM. B, D–G) 293A-STF cells and iCHO cells were seeded together in a 96 well plate in the presence of Dox. Accumulation of luciferase activity is delayed compared to CM, presumably because it takes some time for the iCHO cells to produce Wnt following Dox stimulation. The total number of iCHO cells per well was kept constant such that ‘FLAG-hWNT3A’ contained 100% FLAG-hWNT3A cells and ‘FLAG-hWNT3A:Par’ contained 50% FLAG-hWNT3A cells and 50% Parental CHO cells. C) Anti-WNT3A and anti-WNT5A western blots comparing the levels of tagged and untagged WNTs in cell lysates and conditioned media. Quantification is shown below, FLAG staining was normalized to non-specific bands.</p

WNT1 acts locally.

<p>A) iCHO cells expressing untagged hWNT1 or hWNT3A were co-cultured with 293A-STF cells at varying cell densities. The ratio of hWNT3A-induced STF activity to hWNT1-induced STF activity is shown for three timepoints. The rate of reporter induction by WNT3A is higher than WNT1 at low cell densities, but not at high cell densities. B) The BSTG cell line was generated to distinguish iCHO cells (uncolored) from 293 reporter cells (blue). iCHO cells were seeded at 1000 cells per 10 cm plate and allowed to form colonies for 4 days prior to the addition of BSTG cells. Dox was added to plates after 24 hours to induce Wnt expression. Images were taken 48 hours later. iCHO colonies were located based on cell morphology and confirmed by corresponding gaps in the blue channel. The BSTG cell line is not clonal and cells exhibit varying levels of blue fluorescence. Unprocessed GFP images taken with identical exposure settings are shown, additional unprocessed images are shown in Fig. S6. C) Flow cytometry analysis of the cells used in co-culture imaging experiments. The plots show GFP expression of 50,000 live BSTG cells. The bar graphs depict the number of GFP+ cells in the ‘P4’ gate (left) and the geometric mean GFP intensity of those cells (right).</p

奥日光の森林植生の50年間の変化 ー過去と現在の植生記録の比較ー

text紀要論文 / Departmental Bulletin Paperdepartmental bulletin pape