The Current Status of Digital Transformation (DX) in Japanese and Chinese Companies and Its Impact on Human Capital Acquisition and Development.

亜細亜大学修士（経営学）thesi

Observation of b→dγ and Determination of |Vtd/Vts|

journal articl

為兼の表現―「玉葉集」中の為兼歌をめぐって―

1986-03departmental bulletin pape

Canopy1, a positive feedback regulator of FGF signaling, controls progenitor cell clustering during Kupffer's vesicle organogenesis

The assembly of progenitor cells is a crucial step for organ formation during vertebrate development. Kupffer’s vesicle (KV) is a key organ required for the left-right asymmetric body plan in zebrafish, and is generated from a cluster of approximately 20 dorsal forerunner cells (DFCs). Although several genes are known to be involved in KV formation, how DFC clustering is regulated and how cluster formation then contributes to KV formation remain unclear. Here we show that positive feedback regulation of FGF signaling by Canopy1 (Cnpy1) controls DFC clustering without affecting DFC specification and DFC number. Cnpy1 positively regulates FGF signals within DFCs, which in turn promotes Cadherin1-mediated cell adhesion between adjacent DFCs to sustain cell cluster formation. When this FGF positive feedback loop is disrupted, the DFC cluster fails to form, eventually leading to KV malformation and defects in the establishment of laterality. Our results therefore uncover both a previously unidentified role of FGF signaling during vertebrate organogenesis and a regulatory mechanism underlying cell cluster formation, which is an indispensable step for formation of a functional KV and establishment of the left-right asymmetric body plan.journal articl

プラットホーム指向の歩行者WYSIWYASナビゲーションシステムの提案

本論文では，ポジショニングサブシステムのマイグレーションを考慮したプラットホーム指向の歩行者WYSIWYASナビゲーションシステムを提案している．提案システムでは，建物内や建物付近の屋外でシームレスに動作し，かつ，WYSIWYASナピゲーションの実現に必要な位置情報と方向情報を他の位置特定手法と協調することなく取得可能なポジショニングサブシステムとして，タイルカーペットを用いたM-CubITSと可視光通信を採用している．大型商業複合施設内で目的地の名称のみが分かっている状況での移動を想定した評価実験では，提案システムにおけるポジショニングサブシステムの位置特定成功率，処理時間，移動所要時間，ユーザビリティについて評価を行っている．その結果，提案システムを用いることで，固定の案内板や手持ちの地図などを用いるよりも短時間で確実に目的地に到達できることから，提案システムの歩行者ナピゲーションシステムとしての有効性を確認している．copyright©2012 IEICE http://www.ieice.org/jpn/index.htmltextapplication/pdfjournal articl

Mice bearing microscopic NB-1643 tumors were injected intravenously with 2 million HB1.F3.C1 cells pre-labeled with CM-DiI Red Cell Tracker.

<div><p>Normal and tumor-bearing organs were harvested, sectioned, and stained with DAPI. HB1.F3.C1 cells were not detected in normal (A) brain, (B) kidney, (C) heart, (D) intestine, or (E) skin tissue.</p> <p>Rare, single, HB1.F3.C1 cells (white arrows) were seen in (F) lung, (G) liver and (H) spleen.</p> <p>Scale bars: 200 µm (A–E), 100 µm (F–H).</p></div

Therapeutic efficacy of rCE/CPT-11 NDEPT.

<div><p>Mice (10/group) received one of the following: 1) 2 million HB1.F3.C1 cells transduced with AdCMVrCE (MOI = 20) encoding a secreted form of rCE; 2) CPT-11 (7.5 mg/kg) alone; 3) transduced HB1.F3.C1 cells and CPT-11 (7.5 mg/kg).</p> <p>Animals that received HB1.F3.C1/AdCMVrCE cells with CPT-11 survived significantly longer than mice receiving only HB1.F3.C1/AdCMVrCE cells (<i>P</i><0.0001) or only CPT-11 (<i>P</i><0.001), suggesting an enhanced tumor targeting and tumor cell-killing effect of CPT-11.</p></div

HB1.F3.C1 cells injected intravenously localize to micrometastatic human NB-1643 neuroblastoma tumors in the liver of a mouse.

<div><p>(A) Dissected liver from a representative animal with hepatic metastases; the animal was sacrificed two days following injection of HB1.F3.C1 cells into the tail vein.</p> <p>(B) Low- and (C) high-power magnification of a section of tumor-involved liver, stained with hematoxylin and eosin.</p> <p>Tumor cells appear dark purple (black arrows); normal tissue appears pink.</p> <p>(D) Liver section stained with an anti-human mitochondrial protein antibody and counterstained with hematoxylin.</p> <p>Tumor micrometastases stain dark brown (red arrows); normal liver tissue is purple.</p> <p>(E) Immunofluorescence microscopy of liver section.</p> <p>HB1.F3.C1 cells were CM-DiI-labeled prior to injection and are evident as red cells.</p> <p>The liver section was stained with DAPI; tumor foci are identified by areas of densely-packed DAPI-stained tumor cell nuclei.</p> <p>The red arrows show extravasated HB1.F3.C1 cells proximal to a hepatic vein (v).</p> <p>Inset is high magnification of a DiI-labeled HB1.F3.C1 cell within the tumor. (F–H) Liver section from a tumor-bearing animal that received CM-DiI-labeled HB1.F3.C1 cells was stained with FITC-conjugated human specific mitochondrial antibody.</p> <p>(F) Red CM-DiI-labeled HB1.F3.C1 cells.</p> <p>(G) The same section showing FITC-labeled (green) human tumor cells and HB1.F3.C1 cells.</p> <p>(H) Overlay of F (red CM-DiI HB1.F3.C1 cells) and G (green FITC HB1.F3.C1 and tumor cells).</p> <p>HB1.F3.C1 cells (orange/yellow cells indicated by white arrows) migrated to hepatic micrometastases (green cells) and infiltrated the tumor parenchyma in the proximity of a blood vessel (bv, white dotted lines).</p> <p>Scale bars: 1 cm (A), 2 mm (B), 500 µm (C), 200 µm (F, G), 100 µm (D, E, H), 10 µm (E inset).</p></div

HB1.F3.C1 cells transduced with an adenoviral multiplicity of infection of 5, 10, or 20 retain their tumor-tropism toward conditioned medium from SK-N-AS neuroblastoma cells.

<p>Bars represent the average number of migrated cells±SEM of triplicate wells in modified Boyden chamber assays.</p

HB1.F3.C1 cells injected intravenously localized to microscopic bone marrow disease.

<div><p>Concordant detection of v-<i>myc</i> (HB1.F3.C1 cells) by PCR and TH expression (neuroblastoma cells) by RT-PCR in bone marrow specimens.</p> <p>Bone marrow samples isolated from animals injected with HB1.F3.C1 cells were analyzed for the presence of v-<i>myc</i> (HB1.F3.C1 cells) or the expression of TH (NB-1643 cells).</p> <p>HB1.F3.C1 cells were present in the bone marrow only when tumor cells were also present.</p> <p>HB1.F3.C1 cells were not detected in the bone marrow of non-tumor-bearing animals.</p> <p>The positive controls (+) were DNA extracted from HB1.F3.C1 cells for v-<i>myc</i>, and RNA extracted from NB-1691 cells for TH.</p> <p>The negative controls (−) contained no DNA or RNA template, respectively.</p></div