Analysis on household water insecurity, health and thefeasibility of alternative techniques for sustainable water usein developing country settings

University of Yamanashi (山梨大学)博士(工学)doctoral thesi

Japanese University Students' Perspective on NEST & N-NEST

departmental bulletin pape

Observation of b→dγ and Determination of |Vtd/Vts|

journal articl

Essay on Ventilation of Mines

工部大学校種別:卒業論文thesi

マルティン・ハイデガーの政治思想（四・完） 『存在と時間』における「民族」の可能性

2007-12-25departmental bulletin pape

HMG Domain Containing SSRP1 Is Required for DNA Demethylation and Genomic Imprinting in Arabidopsis

In Arabidopsis, DEMETER (DME) DNA demethylase contributes to reprogramming of the epigenetic state of the genome in the central cell. However, other aspects of the active DNA demethylation processes remain elusive. Here we show that Arabidopsis SSRP1, known as an HMG domain-containing component of FACT histone chaperone, is required for DNA demethylation and for activation and repression of many parentally imprinted genes in the central cell. Although loss of DNA methylation releases silencing of the imprinted FWA-GFP, double ssrp1-3;met1-3 mutants surprisingly showed limited activation of maternal FWA-GFP in the central cell, and only became fully active after several nuclear divisions in the endosperm. This behavior was in contrast to the dme-1;met1 double mutant in which hypomethylation of FWA-GFP by met1 suppressed the DNA demethylation defect of dme-1. We propose that active DNA demethylation by DME requires SSRP1 function through a distinctly different process from direct DNA methylation control.journal articl

Analysis of osteoclastogenesis from CaP patients' PBMCs.

<p>TRAP positive multinucleated cells were identified as OCs and counted, in both patients and healthy controls cultures, (A). The OC number in bone metastatic patients was significantly higher than in non-bone metastatic patients, <i>p</i><0.004 and in healthy controls, <i>p</i><0.001 (B).</p

IL-7 expression by CaP.

<p>IL-7 serum levels in patients with/without bone metastases and in healthy controls were measured by ELISA. Bone metastatic (<i>p</i><0.01) and non-bone metastatic patients (<i>p</i><0.03) had significantly higher IL-7 serum levels compared to healthy controls (A). CaP and healthy tissues were analyzed by Real-Time PCR in order to quantify IL-7 gene expression. The IL-7 quantization was expressed as IL-7 on β-Actin (the control gene) plasmid copy number. The histogram showed comparable IL-7 expression levels in CaP and healthy tissues.</p

Frequency distribution of single point mutations occurring at codon 12 of K-RAS in morphologically not acceptable, acceptable and dataset mCRC-derived tumor specimens.

<p><i>NA</i> = Not acceptable samples</p><p><i>A</i> = Acceptable samples</p><p>Cosmic DB = Cosmic database of somatic mutations</p><p>Frequency distribution of single point mutations occurring at codon 12 of K-RAS in morphologically not acceptable, acceptable and dataset mCRC-derived tumor specimens.</p

Quantitative analysis of IL-7 expression in T and B cells.

<p>IL-7 expression was evaluated by RQ-PCR using 2^−ΔΔCt method. B cells derived from cancer patients expressed significantly higher levels of IL-7 compared to T cells. Both T and B cells from healthy controls expressed less IL-7 than patients. The control bar represents the negative control: IL-7 expression in H522 cell line. Data are means±SE of nine independent experiments.</p