Simultaneous calibration: a joint optimization approach for multiple Kinect and external cameras

No description supplie

AMPLIFICATION AND SUBSTANTIAL PURIFICATION OF CARDIOLIPIN SYNTHASE OF ESCHERICHIA-COLI

A simple, specific, and sensitive assay procedure for cardiolipin synthase of Escherichia coli has been developed. This measures the radioactivity of glycerol formed from phosphatidyl[2-3H]glycerol and is mainly based on the findings that 400 mM phosphate and 0.015% Triton X-100 markedly activate the enzyme. Cardiolipin synthase was amplified 760-fold upon induction with isopropyl β-D-thiogalactoside in cells harboring a pBR322 derivative in which the cls gene encoding this enzyme was preceded by the tac promoter. Under these conditions, cardiolipin content increased, membrane potential decreased, spheroplasts became fragile, cells lost viability, and inducer-resistant mutants appeared at a high frequency. The amplification enabled the isolation of an enzyme preparation with a specific activity approximately 10,000-times higher than that of wild-type whole cell lysate. This purification was simply achieved by extraction of the crude membrane fraction with Triton X-100 and a single phosphocellulose column chromatography. This preparation, together with the crude envelope fraction, was used to characterize the basic properties of E. coli cardiolipin synthase, some of which were utilized in setting up the assay conditions.textapplication/pdfjournal articl

Intergenerational transmission of maternal behavioral traits in mice: involvement of the gut microbiota

The matrilineal transmission of maternal behavior has been reported in several species. Studies, primarily on rats, have suggested the importance of postnatal experience and the involvement of epigenetic mechanisms in mediating these transmissions. This study aims to determine whether the matrilineal transmission of maternal behavior occurs in mice and whether the microbiota is involved. We first observed that early weaned (EW) female mice showed lower levels of maternal behavior, particularly licking/ grooming (LG) of their own pups, than normally weaned (NW) female mice. This difference in maternal behavioral traits was also observed in the second generation, even though all mice were weaned normally. In the subsequent cross-fostering experiment, the levels of LG were influenced by the nurturing mother but not the biological mother. Finally, we transplanted the fecal microbiota from EW or NW mice into germ-free (GF) mice raising pups. The maternal behaviors that the pups exhibited toward their own offspring after growth were analyzed, and the levels of LG in GF mice colonized with microbiota from EW mice were lower than those in GF mice colonized with microbiota from NW mice. These results clearly indicate that, among maternal behavioral traits, LG is intergenerationally transmitted in mice and suggest that the vertical transmission of microbiota is involved in this process. This study demonstrates the universality of the intergenerational transmission of maternal behavioral traits and provides new insights into the role of microbiota.journal articl

Observation of b→dγ and Determination of |Vtd/Vts|

journal articl

A Skeleton Design of Siemens-Martin Furnace（平炉計画）

東京帝国大学工科大学種別:卒業論文thesi

Substituent Effects on Ring Opening of 2-Furylcarbenes: An ab Initio Study

The ring-opening reactions of a series of substituted 2-furylcarbenes were investigated by G2(MP2,SVP) theory. The substitutents examined include CH3, SiH3, NH2, OH, F, Cl, Br, and CHCH2. The furan ring-opening reaction is predicted to proceed via a six-membered ring transition state, which was described by Herges as a coarctate transition state. The calculated activation barrier depends strongly on the nature of the substituent group, ranging from 8 to 83 kJ mol-1. Substituent effects on the carbene reactant and ring-opening transition state were analyzed using isodesmic reactions. Methyl and vinyl substituents stabilize the reactant and transition state to a similar extent. On the other hand, NH2, OH, F, Cl, and Br substituents raise the energy barrier due to the greater stabilizing effect on the reactant than the transition state. The predicted aptitude of ring opening in 2-furylcarbenes is in the order SiH3 > H > CHCH2 > CH3 > Br > Cl > F > NH2 > OH. This calculated trend is consistent with experimental observations. The amino-, hydroxyl-, and fluoro-substituted 2-furylcarbenes are predicted to be experimentally accessible species in the gas phase. The effects of substitution on furan oxygen of 2-furylcarbene are also reported

Urubilva-pratiharyaの偈頌の伝承形態

1979-03departmental bulletin pape

Comparative analysis of the experimental results of the three protocols.

Comparative analysis of the experimental results of the three protocols.</p

Statistically and Computationally Efficient Estimating Equations for Large Spatial Datasets

<div><p>For Gaussian process models, likelihood based methods are often difficult to use with large irregularly spaced spatial datasets, because exact calculations of the likelihood for <i>n</i> observations require <i>O</i>(<i>n</i>³) operations and <i>O</i>(<i>n</i>²) memory. Various approximation methods have been developed to address the computational difficulties. In this paper, we propose new unbiased estimating equations based on score equation approximations that are both computationally and statistically efficient. We replace the inverse covariance matrix that appears in the score equations by a sparse matrix to approximate the quadratic forms, then set the resulting quadratic forms equal to their expected values to obtain unbiased estimating equations. The sparse matrix is constructed by a sparse inverse Cholesky approach to approximate the inverse covariance matrix. The statistical efficiency of the resulting unbiased estimating equations are evaluated both in theory and by numerical studies. Our methods are applied to nearly 90,000 satellite-based measurements of water vapor levels over a region in the Southeast Pacific Ocean.</p></div

Five scenarios for |<i>x</i>₂−<i>x</i>₁|<|<i>y</i>₂−<i>y</i>₁|.

Five scenarios for |x2−x1|y2−y1|.</p