D.H. Lawrence's idea of the nature of man : in comparison with G.I. Gurdjieff's ideas

application/pdfdepartmental bulletin pape

deep nausea of disillusion カラ ノ カイユ : D.H.Lawrence The man who died シロン

application/pdfdepartmental bulletin pape

Observation of b→dγ and Determination of |Vtd/Vts|

journal articl

The Design of Gas Producer Plant in Connection with Open-Hearth Furnace Work（瓦斯製炉計画）

東京帝国大学工科大学種別:卒業論文thesi

文字の回転に対して頑健な漢字認識手法

application/pdf三重大学大学院工学研究科博士前期課程情報工学専攻33thesi

『寇変紀』の世界―李世熊と明末清初福建寧化県の地域社会―

1991-03-31departmental bulletin pape

Neurons derived from transplanted neural stem cells restore disrupted neuronal circuitry in a mouse model of spinal cord injury

The body's capacity to restore damaged neural networks in the injured CNS is severely limited. Although various treatment regimens can partially alleviate spinal cord injury (SCI), the mechanisms responsible for symptomatic improvement remain elusive. Here, using a mouse model of SCI, we have shown that transplantation of neural stem cells (NSCs) together with administration of valproic acid (VPA), a known antiepileptic and histone deacetylase inhibitor, dramatically enhanced the restoration of hind limb function. VPA treatment promoted the differentiation of transplanted NSCs into neurons rather than glial cells. Transsynaptic anterograde corticospinal tract tracing revealed that transplant-derived neurons reconstructed broken neuronal circuits, and electron microscopic analysis revealed that the transplant-derived neurons both received and sent synaptic connections to endogenous neurons. Ablation of the transplanted cells abolished the recovery of hind limb motor function, confirming that NSC transplantation directly contributed to restored motor function. These findings raise the possibility that epigenetic status in transplanted NSCs can be manipulated to provide effective treatment for SCI.journal articl

(A) Testicular cells, and sperm from cauda epididymis and vas deferens were ( ) or were not (-) treated with biotin or trypsin and then subjected to western blot analysis

ADAM2, known to be located at the sperm surface, was included as a control. TC, testicular (spermatogenic) cells; S, mature sperm from the epididymis and vas deferens. ADAM, a disintegrin and metalloprotease. (B) Sperm from the epididymis and vas deferens were fractionated into heads and tails. These fractions were subjected to western blot analysis. ADAM2 localized on sperm head and α-tubulin composing the axoneme in the sperm flagellum were used as control proteins.<p><b>Copyright information:</b></p><p>Taken from "Characterization of eight novel proteins with male germ cell-specific expression in mouse"</p><p>http://www.rbej.com/content/6/1/32</p><p>Reproductive biology and endocrinology : RB&E 2008;6():32-32.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2500023.</p><p></p

(A) Testicular germ cells were isolated from testis, boiled in 3% SDS with 5% β-mercaptoethanol, subjected to SDS-PAGE and blotted with antibodies

Two antibodies were used for the test of antibody specificity to each protein. The first one is a control antibody affinity-purified against GST (G) protein from each serum. The second one is a test antibody affinity-purified against each GST fusion protein (G-F) used for immunization and serum production. ADAM2, known to exhibit spermatogenic cell-specific expression, was included as a control. (B) GST or GST fusion (GST-F) proteins were added to a buffer containing the first antibodies during the incubation of immune blot analysis.<p><b>Copyright information:</b></p><p>Taken from "Characterization of eight novel proteins with male germ cell-specific expression in mouse"</p><p>http://www.rbej.com/content/6/1/32</p><p>Reproductive biology and endocrinology : RB&E 2008;6():32-32.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2500023.</p><p></p

(A) Acrosome reaction of sperm from the epididymis and vas deferens was induced by calcium ionophore A23187

Acrosome-intact and -reacted sperm were subjected to western blot anaylsis with the anti-Shsp1/Mm.87328 antibody. AI, acrosome-intact sperm; AR, acrosome-reacted sperm. (B) Sperm from the epididymis and vas deferens were treated with 1% Triton X-100 and urea with different concentrations (2, 3, 4 and 6 M). Soluble and insoluble fractions after centrifugation of the treated sperm were subjected to western blot anaylsis with the anti-Sfap1/Mm.386907 and anti-Sfap2/Mm.157049 antibodies. Sup, supernatant after centrifugation; Pt, pellet after centrifugation.<p><b>Copyright information:</b></p><p>Taken from "Characterization of eight novel proteins with male germ cell-specific expression in mouse"</p><p>http://www.rbej.com/content/6/1/32</p><p>Reproductive biology and endocrinology : RB&E 2008;6():32-32.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2500023.</p><p></p