Targeted sequencing of early colorectal tumors reveals that gene alterations accumulate with cancer progression

University of Yamanashi (山梨大学)博士(医学)医工博4甲第257号thesi

Negative feedback by IRE1β optimizes mucin production in goblet cells

In mammals, the prototypical endoplasmic reticulum (ER) stress sensor inositol-requiring enzyme 1 (IRE1) has diverged into two paralogs. IRE1α is broadly expressed and mediates the unconventional splicing of X-box binding protein 1 (XBP1) mRNA during ER stress. By contrast, IRE1β is expressed selectively in the digestive tract, and its function remains unclear. Here, we report that IRE1β plays a distinctive role in mucin-secreting goblet cells. In IRE1β-/- mice, aberrant mucin 2 (MUC2) accumulated in the ER of goblet cells, accompanied by ER distension and elevated ER stress signaling such as increased XBP1 mRNA splicing. In contrast, conditional IRE1α-/- mice showed no such ER distension but a marked decrease in spliced XBP1 mRNA. mRNA stability assay revealed that MUC2 mRNA was greatly stabilized in IRE1β-/- mice. These findings suggest that in goblet cells, IRE1β, but not IRE1α, promotes efficient protein folding and secretion in the ER by optimizing the level of mRNA encoding their major secretory product, MUC2.journal articl

Observation of b→dγ and Determination of |Vtd/Vts|

journal articl

久根銅山採鉱計画

東京帝国大学工科大学種別:卒業論文thesi

十五世紀江南デルタの済農倉をめぐる資料について

1994-03-31departmental bulletin pape

Additional file 9: of Profiling of testis-specific long noncoding RNAs in mice

Table S9. Testis-specific lncRNAs and mRNAs in three mouse germ cell-related cell lines (F9, GC-1 and GC-2). (XLSX 712 kb

＜研究ノート＞シラバスで公開された授業の方法・目的類型別に見た大学の成績評価の実態分析 : 横浜国立大学におけるケーススタディ

社会のボーダレス化・グローバル化、ICT 化などが急速に進展し、我が国においても国境を越えた人・物・サービスの移動が身近に感じられるようになった。即戦力を求める企業などを中心にグローバル化などに対応できる人材の育成が望まれている。また大学に対しても、これらの社会的ニーズに対応できる人材育成の要望が大きくなっている。しかしながら、我が国の大学教育はこれまで学問を中心に形成されてきており、こうした要望には必ずしも迅速かつ十分に対応出来ているとは言えない。そこで本研究では、大学における社会的ニーズに対応した人材育成の実現を図るための研究の端緒として「成績評価」に焦点を当て、公開されたシラバス情報を基に、授業の方法・目的類型別に見た大学の成績評価の実態を分析した。As the borderlessness/globalization of society and information-communication technology rapidly advanced, the movement of people, products and services beyond national borders have become a matter of common practice to us in Japan. The business enterprises seek work-ready personnel and desire human resources adaptable to these changes. Universities are also expected to develop human resources adaptable to these social needs. However, in Japan, university education has been centered on the enhancement of academic studies. It remains a long way from promptly and sufficiently adapting to these needs. In order to mark the beginning of the research on the realization of human resource development adaptable to the social needs, this research focuses on academic grades and analyze the actual evaluation on learning results with regards to respective teaching methods and objectives at a university by using the syllabi which are publicly available.departmental bulletin pape

Additional file 4: of Profiling of testis-specific long noncoding RNAs in mice

Table S4. The numbers and ratios of six types of testicular lncRNA. (XLSX 10 kb

(A) Testicular cells, and sperm from cauda epididymis and vas deferens were ( ) or were not (-) treated with biotin or trypsin and then subjected to western blot analysis

ADAM2, known to be located at the sperm surface, was included as a control. TC, testicular (spermatogenic) cells; S, mature sperm from the epididymis and vas deferens. ADAM, a disintegrin and metalloprotease. (B) Sperm from the epididymis and vas deferens were fractionated into heads and tails. These fractions were subjected to western blot analysis. ADAM2 localized on sperm head and α-tubulin composing the axoneme in the sperm flagellum were used as control proteins.<p><b>Copyright information:</b></p><p>Taken from "Characterization of eight novel proteins with male germ cell-specific expression in mouse"</p><p>http://www.rbej.com/content/6/1/32</p><p>Reproductive biology and endocrinology : RB&E 2008;6():32-32.</p><p>Published online 24 Jul 2008</p><p>PMCID:PMC2500023.</p><p></p

Additional file 3: of Profiling of testis-specific long noncoding RNAs in mice

Table S3. The numbers and ratios of testicular lncRNAs and mRNAs on each mouse chromosome. (XLSX 11 kb