Usefulness of combining computed tomography and air sac fluid examination to rule out aspergillosis: case study in two gentoo penguins (Pygoscelis papua) with respiratory clinical signs

Pulmonary aspergillosis occurring in captive penguins living in zoos and aquariums is a fatal disease owing to its high mortality rate. On conducting computed tomography, two gentoo penguins (Pygoscelis papua) with respiratory clinical signs that were housed at an aquarium in Hokkaido, Japan were found to have a certain amount of fluid in their air sacs. This fluid was collected and cultured for bacteria and fungi, the results of which both came back negative. These results enabled us to rule out bacterial infection or aspergillosis and supported our decision not to administer antibacterial and antifungal drugs. Overall, the combination of computed tomography and air sac fluid examination was useful for ruling out bacterial infection or aspergillosis in penguins with respiratory clinical signs.journal articl

Improved Search for νμ→νe Oscillation in a Long-Baseline Accelerator Experiment

journal articl

MAB4-induced auxin sink generates local auxin gradients in Arabidopsis organ formation

In Arabidopsis, leaves and flowers form cyclically in the shoot meristem periphery and are triggered by local accumulations of the plant hormone auxin. Auxin maxima are established by the auxin efflux carrier PIN-FORMED1 (PIN1). During organ formation, two distinct types of PIN1 polarization occur. First, convergence of PIN1 polarity in the surface of the meristem creates local auxin peaks. Second, basipetal PIN1 polarization causes auxin to move away from the surface in the middle of an incipient organ primordium, thought to contribute to vascular formation. Several mathematical models have been developed in attempts to explain the PIN1 localization pattern. However, the molecular mechanisms that control these dynamic changes are unknown. Here, we show that loss-of-function in the MACCHI-BOU 4 (MAB4) family genes, which encode NONPHOTOTROPIC HYPOCOTYL 3-like proteins and regulate PIN endocytosis, cause deletion of basipetal PIN1 polarization, resulting in extensive auxin accumulation all over the meristem surface from lack of a sink for auxin. These results indicate that the MAB4 family genes establish inward auxin transport from the L1 surface of incipient organ primordia by basipetal PIN1 polarization, and that this behavior is essential for the progression of organ development. Furthermore, the expression of the MAB4 family genes depends on auxin response. Our results define two distinct molecular mechanisms for PIN1 polarization during organ development and indicate that an auxin response triggers the switching between these two mechanisms.revised versionjournal articl

後志鉱山製煉計画

東京帝国大学工科大学種別:卒業論文thesi

イミノマロネートへのタンデム付加反応による四級炭素構築と光学活性α-アミノ酸誘導体合成への応用

application/pdf三重大学大学院工学研究科博士前期課程分子素材工学専攻147thesi

舗床モザイクをめぐる試論（第一部―上―）

1974-03-31departmental bulletin pape

直流比抵抗法を用いたRC構造物の内部推定に関する基礎的研究

現在，鉄筋腐食状況を推定する非破壊検査法は，鉄筋端部をはつり出す一部破壊作業が必要である．そのため，電気探査法の一種である直流比抵抗法を用いて，鉄筋のはつり作業を行うことなくRC構造物の鉄筋腐食状況などを非破壊で推定する基礎的研究を実施した．実験では，健全鉄筋，腐食鉄筋，円形空洞の直径をパラメータとして，これらが直流比抵抗法で測定できるかの検討を行い，その実験結果から，鉄筋の有無，鉄筋腐食状況，空洞の有無を推定可能であることが明らかになった．解析では，三次元有限要素法の電界プログラムを開発し，数値シミュレーションを行って解析手法と解析コードの妥当性を検証すると共に，上記の実験結果と比較検討を行った．その結果，開発した電界プログラムは，RC構造物の内部推定に有効であることも明らかとなった．textapplication/pdfjournal articl

Additional file 1: Figure S1. of Pathological tau deposition in Motor Neurone Disease and frontotemporal lobar degeneration associated with TDP-43 proteinopathy

α-synuclein (a-c) pathology in cingulate gyrus (a), CA2 region of hippocampus (b) and substantia nigra (c) and TDP-43 pathology in anterior horn cells of the spinal cord (d-f), with fine, particulate accumulations of TDP-43 (d,e) or skein-like structures (e,f) being present in affected cells in which the nucleus has been ‘cleared’ of its normal immunoreactivity. Immunoperoxidase, x400 microscope magnification. (DOCX 6504 kb

Additional file 2: of Pathological tau deposition in Motor Neurone Disease and frontotemporal lobar degeneration associated with TDP-43 proteinopathy

File S1. Clinical histories and neuropathological findings in patients #35 and 41. (DOCX 21 kb

Analysis of IFT74 as a candidate gene for chromosome 9p-linked ALS-FTD.

BACKGROUND: A new locus for amyotrophic lateral sclerosis--frontotemporal dementia (ALS-FTD) has recently been ascribed to chromosome 9p. METHODS: We identified chromosome 9p segregating haplotypes within two families with ALS-FTD (F476 and F2) and undertook mutational screening of candidate genes within this locus. RESULTS: Candidate gene sequencing at this locus revealed the presence of a disease segregating stop mutation (Q342X) in the intraflagellar transport 74 (IFT74) gene in family 476 (F476), but no mutation was detected within IFT74 in family 2 (F2). While neither family was sufficiently informative to definitively implicate or exclude IFT74 mutations as a cause of chromosome 9-linked ALS-FTD, the nature of the mutation observed within F476 (predicted to truncate the protein by 258 amino acids) led us to sequence the open reading frame of this gene in a large number of ALS and FTD cases (n = 420). An additional sequence variant (G58D) was found in a case of sporadic semantic dementia. I55L sequence variants were found in three other unrelated affected individuals, but this was also found in a single individual among 800 Human Diversity Gene Panel samples. CONCLUSION: Confirmation of the pathogenicity of IFT74 sequence variants will require screening of other chromosome 9p-linked families.</p