External quality control survey on identification of microorganisms using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Abstract: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry（ MALDI-TOF MS） is a bacterial typing tool that was approved as a medical device in 2011. However, external accuracy control examination of bacterial typing using mass spectrometry is still only performed on a small scale. In this study, E. faecium and S. maltophilia were selected and tested according to established procedures using Score Values at 228 institutions. The Score Values for MALDI Biotyper were 2.43±0.08 for E. faecium and 2.38±0.08 for S. maltophilia; and those for VITEK MS/PRIME were 99.9±0.0 for E. faecium and S. maltophilia. These results suggest that it is useful to evaluate external accuracy control with Score Values using the procedures we have developed.journal articl

Structural basis of Sec-independent membrane protein insertion by YidC

Newly synthesized membrane proteins must be accurately inserted into the membrane, folded and assembled for proper functioning. The protein YidC inserts its substrates into the membrane, thereby facilitating membrane protein assembly in bacteria; the homologous proteins Oxa1 and Alb3 have the same function in mitochondria and chloroplasts, respectively1, 2. In the bacterial cytoplasmic membrane, YidC functions as an independent insertase and a membrane chaperone in cooperation with the translocon SecYEG3, 4, 5. Here we present the crystal structure of YidC from Bacillus halodurans, at 2.4 A resolution. The structure reveals a novel fold, in which five conserved transmembrane helices form a positively charged hydrophilic groove that is open towards both the lipid bilayer and the cytoplasm but closed on the extracellular side. Structure-based in vivo analyses reveal that a conserved arginine residue in the groove is important for the insertion of membrane proteins by YidC. We propose an insertion mechanism for single-spanning membrane proteins, in which the hydrophilic environment generated by the groove recruits the extracellular regions of substrates into the low-dielectric environment of the membrane.journal articl

Improved Search for νμ→νe Oscillation in a Long-Baseline Accelerator Experiment

journal articl

PBS-シラン処理HAp繊維複合材に関する実験的研究

application/pdf三重大学大学院工学研究科博士前期課程機械工学専攻63thesi

伊田炭坑竪坑計画

東京帝国大学工科大学種別:卒業論文thesi

護摩修法と火炉に関する一考察

1993-03departmental bulletin pape

Spectral responsivity measurement of radiation detectors by using optical fiber beamsplitters

The spectral responsivity measurement of radiation detectors by using optical fiber bundle beamsplitters is carried out. In this measurement, the optical fiber beamsplitter is set between a monochromator exit slit and two (standard and test) detectors. The bundle devides exit monochromatic radiation flux into two beams for standard and test detectors and also improves irradiance uniformity. The spectral responsivities of several Si photodiodes are measured by two different types of optical fiber bundle beamspliters and are compared with those by a conventional precise replacement method (replacement method). The results are as follows;(1) There is no essential difference between the results of our method and replacement one. (2) On our method, the standard and test detectores are measured at the same time (synchronous comparison method), so the intensity stability of a radiation source becomes less important. (3) The measurement period by use of the optical fiber beamsplitter becomes less half of that of replacement method. (4) The results of measurements are not influenced by the types of fiber bundles which have considerably different material, dimension and the number of single element fiber in our case.rights: 社団法人照明学会 rights: 本文データは学協会の許諾に基づきCiNiiから複製したものである relation: IsVersionOf: http://ci.nii.ac.jp/naid/110001144373/textapplication/pdfjournal articl

Analysis of IFT74 as a candidate gene for chromosome 9p-linked ALS-FTD.

BACKGROUND: A new locus for amyotrophic lateral sclerosis--frontotemporal dementia (ALS-FTD) has recently been ascribed to chromosome 9p. METHODS: We identified chromosome 9p segregating haplotypes within two families with ALS-FTD (F476 and F2) and undertook mutational screening of candidate genes within this locus. RESULTS: Candidate gene sequencing at this locus revealed the presence of a disease segregating stop mutation (Q342X) in the intraflagellar transport 74 (IFT74) gene in family 476 (F476), but no mutation was detected within IFT74 in family 2 (F2). While neither family was sufficiently informative to definitively implicate or exclude IFT74 mutations as a cause of chromosome 9-linked ALS-FTD, the nature of the mutation observed within F476 (predicted to truncate the protein by 258 amino acids) led us to sequence the open reading frame of this gene in a large number of ALS and FTD cases (n = 420). An additional sequence variant (G58D) was found in a case of sporadic semantic dementia. I55L sequence variants were found in three other unrelated affected individuals, but this was also found in a single individual among 800 Human Diversity Gene Panel samples. CONCLUSION: Confirmation of the pathogenicity of IFT74 sequence variants will require screening of other chromosome 9p-linked families.</p