The etherase system of Novosphingobium sp. MBES04 functions as a sensor of lignin fragments through phenylpropanone production to induce specific transcriptional responses

The MBES04 strain of Novosphingobium accumulates phenylpropanone monomers as end-products of the etherase system, which specifically and reductively cleaves the β-O-4 ether bond (a major bond in lignin molecules). However, it does not utilise phenylpropanone monomers as an energy source. Here, we studied the response to the lignin-related perturbation to clarify the physiological significance of its etherase system. Transcriptome analysis revealed two gene clusters, each consisting of four tandemly linked genes, specifically induced by a lignin preparation extracted from hardwood (Eucalyptus globulus) and a β-O-4-type lignin model biaryl compound, but not by vanillin. The most strongly induced gene was a 2,4'-dihydroxyacetophenone dioxygenase-like protein, which leads to energy production through oxidative degradation. The other cluster was related to multidrug resistance. The former cluster was transcriptionally regulated by a common promoter, where a phenylpropanone monomer acted as one of the effectors responsible for gene induction. These results indicate that the physiological significance of the etherase system of the strain lies in its function as a sensor for lignin fragments. This may be a survival strategy to detect nutrients and gain tolerance to recalcitrant toxic compounds, while the strain preferentially utilises easily degradable aromatic compounds with lower energy demands for catabolism.査読ありjournal articl

Effect of spin efficiency on pitching ability of fastball.

The purpose of this study was to clarify the influence of spin efficiency on pitching ability among high school baseball players. As a result, it was revealed a correlation between vertical break and spin efficiency in fastballs, highlighting their significance in generating effective pitches. Furthermore, no relationship was found between strike rates and spin efficiency, suggesting diverse factors affecting pitch control. Given that higher spin efficiency correlates with reduced gyro degree, conscious consideration of the spin axis in fastball will lead to pitches with more extension. Consequently, understanding the role of spin axis and spin efficiency in pitching may contribute to improve in pitching ability

Improved Search for νμ→νe Oscillation in a Long-Baseline Accelerator Experiment

journal articl

＜論文＞ギアチェンジ期におけるがん患者の役割期待に関する研究 : 病棟看護でみる役割理論からの概念枠組みの構築

本研究の目的は、ギアチェンジ（治療目的を治癒以外に方向転換し症状緩和を主とした治療の選択に変更していく時期）を告げられ意思決定する前のがん患者と、意思決定したがん患者の役割にはどのような変化が起きているのかを明らかにすることである。入院中の患者はどのような役割があり、他者からどのような役割を期待されているのか、「看護師から患者への役割期待」、「医師から患者への役割期待」、「家族から患者への役割期待」「病院から患者への役割期待」の4 つに分類して文献検討を行った。その結果、ギアチェンジ期におけるがん患者の役割期待は、ギアチェンジポイントを境に看護師・家族・医師の役割期待は変化すると捉え、ギアチェンジ期にあるがん患者の役割期待と病院からの役割期待を概念枠組みの図にまとめた。ギアチェンジ前は看護師、医師、家族はがん患者に対して、「積極的に病気と闘ってくださいという期待」があり、治療を行うが積極的治療にも限界が生じる。その瞬間が、ギアチェンジポントである。ギアチェンジ後、「症状緩和を最優先にしてくださいという期待」へと変化する。その一方で、病院のルールを守ってくださいという「病院からの患者への役割期待」に変化は起きていないことを導いた。これらのことから、看護師はがん患者の役割を知ることで、そのがん患者に合った接し方や看護の方法を工夫することができると示唆された。The purpose of this study is to clarify how the role of a cancer patient has been changing before and after the patient makes a decision toward the changing gear. The changing gear which is the period shifting the therapeutic purpose from cancer reduction to palliative care focusing on symptom reduction. In order to identify the patient’s roles and the patient’s roles expected from others during hospitalization, we examined the related documents by classifying the roles into four categories. The categories include “the roles expected from the nurse to the patient,” “the roles expected from the doctor to the patient,” “the roles expected from the family to the patient,” and “the roles expected from the hospital to the patient.” As a result, for the roles of cancer patients in the changing gear period, the expectation from the nurses, the family, and the doctors have changed before and after the changing gear point. In addition, we showed the summary of the expected roles of cancer patients in the changing gear period and the roles expected from the hospital in a figure of conceptual framework. Before the changing gear , the nurses, the doctors, and the family have the “expectation to fight thedisease actively” for the patient. Although the treatment to cure the cancer is performed, the treatment also has the limit. That moment is a changing gear point.After the changing gear, the patient has been shifted to the “expectation to put the first priority on the symptom relief.” On the other hand, we found that “the role expected from the hospital to the patient” that is to follow the hospital rules has not changed through the changing gear. From these findings, it was suggested that the nurses can improve their attitude and the nursing methods corresponding to the individual cancer patient by understanding the roles of cancer patients.departmental bulletin pape

超精密ダイヤモンド切削加工技術

rights: 社団法人日本機械学会 rights: 本文データは学協会の許諾に基づきCiNiiから複製したものである relation: IsVersionOf: http://ci.nii.ac.jp/naid/110002473895/textapplication/pdfjournal articl

東山鉱山採鉱計画

東京帝国大学工科大学種別:卒業論文thesi

濃度こう配特徴を用いた経年変化を含む顔の認識と年齢推定

application/pdf三重大学大学院工学研究科博士前期課程情報工学専攻41thesi

viridula

Nassella viridula (Trinius) Barkworthgreen needlegrassstipe verteStipa viridulatributary of Milk River 2.1 km SSW of Coffin BridgeDry Mixed Grassland: prairie incised river valley - dry short grass meadow and rock outcrops3275 fee

徂徠『論語徴』について（二）

1978-03departmental bulletin pape

Crystal structure of Escherichia coli YidC, a membrane protein chaperone and insertase

Bacterial YidC, an evolutionally conserved membrane protein, functions as a membrane protein chaperone in cooperation with the Sec translocon and as an independent insertase for membrane proteins. In Gram-negative bacteria, the transmembrane and periplasmic regions of YidC interact with the Sec proteins, forming a multi-protein complex for Sec-dependent membrane protein integration. Here, we report the crystal structure of full-length Escherichia coli YidC. The structure reveals that a hydrophilic groove, formed by five transmembrane helices, is a conserved structural feature of YidC, as compared to the previous YidC structure from Bacillus halodurans, which lacks a periplasmic domain. Structural mapping of the substrate- or Sec protein-contact sites suggested the importance of the groove for the YidC functions as a chaperone and an insertase, and provided structural insight into the multi-protein complex.journal articl