Early genetic changes identified in well-differentiated type early gastric carcinoma\r analyzed by deep sequencing

University of Yamanashi (山梨大学)博士(医学)医工博4甲第197号thesi

衛星データを用いた生駒山地における森林熱収支の季節変化の評価

Article農業気象. 1998, 54(2), p.143-154journal articl

Improved Search for νμ→νe Oscillation in a Long-Baseline Accelerator Experiment

journal articl

熱CVD法によるシリコン・カーボン複合構造の形成

application/pdf三重大学大学院工学研究科博士前期課程分子素材工学専攻65thesi

金瓜石鉱山計画

東京帝国大学工科大学種別:卒業論文thesi

実験室における動物行動研究の若干の問題―スンクス（Suncus murinus）の場合を例として―

1981-03departmental bulletin pape

光化学スモッグ被害原因物質の解明に関する研究

application/pdfpostprintSince the heavy damage due to photochemical smog took place in 1970, the study on its chemical origine has been conducted continuously. This report is one of which gas chromatographic technique was mainly applied. The three parts of the present research were environmental analysis, photochemical experiment of auto exhaust gas and that of aromatics. 1) Gas chromatographic and gas chromatograph-mass spectrometric analysis of air samples showed the existence of acrolein and methyl nitrate in urban atmosphere. Especially in smog two components were detected at high level. It was suggested that acrolein would cause eye irritation and methyl nitrate would disturb circulation of the blood. 2) A high content of toluene in the air was resulted from the auto exhaust from the burning of reforming gasoline of high octane value. Such carbonyl and nitrocompounds as acetone, acrolein, methyl nitrate, ethyl nitrate, nitromethane, peroxyacetyl nitrate, and nitroethane were found, from the result of photochemical reaction of the auto exhaust. Usually, methyl nitate was at highest concentration. The formation of large amounts of C_3 compound from smaller retio of unsaturated hydrocarbon like butenes except aromatics cannot be understood. 3) It was experimentally found that acrolein, acetone and methyl nitrate were formed through the photochemical reaction of toluene with nitrogen oxides. This fact intently suggested the cleavage of aromatic ring in toluene. In this process, the decreasing rate of toluene was so much as toluene seemed to be relate to smog formation.According to the relationships of these facts, the smog toxics would be made from the aromatics branched as toluene in auto exhaust gas.departmental bulletin pape

Calcium signalling mediates self-incompatibility response in the Brassicaceae

ARC1 is known to interact with, and is phosphorylated by, the kinase domain of SRK in Brassica napus9,10. ARC1 is a U-box protein with E3 ubiquitin ligase activity11, and interacts with Exo70A112, a putative component of the exocyst complex, which generally functions in polarized secretion13. These results suggested a model in which activated SRK phosphorylates ARC1, leading to the preclusion of as-yet unknown ‘compatibility factors’ secretion to the stigmatic surface and inhibiting pollen entrance14. However, the suppression of ARC1 expression results in incomplete breakdown of self-incompatibility in both B. napus and Arabidopsis lyrata10,14, and self-compatible Arabidopsis thaliana that lacks ARC1 acquires the self-incompatibility phenotype by introducing SRK and SP11/SCR genes15. Therefore, the extent ARC1 to which is involved in the signalling pathway downstream of SRK remains unclear8,16,17.In this study, we focused on investigating the cytoplasmic Ca2+ dynamics in stigma papilla cells during the self-incompatibility response. A previous study injected dyes to monitor Ca2+ dynamics in the self-incompatibility response18. By combining the in vivo imaging using genetically encoded [Ca2+]cyt probes and pharmacological approaches, we found that cytoplasmic Ca2+ drastically increases in the papilla cells after self-pollination, which can be efficiently blocked by the inhibitors of glutamate receptor channels that mediate the influx of extracellular Ca2+. Pretreatment of papilla cells with glutamate receptor channel inhibitor compromised the self-incompatibility response in vivo, whereas an artificial increase in [Ca2+]cyt in papilla cells induced arrest of pollen hydration to compatible pollen. The [Ca2+]cyt increase in papilla cells during the self-incompatibility response of GLR mutants was significantly reduced. Our results overall strongly suggest that the Ca2+ influx in papilla cells mediated by GLR is the key self-incompatibility response that leads to self-pollen rejection.Self-incompatibility in the Brassicaceae is controlled by multiple haplotypes encoding the pollen ligand (S-locus protein 11, SP11, also known as S-locus cysteine-rich protein, SCR) and its stigmatic receptor (S-receptor kinase, SRK). A haplotype-specific interaction between SP11/SCR and SRK triggers the self-incompatibility response that leads to self-pollen rejection, but the signalling pathway remains largely unknown. Here we show that Ca2+ influx into stigma papilla cells mediates self-incompatibility signalling. Using self-incompatible Arabidopsis thaliana expressing SP11/SCR and SRK, we found that self-pollination specifically induced an increase in cytoplasmic Ca2+ ([Ca2+]cyt) in papilla cells. Direct application of SP11/SCR to the papilla cell protoplasts induced Ca2+ increase, which was inhibited by D-(?)-2-amino-5-phosphonopentanoic acid (AP-5), a glutamate receptor channel blocker. An artificial increase in [Ca2+]cyt in papilla cells arrested wild-type (WT) pollen hydration. Treatment of papilla cells with AP-5 interfered with self-incompatibility, and Ca2+ increase on the self-incompatibility response was reduced in the glutamate receptor-like channel (GLR) gene mutants. These results suggest that Ca2+ influx mediated by GLR is the essential self-incompatibility response leading to self-pollen rejection.Flowering plants have developed self-incompatibility as a genetic system to prevent inbreeding and thus promote outcrossing. In many species, self-incompatibility is controlled by an S locus with multiple haplotypes1. Each S-haplotype encodes both male- and female-specificity determinants (S-determinants), and self/non-self-discrimination is accomplished by the S-haplotype-specific interaction between these S-determinants.In the Brassicaceae, the male and female S-determinants have been identified as SP11/SCR and SRK, respectively1. SP11/SCR is a polymorphic small peptide secreted from the anther tapetum that localizes to the pollen surface, whereas SRK is a polymorphic Ser/Thr receptor kinase that localizes to the plasma membrane of stigma papilla cells. SP11/SCR and SRK from each S-haplotype function respectively as a ligand and its cognate receptor. Upon self-pollination, the S-haplotype-specific interaction between SP11/SCR and SRK induces autophosphorylation of SRK, which is thought to trigger a signalling cascade in the papilla cells, resulting in the rejection of self-pollen2. Although self-pollination is known to evoke multiple physiological changes in the papilla cells, including disruption of actin bundles, fragmentation of vacuolar structure and modification of microtubules3,4, the signalling pathway downstream of SRK that leads to these processes remains largely unknown.Thus far, two candidate molecules, M-locus protein kinase (MLPK) and Arm-repeat containing 1 (ARC1), have been identified as the direct downstream effectors of SRK. MLPK was identified as a gene responsible for a self-compatibility mutation in Brassica rapa5, and encodes a membrane-anchored cytoplasmic protein kinase that interacts with SRK on the papilla cell membrane6. Recent studies have suggested that MLPK is also involved in intraspecies unilateral incompatibility of B. rapa7, but it remains unclear whether MLPK is required for self-incompatibility throughout the Brassicaceae.journal articl

視覚を用いたマイクロ液滴塗布システムの構築

An application technique for micro drops with diameter as small as 100 μm is desired for repairing the micro patterns of electronic circuits. In this paper we propose a micro drop application system that utilizes visual feedback control. A micro object handling robot, which is equipped with optical microscopes and a "task package", transfers micro drops one by one from the reservoir to the desired position on a substrate by means of a needle shaped tool, and applies them while controlling their diameter based on real time visual measurement. The experiment proved that the diameter of micro drops of metal paste can be controlled within an accuracy of 4%.rights: 社団法人日本機械学会 rights: 本文データは学協会の許諾に基づきCiNiiから複製したものである relation: IsVersionOf: http://ci.nii.ac.jp/naid/110002385062/textapplication/pdfjournal articl

Mechanisms involved in atorvastatin-induced decay-accelerating factor expression

<p><b>Copyright information:</b></p><p>Taken from "Statin-induced expression of CD59 on vascular endothelium in hypoxia: a potential mechanism for the anti-inflammatory actions of statins in rheumatoid arthritis"</p><p>Arthritis Research & Therapy 2006;8(4):R130-R130.</p><p>Published online 21 Jul 2006</p><p>PMCID:PMC1779384.</p><p></p> Human umbilical vein endothelial cells (HUVEC) were cultured for 48 hours under hypoxia (1% O) and were treated with atorvastatin (At) (0.5 μM) in the presence or absence of mevalonate (200 μM), -monomethyl-L-arginine (L-NMMA) (500 μM), -nitro-L-arginine methyl ester (L-NAME) (100 μM) and geranylgeraniol (GGOH) (20 μM). Endothelial cell CD59 expression was measured by flow cytometry using the mAb BRIC 229. Results are expressed as the percentage increase in relative fluorescence intensity above the hypoxic control (US) (= 4). *< 0.5, **< 0.01 compared with untreated controls