Application of neurite orientation dispersion and density imaging or diffusion tensor imaging to quantify the severity of cervical spondylotic myelopathy and assess postoperative neurological recovery from this myelopathy

University of Yamanashi (山梨大学)博士(医学)医工博4甲第201号thesi

[自著紹介] 『社会調査の方法論』の刊行をふり返る

departmental bulletin pape

地図DB法を用いたオムニバス調査の回答の特徴

全国調査など大型の調査票調査を独力で実施する予算がない場合，費用を抑えながら新規に調査を実施しその結果を得るには，オムニバス調査を活用するという方法がある．オムニバス調査においては，住民基本台帳等を利用して標本抽出することが現実的ではないので，住宅地図データベースをもとにして世帯の抽出がなされる（地図DB法）．しかしながら，地図DB法を用いたオムニバス調査の調査としての特徴についての研究はみられない．そこで，地図DB法を用いたオムニバス調査を実施し，その調査の実施状況を記述するとともに，回答の特徴を導出した．Omnibus surveys can be used to conduct large survey projects, such as nationwide surveys, while keeping costs down. In an omnibus survey, it is difficult to use the Basic Resident Ledger, etc., thus samplings are usually conducted based on a residential map database (the map DB method). However, no studies have been found on the characteristics of omnibus surveys that employ the map DB method. Therefore, this study explores the characteristics of an omnibus survey using the map DB method by describing and analyzing the survey responses.departmental bulletin pape

Possible association of fimA genotype of Porphyromonas gulae with the severity of periodontal disease and the number of permanent teeth in dogs

Previous research has demonstrated that Porphyromonas gulae (P. gulae) significantly contributes to the development of periodontal disease in dogs. Porphyromonas gulae is divided into three subtypes according to the 41-kDa filamentous appendage (fimA), defined as types A, B, and C. This study aimed to elucidate the association between fimA type of P. gulae with the number of permanent teeth, reflecting the severity of periodontal disease. Two hundred twenty-five dogs were categorized by P. gulae fimA type as negative, type A dominant, type B dominant, and type C dominant. The stage of periodontal disease in P. gulae-positive dogs increased with age, particularly in type C dominant dogs. Correspondingly, the number of permanent teeth in P. gulae fimA type C-dominant dogs was significantly lower than that of P. gulae-negative dogs, suggesting there is a significant association between fimA type of P. gulae and the number of permanent teeth resulting from the development of periodontal disease.journal articl

The novel sustained 3-hydroxybutyrate donor poly-D-3-hydroxybutyric acid prevents inflammatory bowel disease through upregulation of regulatory T-cells

The matrilineal transmission of maternal behavior has been reported in several species. Studies, primarily on rats, have suggested the importance of postnatal experience and the involvement of epigenetic mechanisms in mediating these transmissions. This study aims to determine whether the matrilineal transmission of maternal behavior occurs in mice and whether the microbiota is involved. We first observed that early weaned (EW) female mice showed lower levels of maternal behavior, particularly licking/grooming (LG) of their own pups, than normally weaned (NW) female mice. This difference in maternal behavioral traits was also observed in the second generation, even though all mice were weaned normally. In the subsequent cross-fostering experiment, the levels of LG were influenced by the nurturing mother but not the biological mother. Finally, we transplanted the fecal microbiota from EW or NW mice into germ-free (GF) mice raising pups. The maternal behaviors that the pups exhibited toward their own offspring after growth were analyzed, and the levels of LG in GF mice colonized with microbiota from EW mice were lower than those in GF mice colonized with microbiota from NW mice. These results clearly indicate that, among maternal behavioral traits, LG is intergenerationally transmitted in mice and suggest that the vertical transmission of microbiota is involved in this process. This study demonstrates the universality of the intergenerational transmission of maternal behavioral traits and provides new insights into the role of microbiota.journal articl

Improved Search for νμ→νe Oscillation in a Long-Baseline Accelerator Experiment

journal articl

移動通信環境下におけるOFDM通信方式のPAPR低減方式に関する研究

application/pdf三重大学大学院工学研究科博士前期課程電気電子工学専攻50thesi

山鹿野鉱山発展計画

東京帝国大学工科大学種別:卒業論文thesi

研究ノート『意識心理学および行動心理学におけるエコロジカルな立場の素描』

1991-03In view of the recent situation in which attention has been focused on 'ecological approach', the present author attempted to review its significance for psychological studies of perception and behaviour. Definition of 'ecological psychology' was referred in the first chapter. Next to it, related studies were extracted with 'ecological factors' and 'ecology' as keywords from Psychological Abstracts. Data showed that studies of the following three categories were included; 1) studies in the behavioral ecology of animals, 2) those concerned with cognitive, evaluative, attitudinal and behavioural aspects of human ecological system, and 3) those proposing theoretical framework from ecological viewpoint. These studies increased in the middle of 1970s. Ecological framework in the psychological theory of perception was discussed with special reference to J.J.Gibson's approach. 'Invariant of the ambient light array' and 'affordance' were mentioned as core concepts of his theory. Ecological framework for the study of behaviour was also discussed by comparing laboratory analysis in psychology with field observation in ecology. It was the empasized point that laboratory 'open-field' as reduced situation would force animals to disclose possible behaviour repertoires('potentiality') from innately or empirically built-in programms for adaptation while natural habitat gave 'actuality' of behavioural adaptation by its limitation. The present author thus pointed out the complementary roles of psychological and ecological studies of behaviour.departmental bulletin pape

Slow Unfolding Pathway of Hyperthermophilic Tk-RNase H2 Examined by Pulse Proteolysis Using the Stable Protease Tk-Subtilisin

The unfolding speed of some hyperthermophilic proteins is significantly slower than those of their mesostable homologues. Ribonuclease H2 from the hyperthermophilic archaeon Thermococcus kodakarensis (Tk-RNase H2) is stabilized by its remarkably slow unfolding rate. In this work, we examined the slow unfolding pathway of Tk-RNase H2 by pulse proteolysis using a superstable subtilisin-like serine protease from T. kodakarensis (Tk-subtilisin). Tk-subtilisin has enzymatic activity in highly concentrated guanidine hydrochloride (GdnHCl), in which Tk-RNase H2 unfolds slowly. The native state of Tk-RNase H2 was completely resistant to Tk-subtilisin, whereas the unfolded state (induced by 4 M GdnHCl) was degraded by Tk-subtilisin. Degradation products of Tk-RNase H2 created from pulse proteolysis during its unfolding were detected by tricine–sodium dodecyl sulfate–polyacrylamide gel electrophoresis. We identified the cleavage sites in Tk-RNase H2 by N-terminal sequencing and mass spectrometry and constructed mimics of the unfolding intermediate of Tk-RNase H2 by protein engineering. The mimics were biophysically characterized. We found that the native state of Tk-RNase H2 (N-state) changed to the IA-state that was digested by Tk-subtilisin in the early stage of unfolding. In the slow unfolding pathway, the IA-state shifted to two intermediate forms, IB-state and IC-state. The IB-state was digested by Tk-subtilisin in the C-terminal region, but the IC-state was a Tk-subtilisin resistant form. These states gradually unfolded through the ID-state, in which the N-terminal region was digested. The results indicate that pulse proteolysis, by a superstable protease, was a suitable strategy and an effective tool for analyzing intermediate structures of proteins with slow unfolding properties. We also showed that the N-terminal region contributes to the slow unfolding of Tk-RNase H2, and the C-terminal region is important for folding and stability