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創作application/pdfdepartmental bulletin pape

Chromosome-scale assembly with improved annotation provides insights into breed-wide genomic structure and diversity in domestic cats

Introduction Comprehensive genomic resources offer insights into biological features, including traits/disease-related genetic loci. The current reference genome assembly for the domestic cat (Felis catus), Felis_Catus_9.0 (felCat9), derived from sequences of the Abyssinian cat, may inadequately represent the general cat population, limiting the extent of deducible genetic variations. Objectives The goal was to develop Anicom American Shorthair 1.0 (AnAms1.0), a reference-grade chromosome-scale cat genome assembly. Methods In contrast to prior assemblies relying on Abyssinian cat sequences, AnAms1.0 was constructed from the sequences of more popular American Shorthair breed, which is related to more breeds than the Abyssinian cat. By combining advanced genomics technologies, including PacBio long-read sequencing and Hi-C- and optical mapping data-based sequence scaffolding, we compared AnAms1.0 to existing Felidae genome assemblies (20 scaffolds, scaffolds N50 > 150 Mbp). Homology-based and ab initio gene annotation through Iso-Seq and RNA-Seq was used to identify new coding genes and splice variants. Results AnAms1.0 demonstrated superior contiguity and accuracy than existing Felidae genome assemblies. Using AnAms1.0, we identified over 1.5 thousand structural variants and 29 million repetitions compared to felCat9. Additionally, we identified > 1,600 novel protein-coding genes. Notably, olfactory receptor structural variants and cardiomyopathy-related variants were identified. Conclusion AnAms1.0 facilitates the discovery of novel genes related to normal and disease phenotypes in domestic cats. The analyzed data are publicly accessible on Cats-I (https://cat.annotation.jp/), which we established as a platform for accumulating and sharing genomic resources to discover novel genetic traits and advance veterinary medicine.journal articl

Measurement of the Branching Fraction, Polarization, and CP Asymmetry for B0→ρ+ρ- Decays, and Determination of the Cabibbo-Kobayashi-Maskawa Phase ϕ2

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A Drama of Superimposed Maps: Ozu's So Far From The Land of Our Parents

2008-03-31departmental bulletin pape

GC-MS法による下水臭の分析 : ポーラスポリマーを用いるカラム吸着濃縮法の応用

application/pdfIn ordcr to establish the analytical method of odor pollutants by GC-MS, the adsorption method using a porous polymer beads was studied. Samples of emitted gas are collected by Tedler bags The odorants in sampling bags are adsorbed on Porapak Q. The trapped substances are desorbed by heating at 200℃ and injected into GC-MS. This method was applied to emitted odor from a Sewage Treatment Plant and a Nightsoil Treatment Plant. The odorants of those plants were identified as follows: toluene, chloroform, Carbontetrachloride, tetrachloroethylene, etc in Sewage Treatment Plant, and methylmercaptane, dimethylsalfide, benzene, trans-1,2-dichloroethane, etc in Nightsoil Treatment Plant.departmental bulletin pape

DataSheet_1_The Expression of Inflammasomes NLRP1 and NLRP3, Toll-Like Receptors, and Vitamin D Receptor in Synovial Fibroblasts From Patients With Different Types of Knee Arthritis.pdf

Activated rheumatoid arthritis (RA) synovial fibroblasts (SFs) are among the most important cells promoting RA pathogenesis. They are considered active contributors to the initiation, progression, and perpetuation of the disease; therefore, early detection of RASF activation could advance contemporary diagnosis and adequate treatment of undifferentiated early inflammatory arthritis (EA). In this study, we investigated the expression of nucleotide-binding, oligomerization domain (NOD)-like receptor family, pyrin domain containing (NLRP)1, NLRP3 inflammasomes, Toll-like receptor (TLR)1, TLR2, TLR4, vitamin D receptor (VDR), and secretion of matrix metalloproteinases (MMPs) in SFs isolated from patients with RA, osteoarthritis (OA), EA, and control individuals (CN) after knee surgical intervention. C-reactive protein, general blood test, anticyclic citrullinated peptide (anti-CCP), rheumatoid factor (RF), and vitamin D (vitD) in patients’ sera were performed. Cells were stimulated or not with 100 ng/ml tumor necrosis factor alpha (TNF-α) or/and 1 nM or/and 0.01 nM vitamin D3 for 72 h. The expression levels of NLRP1, NLRP3, TLR1, TLR2, TLR4, and VDR in all examined SFs were analyzed by quantitative real-time PCR (RT-qPCR). Additionally, the secretion of IL-1β by SFs and MMPs were determined by ELISA and Luminex technology. The expression of NLRP3 was correlated with the levels of CRP, RF, and anti-CCP, suggesting its implication in SF inflammatory activation. In the TNF-α-stimulated SFs, a significantly lower expression of NLRP3 and TLR4 was observed in the RA group, compared with the other tested forms of arthritis. Moreover, upregulation of NLRP3 expression by TNF-α alone or in combination with vitD3 was observed, further indicating involvement of NLRP3 in the inflammatory responses of SFs. Secretion of IL-1β was not detected in any sample, while TNF-α upregulated the levels of secreted MMP-1, MMP-7, MMP-8, MMP-12, and MMP-13 in all patient groups. Attenuating effects of vitD on the expression of NLRP3, TLR1, and TLR4 suggest potential protective effects of vitD on the inflammatory responses in SFs. However, longer studies may be needed to confirm or fully rule out the potential implication of vitD in SF activation in inflammatory arthritis. Both VDR and NLRP3 in the TNF-α-stimulated SFs negatively correlated with the age of patients, suggesting potential age-related changes in the local inflammatory responses.</p

靴音の解析(<小特集>音響利用技術-音響情報・音響エネルギー)

In this paper, shoes sounds at walking are investigated. Their sounds may show individual walking characteristics. So we can recognize someone coming and one's physical or psychological condition by the time history pattern of their sound pressures. Their sounds are different by walking style, and there are two strong sound pressure waves at one step. The first one is a wave at heel touching down the ground and the second one is a wave at the front part of shoes getting down the ground. We study on the source of the shoes sound. The vibration generates by a collision of the shoes sole and floor, and transfers to the shoes skin. The sound radiates from the shoes skin parts. And, it is investigated that the shoes sound includes air compressing sound. Using their patterns and frequency characteristics of the sound pressures, we can understand who comes up and what is one's condition.rights: 社団法人日本機械学会 rights: 本文データは学協会の許諾に基づきCiNiiから複製したものである relation: IsVersionOf: http://ci.nii.ac.jp/naid/110002386148/textapplication/pdfjournal articl