Function of clock genes in the urothelium to regulate circadian rhythm of bladder sensation : The new concept of nocturia and nocturnal polyuria

University of Yamanashi (山梨大学)博士(医学)医工博4甲第185号thesi

ホイットマン ト シュウキョウ : クサ ノ ハ オ チュウシン ト シテ

application/pdfdepartmental bulletin pape

Chromosome-scale assembly with improved annotation provides insights into breed-wide genomic structure and diversity in domestic cats

Introduction Comprehensive genomic resources offer insights into biological features, including traits/disease-related genetic loci. The current reference genome assembly for the domestic cat (Felis catus), Felis_Catus_9.0 (felCat9), derived from sequences of the Abyssinian cat, may inadequately represent the general cat population, limiting the extent of deducible genetic variations. Objectives The goal was to develop Anicom American Shorthair 1.0 (AnAms1.0), a reference-grade chromosome-scale cat genome assembly. Methods In contrast to prior assemblies relying on Abyssinian cat sequences, AnAms1.0 was constructed from the sequences of more popular American Shorthair breed, which is related to more breeds than the Abyssinian cat. By combining advanced genomics technologies, including PacBio long-read sequencing and Hi-C- and optical mapping data-based sequence scaffolding, we compared AnAms1.0 to existing Felidae genome assemblies (20 scaffolds, scaffolds N50 > 150 Mbp). Homology-based and ab initio gene annotation through Iso-Seq and RNA-Seq was used to identify new coding genes and splice variants. Results AnAms1.0 demonstrated superior contiguity and accuracy than existing Felidae genome assemblies. Using AnAms1.0, we identified over 1.5 thousand structural variants and 29 million repetitions compared to felCat9. Additionally, we identified > 1,600 novel protein-coding genes. Notably, olfactory receptor structural variants and cardiomyopathy-related variants were identified. Conclusion AnAms1.0 facilitates the discovery of novel genes related to normal and disease phenotypes in domestic cats. The analyzed data are publicly accessible on Cats-I (https://cat.annotation.jp/), which we established as a platform for accumulating and sharing genomic resources to discover novel genetic traits and advance veterinary medicine.journal articl

Measurement of the Branching Fraction, Polarization, and CP Asymmetry for B0→ρ+ρ- Decays, and Determination of the Cabibbo-Kobayashi-Maskawa Phase ϕ2

journal articl

久根鑛山報告及計畫

東京帝国大学工学部種別:卒業論文thesi

夏目漱石にみる近代的心性と自意識の劇－『門』および＜後期三部作＞における叙法と主題の相関をめぐって－

2008-03-31劇（ドラマ）departmental bulletin pape

光化学大気汚染における一過性高濃度オキシダントと被害 : 東京都世田谷区における一事例

application/pdfAt about noon on July 9, 1974, a number of pupils (109) at the Seta Junior High School, Setagaya, Tokyo, Were affected by photochemical smog. A sharp peak of oxidant concentration amounting to 0.29 ppm with a half-width duration of 15 min was recorded at 11:50 at the Tamagawa monito-ring station situated 1.7km on the windward of the school. Records of oxidant concentration-time curves at four monitoring stations on the leeward have been compared with each other including that of Tamagawa. Although the peak concentration decayed gradually with the distance from Tamagawa, principal feature was retained through ca. 10km and for ca. 50 min. The delay of time of maximum oxidant concentration at each measuring point approximately agrees with the time required for the wind to reach each measuring point which is calculated from the distance and the mean wind speed. Oxidant concentration-time curves recorded at four monitoring stations in Kawasaki, Kanagawa, the opposite side of Tama River, were also examined. In addition, the maximum (one-hour) oxidant concentrations and the directions of wind at that time measured at over thirty monitoring stations have been plotted on a map, and the transport of the air mass with high oxidant concentration has been illustrated. The rough dimension of the air mass has been estimated. The sharp peak found at Tamagawa has been ascribed to the sudden descent of air mass including oxidant of high concentration from high above the neighboring area on the windward of Tamagawa.departmental bulletin pape