Immunohistochemical detection of angiotensin II receptors in mouse cerebellum and adrenal gland using "in vivo cryotechnique"

University of Yamanashi (山梨大学)博士(医学)医工博4甲第148号　Histochemistry and Cell Biology (2013) 140:477–490　に掲載。http://dx.doi.org/10.1007/s00418-013-1084-ythesi

梁啓超の『新中国未来記』について―兆民の『三酔人経綸問答』と対照させて―

2008-03-31departmental bulletin pape

発話行為理論と「文学」の問題

application/pdf英米文学. 1984, 32, p.52-80departmental bulletin pape

Spillover and spillback risks of ectoparasites by an invasive squirrel Callosciurus erythraeus in Kanto region of Japan

Invasive organisms can alter host-parasite relationships in a given ecosystem by spreading exotic parasites and/or becoming a new reservoir for native ones. Since these problems affect management programs of the invasive host organisms, it is necessary to monitor them individually. The Pallas's squirrel Callosciurus erythraeus is an invasive arboreal mammal introduced into Japan that threatens to exacerbate ecological and public health problems by spreading native and exotic parasites. However, only limited surveys have been available especially for ectoparasites, using the traditional combing method in which the possibility of oversight is inherent. Here, we evaluated the ectoparasite occurrences in Kanto region of Japan, using the whole-shaving method as an alternative approach. As a result of examining 52 hosts from two invaded districts (Yokohama and Yokosuka), chigger mites (Leptotrombidium spp.) and fleas (Ceratophyllus anisus and Ceratophyllus indages indages) were newly recovered in addition to the previously reported tick (Haemaphysalis flava) and exotic lice (Enderleinellus kumadai and Neophaematoponis callosciuri). The parasite burdens were higher in Yokosuka and in male host individuals, affecting infracommunity richness and composition. Our findings on the variety of native and exotic ectoparasites, at higher abundances in some cases than previously known, may suggest that both the spillover and spillback risks need to be adjusted upwards.journal articl

Improved Constraints on D0-D̅0 Mixing in D0→K+π- Decays from the Belle Detector

journal articl

0812-9869-9940 (WA), Jual Keranda Mayat Girimarto

<p>0812-9869-9940 (WA), Jual Keranda Mayat Girimarto@@Jual Keranda Mayat Girimarto, Jual Keranda Mayat Pamanukan, Jual Keranda Mayat Ketenger, Jual Keranda Mayat Pamijen, Jual Keranda Mayat Pandak, Jual Keranda Mayat Purwosari, Jual Keranda Mayat Rempoah, Jual Keranda Mayat Batuanten, Jual Keranda Mayat Cipete@@keranda jenazah 1 set promo @keranda mayat dan pemandian sepaket@keranda awet kokoh anti karat@paket keranda murah@paket keranda jenazah dan pemandian 1 paket@keranda paket@paket pemandian jenazah dan keranda@pemandian jenazah@GRATIS KAIN PENUTUP KERANDA@Menyediakan berbagai kebutuhan kepengurusan jenazah@@Dengan material stainless steel, kami memproduksi KERANDA JENAZAH dan PEMANDIAN JENAZAH yang mana ANTI KARAT, KOKOH, dan JELAS KEAWETANNYA.@@Memudahkan bagi jamaah sekalian dalam kepengurusan jenazah@Dibuat dari STAINLESS STEEL sehingga tahan karat dan aman disimpan dalam ruangan@Desain yang KOKOH mampu menahan berat hingga 300kg @@Spesifikasi Singkat:@-KERANDA@Bahan : Stainless Steel 201@Dimensi : Panjang 200 cm � Lebar 65 cm -Tinggi kurungan 64cm@Beban Maximum 300 kg@@-PEMANDIAN JENAZAH@Bahan : Stainless Steel 201@Dimensi : Panjang 205cm - Lebar 75cm - Tinggi 80cm@Beban MAX : 300KG@@@#JualKerandaMayatGirimarto, #JualKerandaMayatPamanukan, #JualKerandaMayatKetenger, #JualKerandaMayatPamijen, #JualKerandaMayatPandak, #JualKerandaMayatPurwosari, #JualKerandaMayatRempoah, #JualKerandaMayatBatuanten, #JualKerandaMayatCipete</p&gt

Two-state model of bursty gene expression.

The distribution of gene expression in a population of cells is partially caused by bursts of gene expression. (A) In the two state model of bursting gene expression, a gene stochastically turns on at rate Kon and off at rate Koff. When the gene is on, it transcribes mRNA at a rate of Kt. Note that all three rates are normalised to the rate of mRNA degradation (Kd). (B) These kinetic parameters control the shape of the gene expression distribution. In this figure, we keep Kt = 100 and vary Kon and Koff. At high Kon the distribution of mRNA transcripts is similar to a Poisson distribution (i), at high Koff the distribution of mRNA transcripts is similar to a negative binomial (ii), and at low values for Kon and Koff the distribution is bimodal (iii).</p

Dual myeloid enhancer and T-cell repressor activity of element -25.

<p>A) Diagram of element -25 and deletion constructs. 5’ T-cell repressor and 3’ myeloid enhancer modules of element -25 (-25 el.) are coloured in orange and red, respectively. The presence of the conserved 5’ RUNT (5’ R), 3’ RUNT and GATA (3’ R/G) motifs is indicated. Three deletion constructs (del -25 el., 5’del -25 el. and 3’del -25 el.) were produced by restriction enzyme digestion using <i>BglII</i> (position 445) and <i>NheI</i> (positions 878 and 1388). B, C) Independent myeloid enhancer and T-cell repressor activities of 2 modules within element -25. Deletion constructs were stably transfected together with either <i>LMO2</i> proximal (pPex) or intermediate (mdp) promoter elements in myeloid progenitors 416B (B) and T-cells Molt4 (C), respectively. In all cases, mean and standard error of the means (SEM) for at least two independent stable transfections (each one performed at least in triplicate) are shown. Values are expressed relative to empty vector, pGL2 basic. **P < 0.01; ***P < 0.001; ns, not significant.</p

The 5’ T-cell repressor activity of element -25 is dependent on Runx binding.

<p>A, B, C) ChIP-Seq profile of the <i>LMO2</i> locus region for CBFB in murine thymocytes (A), RUNX3 in murine CD8+ SP T-cells (B) and RUNX1 in Jurkat cells (C). Elements previously described to be involved in the regulation of <i>LMO2</i> expression are indicated. Binding for CBF can be detected at element -25. A magnification of the region corresponding to element -25 is shown. Diagram of element -25 as previously. While binding of CBFB can be detected at both modules of element -25, RUNX1 and RUNX3 are preferentially bound to the 5‘ T-cell repressor module of element -25 (location of conserved RUNT motifs is shown in grey). D) T-cell repressor activity depends on a conserved RUNT motif. Conserved RUNT and LEF-1 motifs present in the T-cell repressor module of element -25 were mutated (5’ mRUNT -25 el. and mLEF1–25 el., respectively). Mutated constructs together with <i>LMO2</i> intermediate promoter element (mdp) were stably transfected in Molt4 T-cells and luciferase activity was measured. Mean and standard error of the means (SEM) for a representative experiment of at least two independent stable transfections (each one performed at least in triplicate) are shown. Values are expressed relative to empty vector, pGL2 basic. **P < 0.01; ***P < 0.001; ns, not significant.</p

The 3’ myeloid enhancer activity of element -25 is dependent on GATA binding.

<p>A) DNaseI-Seq profile of <i>Lmo2</i> locus region in mouse myeloid progenitors 416B cells. Elements previously described to be involved in the regulation of <i>Lmo2</i> expression are indicated. The strongest DHS in the <i>Lmo2</i> locus co-localises with element -25. A magnification of the region corresponding to element -25 is shown. Diagram of element -25 as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0131577#pone.0131577.g002" target="_blank">Fig 2A</a>. DNaseI profile shows a strong protection in the region corresponding to conserved GATA motif (location shown in grey). B, C) ChIP-Seq profile of RUNX1 and GATA2 in <i>LMO2</i> locus region in murine haematopoietic progenitors HPC7 (B) and human CD34+ (C) cells. Elements previously described to be involved in the regulation of <i>LMO2</i> expression are indicated. Strong binding for GATA2 and RUNX1 can be detected at element -25. A magnification of the region corresponding to element -25 is shown. Diagram of element -25 as previously. Binding of RUNX1 and GATA2 specifically takes place at the 3‘ myeloid enhancer module of element -25. D) Myeloid enhancer activity depends on a conserved GATA motif. Conserved RUNT and GATA motifs present in the myeloid enhancer module of element -25 were mutated (3’mRUNT -25 el. and mGATA -25 el., respectively). Mutated constructs were stably transfected together with <i>LMO2</i> proximal promoter element (pPex) in myeloid progenitor 416B cells and luciferase activity was measured. Mean and standard error of the means (SEM) for at least two independent stable transfections (each one performed at least in triplicate) are shown. Values are expressed relative to empty vector, pGL2 basic. **P < 0.01; ***P < 0.001.</p