Formation and characterization of FeLV iscoms.

Immunostimulating complexes (ISCOMs) have been prepared from feline leukaemia virus (FeLV) envelope proteins. The ISCOMs were characterized biochemically in SDS-polyacrylamide gel electrophoresis showing the presence of proteins of estimated molecular weights of 15,000, 27,000 and 70,000. Immunoblotting showed that both the transmembrane protein p15E and the external glycoprotein gp70 (making up the gp85 protein) were present in the ISCOM. Furthermore, a degradation product of gp70 with an estimated molecular weight of 32,000 was identified in the immunoblot. The FeLV ISCOM was shown by electron microscopy to have the characteristic cage-like structure of an ISCOM with a mean diameter of 37 nm. About 10% of the total amount of gp70 in the culture fluid was recovered in the ISCOMs. The largest loss was encountered during the sedimentation of the virus. In a preliminary immunization experiment in mice the FeLV ISCOMs elicited after a booster gave a clear-cut immune response against gp70

Molecular dynamics simulation of backflow generation in nematic liquid crystals

The mechanism of backflow generation in nematic liquid crystals under the application of an electric field is investigated by molecular dynamics simulation, and the roles of intermolecular interaction in the generation of bulk velocity are investigated. It is confirmed that the reorientation of molecules by the application of an electromagnetic field induces a transient “S-shaped” bulk velocity profile. The rotation and rearrangement of molecules during the reorientation process generate a local bulk velocity gradient

A one-mutation mathematical model can explain the age incidence of acute myeloid leukemia with mutated nucleophosmin (NPM1)

Acute myeloid leukemia with mutated NPM1 gene and aberrant cytoplasmic expression of nucleophosmin (NPMc+acute myeloid leukemia) shows distinctive biological and clinical features. Experimental evidence of the oncogenic potential of the nucleophosmin mutant is, however, still lacking, and it is unclear whether other genetic lesion(s), e.g. FLT3 internal tandem duplication, cooperate with NPM1 mutations in acute myeloid leukemia development. An analysis of age-specific incidence, together with mathematical modeling of acute myeloid leukemia epidemiology, can help to uncover the number of genetic events needed to cause leukemia. We collected data on age at diagnosis of acute myeloid leukemia patients from five European Centers in Germany, The Netherlands and Italy, and determined the age-specific incidence of AML with mutated NPM1 (a total of 1,444 cases) for each country. Linear regression of the curves representing age-specific rates of diagnosis per year showed similar slopes of about 4 on a double logarithmic scale. We then adapted a previously designed mathematical model of hematopoietic tumorigenesis to analyze the age incidence of acute myeloid leukemia with mutated NPM1 and found that a one-mutation model can explain the incidence curve of this leukemia entity. This model fits with the hypothesis that NPMc+acute myeloid leukemia arises from an NPM1 mutation with haploinsufficiency of the wild-type NPM1 allele

Ground State Properties of an Asymmetric Hubbard Model for Unbalanced Ultracold Fermionic Quantum Gases

In order to describe unbalanced ultracold fermionic quantum gases on optical lattices in a harmonic trap, we investigate an attractive ($U<0$) asymmetric ($t_\uparrow\neq t_\downarrow$) Hubbard model with a Zeeman-like magnetic field. In view of the model's spatial inhomogeneity, we focus in this paper on the solution at Hartree-Fock level. The Hartree-Fock Hamiltonian is diagonalized with particular emphasis on superfluid phases. For the special case of spin-independent hopping we analytically determine the number of solutions of the resulting self-consistency equations and the nature of the possible ground states at weak coupling. Numerical results for unbalanced Fermi-mixtures are presented within the local density approximation. In particular, we find a fascinating shell structure, involving normal and superfluid phases. For the general case of spin-dependent hopping we calculate the density of states and the possible superfluid phases in the ground state. In particular, we find a new magnetized superfluid phase.Comment: 9 pages, 5 figure

Vacuum extraction of premature infants

No Abstract

Tuberkulose van die nier in swangerskap

No Abstract

Development and application of a health function score system for grizzly bears (Ursus arctos) in western Alberta

The persistence of grizzly bears (Ursus arctos) in western Alberta is threatened by increasing human activities on the landscape. The Foothills Research Institute Grizzly Bear Program (FRIGBP) hypothesizes human-caused landscape change in Alberta causes long-term stress in individual bears, resulting in impaired biological functions and, when many bears are affected, decreased population performance. To facilitate the evaluation of individual grizzly bear health within the FRIGBP, the objective of my research was to develop and assess the usefulness of a health function score system for grizzly bears. From a large set of complex biological data collected from grizzly bears from 1999 to 2007, I merged 14 � constituent� variables into four health functions; growth, immunity, movement, and stress. For each health function, I calculated individual scores by adding ranked and weighted variable percentiles. I found that health function scores corresponded well with health status of individual bears based on values for multiple constituent variables. The score system facilitated quick screening of health in individual bears, identification of bears with reduced health, and comparison of health profiles between bears. I examined the usefulness of the score system by evaluating relationships presumed to exist under the working hypothesis of the FRIGBP. Results generated from health function scores were compared with those from constituent variable values using statistical and graphical techniques. I concluded that scores likely provided clearer depiction of wildlife health relationships than did constituent variables because they were not influenced by capture method, sex, or outlying observations. By using the score system, I found support for the proposed positive relationship between human-affected landscape condition and stress, but not for inverse relationships between stress and other health functions. The usefulness of the score system could be increased by minimizing use of redundant constituent variables, e.g., in growth and immunity, and removing the influence of potential confounding factors, e.g., capture

Scoliosis Review : Introduction to an evidence based approach

Scoliosis is one of the most common diagnoses found in physical therapy practice with idiopathic scoliosis being the most common form found in children. Unlike adults with scoliosis those children who are identified with scoliosis have a tendency to progress, therefore, screening plays an important role in finding early scoliosis and in planning for future scoliosis management. The evidence in support of physical therapy for scoliosis management is poor. It is very important to have a thorough knowledge of clinical courses associated with scoliosis. The aims of this paper is to review and discuss the clinical pictures of scoliosis including types, prevalence, mechanism, and screening of scoliosis as well as introduction to an evidence based physical therapy intervention.Review Articledepartmental bulletin pape

Emergence in cyber-physical systems-of-systems (CPSoSs)

The essence of the concept emergence is aptly communicated by the following quote, attributed to Aristotle, who lived more than 2000 years ago: The Whole is Greater than the Sum of its Parts