音楽と関連した場所イメージの記号化について（続） : 1992-93年調査結果の分析と2022-23年調査結果との比較

departmental bulletin pape

SKELETAL MUSCLE SYNTROPHIN INTERACTORS REVEALED BY YEAST TWO-HYBRID ASSAY

2008-08Syntrophins are the cytoplasmic peripheral proteins of dystrophin glycoprotein complex, of which five (α1, β1, β2, γ1 and γ2) isoforms have been identified so far. Respective syntrophin isoforms are encoded by different genes but have similar domain structures. At the sarcolemma of skeletal muscle, the most abundant α1-syntrophin was shown to interact at its PDZ domain with many membrane proteins. Among them, the AQP4 interaction with α1-syntrophin PDZ domain was demonstrated by a Tg mouse study, prompting us to investigate the interaction between mouse α1-syntrophin (BC018546: nt.267–492, PDZ domain) pEXP-AD502 as prey vector and mouse AQP4 (NM009700: nt.805–969) pDBLeu as bait vector by the yeast two-hybrid assay, resulting in a negative study. We further studied the binding partner of another sarcolemma located β1-syntrophin, and performed a yeast two-hybrid experiment. With human β1-syntrophin as bait and human skeletal muscle cDNA library as prey, we obtained one positive clone which turned out to be α-dystrobrevin. Although the interaction of human β1-syntrophin with α-dystrobrevin has already been shown by immunoprecipitation assay, we have here confirmed this interaction by a yeast two-hybrid experiment.departmental bulletin pape

肥料の隨伴物質として土壤に加へられた硫酸根の移動及び集積並に之が石灰,鉄,滿俺,腐植等の移動に及ぼす影響に就て(農藝化學)

application/pdfIn Japan, the amount of sulphate-ion applied to fields as an accompaniment of fertilizers, such as ammonium sulphate, calcium superphosphate and potassium sulphate has remarkably increased in recent years, and now we are apprehensive of the damage caused by the attack on rice root by free H_2S produced by the reduction of such sulphates, especially in so-called "degradated paddy soils" Thus we made a study of the absorption of sulphate-ion by soils, the state of its leaching by pot experiments and the quantity of sulphate-ion accumulated in field soils by the application of ammonium sulphate for long years, and we obtained the results such as follows. [I] Absorption of sulphate-ion: (1) When 10g. soil with 100cc of N/5 ammonium sulphate solution was shaken, and after twenty-four hours the SO_4 content of the supernatant clear liquid was analysed, the decrease of SO_4 was confirmed, though the general opinion of present days is that the sulphate and chloride ions, unlike phosphate ion, are not adsorbed by soil colloids. Thus, it was known that the original and Na-oxalate-treated soil absorbed SO_4 fairly well, but the soil washed with N/5 HCl solution to eliminate Ca-ion, absorbed SO_1 insignificantly (Fig. 1). (2) When Ca-satulated clay which had been prepared by treating bentonite with calcium chloride solution was treated with ammonium sulphate solution by the same method as described above, it absorbed a large quantity of SO_4. However, Na-clay hardly absorbed it. On the otherhand, the absorption-curves of ammonium ion in these cases were almost the same (Fig. 2). (3) The amount of sulphate ion absorbed increased almost linearly when the amount of Ca-clay added increased, while it was not so with Na-clay, H-clay and K-clay. (4) It is clear that a part of the sulphate ion is temporarily preserved in soil by combining chemically with calcium, the percentage of preservation being greater in concentrated solution than in dilute solution. From this fact, it may be supposed that this is one of the factors which cause the preserved amount of sulphate ion to be greater in paddy fields where ammonium sulphate has an opportunity of solving as comparatively concentrated solution than in ordinary farm fields. [II] Each 1 litre of penetrated water from pots of paddy state, fertilized with ammonium sulphate, calcium superphosphate and potassium sulphate was analysed weekly as for the contents of sulphate, Ca, Fe, sulphide, free H_2S etc., and the average results of two years (1951-1952) were as follows: (1) A greater part of sulphate leached within 45 or 50 days after the plantation of rice plant, and after that the remaining part went out slowly but steadily. The former seems to be a direct outgo from ammonium sulphate and potassium sulphate, whereas the latter is an outgo from Ca superphosphate applied. There was not a great difference between the leaching amounts of sulphate and chloride during the former period of growth. (2) The outgone amount of calcium was almost the same whether it might be added as sulphate or chloride, and in both cases a greater amount of calcium leached as compared with unfertilized plots. (3) The outgo of iron was greatly accelerated by the addition of sulphate, especially in planting plots during the latter period of growth. (4) The leaching amount of sulphide was extremely little as compared with the total amount of sulphates added, and the differences between the plots were not recongnized, except that the penetrating amount of sulphate plot was a little greater. [III] Soils fertilized with ammonium sulphate for 5 to 57 years and unfertilized soils in the neighborhood were collected from different parts of Japan, and compared by chemical analysis, and the following results were obtained. (1) Soils fertilized with ammonium sulphate for long years contained more sulphate when compared with unfertilized soils; the average difference being 0.017% in surface soil, 0.012% in subsoil of paddy soils and 0.02% in surface soil, 0.34% in subsoil of ordinary field soils. But the increased amount by long years fertilization with ammonium sulphate was less than ten percents of total amount of ammonium sulphate fertilized during respective period. (2) Soils fertilized with ammonium sulphate had lower Ca content and pH value, larger total acidity and lower degree of base saturation as compared with unfertilized soils and the difference is especially significant in ordinary field soils as it is naturally expected. (3) The free iron content of soils fertilized with ammonium sulphate was comparatively small, especially in paddy field soils. (4) Humus contents are generally higher in soils fertilized with ammonium sulphate as compared with unfertilized soils. This is considered to be a result of the fact that the growth of crops was better in the plots fertilized with ammonium sulphate than unfertilized plots accordingly the organic matter that remained in soils was greater in the former plots than in the latter ones. If the plots fertilized with N without sulphate were arranged, the humus contents of the plots might be higher like those of plots fertilized with ammonium sulphate, because the leaching of Ca and Fe might be violent in the latter soils.Bulletin of the Naniwa University. Ser. B, Agriculture and natural sciences. Zoology and botany. 1954, 3, p.1-22departmental bulletin pape

Physical and psychological complaints of nurses in Shinshu university hospital

総務委員会では働きやすい勤務体制の検討の一環として,当院の交替勤務を行っている婦長以外の看護婦(士),准看護婦211名を対象に勤務前後の疲労度を調査した。調査表は産業疲労の「自覚症状しらべ」の各項目に100mmのアナログスケールを付し用いた。調査の結果以下のことがわかった。1. 勤務別比較では勤務前に疲労の自覚症状の点数が一番高かったのは三交替深夜勤務で,一番低かったのは二交替夜勤であった。全体的に三交替勤務の方が二交替勤務より点数が高かった。勤務後では夜勤が一番訴えの点数が高かったが,二番目の準夜勤務との差は小さかった。2. 部署別比較では勤務前の疲労の訴えが一番高かったのはICUで,勤務後では東3階病棟であった。一番低かったのは勤務の前後とも西7階病棟であった。しかし勤務後の疲労の増加率は西7階病棟が顕著に高かった。3. 年代別比較では勤務の前後とも疲労の訴えの点数が一番高かったのは20代であった。これは日本産業衛生協会疲労研究会の調査結果とも一致している。一番低かったのは勤務前では30代,勤務後では40代であった。20代と30代,40代と50代は疲労の表れかたが類似していた。4. 項目別に自覚症状の訴えをみると,点数が高かったのは勤務の前後とも一群>二群>三群の順であった。しかし勤務後の増加率が2倍以上の項目は二群に多かった。疲労の訴え方には勤務体制,年齢以外に婚姻状況,職務満足度,職場風土等が関係していると考えられる。Article信州大学医学部附属病院看護研究集録 28(1): 181-192(1999)departmental bulletin pape

圧延軟鋼板のCuppingに伴ふ諸現象の研究

東京帝国大学工学部種別:卒業論文thesi

Additional file 3: of Rice OsRH58, a chloroplast DEAD-box RNA helicase, improves salt or drought stress tolerance in Arabidopsis by affecting chloroplast translation

Splicing efficiency of chloroplast intron-containing genes. Total RNA was extracted from 2-week-old wild type (WT) and OsRH58-expressing transgenic Arabidopsis plants (OX1,OX2, and OX3) grown on MS medium or MS medium supplemented with 150 mM NaCl or 300 mM mannitol, and the levels of unspliced and spliced transcripts of each gene were determined by real-time RT-PCR. The mean and standard error were obtained from three biological replicates. (PDF 405 kb

Additional file 1: of Rice OsRH58, a chloroplast DEAD-box RNA helicase, improves salt or drought stress tolerance in Arabidopsis by affecting chloroplast translation

Confirmation and seed germination of transgenic plants under normal, cold, or ABA conditions. (A) Expression of OsRH58 in three homozygous Arabidopsis lines (OX1, OX2, and OX3) was confirmed by RT-PCR. Actin was used as a loading control. (B) Germination rates of the wild type (WT) and transgenic plants were scored on MS medium or MS medium supplemented with 1 μM ABA at normal temperatures, and on MS medium at 10 °C. (PDF 406 kb

Additional file 5: of Rice OsRH58, a chloroplast DEAD-box RNA helicase, improves salt or drought stress tolerance in Arabidopsis by affecting chloroplast translation

List of primers used in RT-PCR and quantitate real-time RT-PCR analysis. (PDF 403 kb

Additional file 2: of Rice OsRH58, a chloroplast DEAD-box RNA helicase, improves salt or drought stress tolerance in Arabidopsis by affecting chloroplast translation

Seedling growth of the wild type and transgenic plants under normal, cold, or ABA conditions. Growth of the wild type (WT) and OsRH58-expressing transgenic Arabidopsis plants (OX1,OX2, and OX3) was analyzed on MS medium or MS medium supplemented with 2 μM ABA at normal temperatures, and on MS medium at 10 °C. The mean and standard error of fresh weight were obtained from three biological replicates. (PDF 408 kb