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横浜国立大学構内の鳥類目録

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RAPTOR Controls Developmental Growth Transitions by Altering the Hormonal and Metabolic Balance

Vegetative growth requires the systemic coordination of numerous cellular processes, which are controlled by regulatory proteins that monitor extracellular and intracellular cues and translate them into growth decisions. In eukaryotes, one of the central factors regulating growth is the serine/threonine protein kinase Target of Rapamycin (TOR), which forms complexes with regulatory proteins. To understand the function of one such regulatory protein, Regulatory-Associated Protein of TOR 1B (RAPTOR1B), in plants, we analyzed the effect of raptor1b mutations on growth and physiology in Arabidopsis (Arabidopsis thaliana) by detailed phenotyping, metabolomic, lipidomic, and proteomic analyses. Mutation of RAPTOR1B resulted in a strong reduction of TOR kinase activity, leading to massive changes in central carbon and nitrogen metabolism, accumulation of excess starch, and induction of autophagy. These shifts led to a significant reduction of plant growth that occurred nonlinearly during developmental stage transitions. This phenotype was accompanied by changes in cell morphology and tissue anatomy. In contrast to previous studies in rice (Oryza sativa), we found that the Arabidopsis raptor1b mutation did not affect chloroplast development or photosynthetic electron transport efficiency; however, it resulted in decreased CO2 assimilation rate and increased stomatal conductance. The raptor1b mutants also had reduced abscisic acid levels. Surprisingly, abscisic acid feeding experiments resulted in partial complementation of the growth phenotypes, indicating the tight interaction between TOR function and hormone synthesis and signaling in plants.journal articl

神経・筋骨格系を有する3次元股義足歩行シミュレーションモデルの開発

本研究では,股義足を使用した歩行を再現することのできるシミュレーションモデルを開発した.まず,股義足歩行の動作分析を行い,股義足歩行の特徴を調べた.その結果から,妥当な股義足歩行をシミュレーションで再現するには3次元で全身のモデルが必要であることがわかった.そこで,股義足の機械的モデルを導入した股義足・筋骨格系モデルと股離断者の神経系モデルを統合した3次元股義足歩行シミュレーションモデルを構築した.数値シミュレーションにおいて,20,000試行の神経系のパラメータ探索計算を経て,3次元,多数歩,全身動作の股義足歩行を生成した.生成された歩行は,実際の股義足歩行の特徴を定性的に再現していた.This paper describes the development of a model which simulates the walking with a hip disarticulation prosthesis (HDP). In order to characterize walking with an HDP, the motion analyses were performed and incorporated into this study. Results of these analyses suggested that a three-dimensional model of the entire body was necessary to generate an accurate walking with an HDP. Therefore, a three-dimensional neuro-musculo-skeletal model of a human wearing an HDP was proposed including the mechanical model of an HDP and a neural model of a hip disarticulation amputee. After 20,000 iteration computation for the optimization process of neural parameters, simulated walking qualitatively represents the measured characteristics and dynamics of walking with an HDP.journal articl