現代ドイツにおける｢社会的市場経済｣の変容 ―2003年閉店時開法改正論議を手がかりに―

科学研究費補助金 研究種目：基盤研究(C)(2)　課題番号：14530094　研究代表者：福澤直樹　研究期間：2002-2004年度research repor

ブロックシャフリングによる増殖可能ペプチドライブラリの作成 <平成12年度 共同研究の概要>

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Search for D0-D̅0 Mixing in D0→K+π- Decays and Measurement of the Doubly-Cabibbo-Suppressed Decay Rate

journal articl

リクジョウ ショクブツ ノ ツウスイ サイボウ ブンカ ニ カカワル NACガタ テンシャ インシ VNS ノ ヒカク ブンシ キノウ カイセキ

奈良先端科学技術大学院大学博士(バイオサイエンス)doctoral thesi

Accessory cells (ACs) of immunised IFN-γR KO mice are required for their defective Tactivity

<p><b>Copyright information:</b></p><p>Taken from "Defective CD4CD25regulatory T cell functioning in collagen-induced arthritis: an important factor in pathogenesis, counter-regulated by endogenous IFN-γ"</p><p>Arthritis Research & Therapy 2005;7(2):R402-R415.</p><p>Published online 28 Jan 2005</p><p>PMCID:PMC1065335.</p><p>Copyright © 2005 Kelchtermans et al.; licensee BioMed Central Ltd.</p> Tcells, Tcells and ACs were isolated from lymph nodes and spleen of IFN-γR KO and wild-type DBA/1 mice 21 days after immunisation with collagen type II in complete Freund's adjuvant. Mixing experiments were performed as indicated. In each set, 5 × 10CD4CD25Tcells were incubated with anti-CD3 antibody in the presence of ACs and the indicated number of CD4CD25Tcells. The percentage inhibition of the proliferation of Tcells (CD4CD25) by increasing numbers of CD4CD25Tcells is shown. The results are representative of two independent experiments

ウコギ科植物における金属集積機構の解明と放射性ストロンチウム除去技術への応用

application/pdfコシアブラのストロンチウム集積はマンガンとカルシウムにそれぞれ相関があることが判明した。ヤナギ類、コシアブラおよびタカノツメ木の樹皮中にはCaとSrを含んだ結晶状の物質が観察され、木本植物に吸収されたSrは、樹皮中で不溶性の結晶状物質を形成し、植物体内に長く留まる可能性があることが考えらえた。ヤナギ表皮部分から採取したRNAについて発現量解析を行った結果、Ca結合性タンパク質などの候補遺伝子群が得られた。菌根菌の分布を比較したところ、菌根菌が多いところはセシウム137 が多いことが示唆された。Non-radioactive Sr concentration in Chengiopanax sciadophylloides in field showed a correlation with those of Mn and Ca. With SEM－EDX observation we could find crystal compounds that contain Ca and Sr in the bark of Salix sp, Chengiopanax sciadophylloides and Eleutherococcus innovan, suggesting that Sr absorbed in trees stored in crystalized materials and stored for long period. We also identified several genes including Ca-conjugated protein as genes related for Sr accumulation in trees. Roots with higher 137Cs had higher colonization rate of AM fungi in the Chengiopanax sciadophylloides rhizosphere.2012年度～2014年度科学研究費補助金(基盤研究(B))研究成果報告書24380038research repor

Suppressive capacity of CD4CD25cells is decreased more in immunized IFN-γR KO than in wild-type mice

<p><b>Copyright information:</b></p><p>Taken from "Defective CD4CD25regulatory T cell functioning in collagen-induced arthritis: an important factor in pathogenesis, counter-regulated by endogenous IFN-γ"</p><p>Arthritis Research & Therapy 2005;7(2):R402-R415.</p><p>Published online 28 Jan 2005</p><p>PMCID:PMC1065335.</p><p>Copyright © 2005 Kelchtermans et al.; licensee BioMed Central Ltd.</p> Tcells, Tcells and accessory cells (ACs) were isolated from lymph nodes and spleen of naive IFN-γR KO and wild-type DBA/1 mice or from IFN-γR KO and wild-type DBA/1 mice 21 days after immunisation with collagen type II in complete Freund's adjuvant . In each case, a group of seven to nine mice was used. CD4CD25Tcells (5 × 10) were incubated with anti-CD3 antibody in the presence of ACs and the indicated number of CD4CD25Tcells. The percentage inhibition (100 × (Radioactivity in condition without Tcells – Radioactivity in condition with Tcells)/Radioactivity in condition without Tcells) of the proliferation of Tcells (CD4CD25) by increasing numbers of CD4CD25Tcells is shown. Two and seven independent experiments are shown in and , respectively. Each result is the mean of two cups. The means of the two (naive mice) or seven (immunised mice) independent experiments shown in and . Error bars indicate SEM

Phenotypic characterisation of CD4CD25T cells from immunised IFN-γR KO and wild-type DBA/1 mice

<p><b>Copyright information:</b></p><p>Taken from "Defective CD4CD25regulatory T cell functioning in collagen-induced arthritis: an important factor in pathogenesis, counter-regulated by endogenous IFN-γ"</p><p>Arthritis Research & Therapy 2005;7(2):R402-R415.</p><p>Published online 28 Jan 2005</p><p>PMCID:PMC1065335.</p><p>Copyright © 2005 Kelchtermans et al.; licensee BioMed Central Ltd.</p> CD4CD25T cells isolated from IFN-γR KO and wild-type mice show a similar expression pattern of activation markers, in naive and immunised conditions. CD4T cells were purified from the lymph node cells of eight IFN-γR KO and wild-type DBA/1 mice, either naive or having been immunised 21 days previously with collagen type II in complete Freund's adjuvant (purity more than 99%). CD4T cells were stained for CD25 in combination with CD69, CD62L, CD44 or cytolytic T lymphocyte-associated antigen-4 (CTLA-4). Dead cells were excluded by gating on propidium iodide-negative cells. The numbers represent the percentages of CD4CD25cells within the indicated marker. Decreased Foxp3 mRNA levels in CD4CD25Tcells from immunised mice. Lymph node cells were isolated from eight naive or immunised IFN-γR KO and wild-type DBA/1 mice. Purified CD4T cells were stained with anti-CD25-FITC and phycoerythrin-conjugated anti-CD4, and sorted. The purity of the sorted CD4CD25population was more than 97%. cDNA samples were prepared from 2 × 10cells of each population and were subjected to real-time quantitative PCR analyses. The relative quantity of Foxp3 in each sample was normalised to the quantity of β-actin. Error bars indicate standard error of the means of two (CD4CD24cells from naive mice) or three (CD4CD25cells from immunised mice) independent experiments. *< 0.05 for comparison with Foxp3 expression of cells isolated from immunised wild-type mice (Mann–Whitney -test)

子宮動脈塞栓術を施行するも静脈性出血が持続し、子宮全摘術を要した子宮破裂の1症例

technical repor

腹腔鏡下子宮筋腫核出術筋層縫合におけるSmead-Jones縫合の有用性

technical repor