Quantum transport in a normal metal/odd-frequency superconductor junction

Recent experimental results indicate the possible realization of a bulk odd-frequency superconducting state in the compounds CeCu_2Si_2 and CeRhIn_5. Motivated by this, we present a study of the quantum transport properties of a normal metal/odd-frequency superconductor junction in a search for probes to unveil the odd-frequency symmetry. From the Eliashberg equations, we perform a quasiclassical approximation to account for the transport formalism of an odd-frequency superconductor with the Keldysh formalism. Specifically, we consider the tunneling charge conductance and the tunneling thermal conductance. We qualitatively find distinct behavior in the odd-frequency case compared to the conventional even-frequency case in both the electrical and thermal current. This serves as a useful tool to identify the possible existence of a bulk oddfrequency superconducting state.journal articl

Koopman mode decomposition of oscillatory temperature field inside a room

application/pdfPhysical Review E. 2020, 102 (2), P.022210-1-022210-7journal articl

炭素・アラミド繊維棒材を補強筋に用いたコンクリート部材の耐火性能に関する研究

In order to clarify the bond behavior of FRP bars under high temperature, pull-out tests were conducted changing FRP bar type (Carbon and Aramid), temperature, and concrete strength. It is concluded that the bond behavior of FRP bars under high temperature was deteriorated by softening of resin.textapplication/pdfdepartmental bulletin pape

Simultaneous Recovery of Ammonium, Phosphorus and Potassium From Swine Wastewater

S In order to recover ammonium, phosphate and potassium from swine wastewater as magnesium phosphates, the optimal condition was investigated using synthetic swine wastewater, and under the optimal condition, recovery experiment was performed using actural swine wastewatare obtained from an outflow of a septic tank. The optimal condition to recover ammonia, phosphate and potassium simultaneously was found to be a pH of 8.0, P/(N+K) of 1.5, and Mg/P of 1.5. This optimal conditions were applied to the actuarl swine wastewater, and NH4-N, K, and P were recovered at high recovery rates of 95%, 85%, and 90%, respectively.departmental bulletin pape

Search for D0-D̅0 Mixing in D0→K+π- Decays and Measurement of the Doubly-Cabibbo-Suppressed Decay Rate

journal articl

Schematic representation of the inducible system and gene targeting strategy

<p><b>Copyright information:</b></p><p>Taken from "Reversible gene knockdown in mice using a tight, inducible shRNA expression system"</p><p></p><p>Nucleic Acids Research 2007;35(7):e54-e54.</p><p>Published online 21 Mar 2007</p><p>PMCID:PMC1874634.</p><p>© 2007 The Author(s)</p> Principle of tetR-mediated control of shRNA expression. Transcription of the RNA polymerase III-dependent promoter is blocked in cells expressing the tet repressor (tetR). Upon induction by doxycycline, tetR is removed from the tet-operator sequences (tetO) inserted into the promoter, allowing transcription of shFluc. ShRNA expression leads to RNAi-mediated knockdown of the target gene firefly luciferase. Renilla luciferase is used for reference to quantify firefly luciferase activity. Scheme of the targeting strategy. ShRNA and reporter constructs were independently inserted into the locus by homologous recombination in ES cells. Genes encoding the (Rluc) and firefly luciferases (Fluc) along with an adenovirus splice acceptor sequence and a polyadenylation signal (pA) were placed downstream of the endogenous promoter. The Fluc-specific shRNA is expressed under the control of the U6-tet or H1-tet promoter, and terminated by five thymidines (shRNA). The -sites flanking the shRNA expression cassettes were used to generate a negative control through cre-mediated recombination in ES cells. Southern blot analysis of genomic DNA from transfected ES cell clones containing the shRNA- (lane #1 and #2) or the reporter-constructs (lanes #3 and #4). Homologous recombination at the locus is detectable by using EcoRV-digested genomic DNA and probe 1, resulting in a 11.7 kb band for the wt and a 2.5 kb band for targeted allele. E: EcoRV; X: XbaI; neo: FRT-flanked neomycin resistance gene; hyg: FRT-flanked hygromycin resistance gene. () Western blot analysis from protein extracts of ES cells expressing either the wt tetR or the itetR using tetR- or β-Actin-specific antiserum. Control: protein extracts from wt ES cells

Generation of transgenic rats.

<p>The transgene construct, pTet-shIR (A), contains two expression cassettes: One expresses shRNA against the insulin receptor (shIR) under the control of the human H1 promoter carrying a tetracycline operator (tetO) sequence. The second cassette consists of a tetracycline repressor (tetR) cDNA followed by a polyadenylation site (pA) and is driven by the CAGGS promoter. An RNase protection assay (RPA) probe was designed to bind to the loop and antisense strand of the hairpin. Primers TetRfor and TetRrev (arrowheads) were used for genotyping of rats. (B) Expression of the shRNA was detected by RPA in 20 µg of total RNA isolated from white adipose tissue (WAT) of wild-type (WT) and transgenic (Tet14 and Tet29) rats treated with doxycycline (DOX, 2 mg/mL) for 4 days. M: RNA Decade marker; Y: yeast RNA; Y-: yeast RNA without RNase digestion; nt: nucleotides. (C) Expression of insulin receptor (IR), tetracycline repressor (tetR), and ß-actin were detected by Western blot in 20 µg of WAT, brain and heart protein from the same rats.</p

Effect of shRNA expression on blood glucose levels and insulin signalling.

<p>Blood glucose (A) and plasma insulin levels (B) were markedly increased in Tet14 and Tet29 transgenic rats after doxycyline treatment (DOX, 2 mg/mL for 4 days). Insulin sensitivity (C) and signalling (D) were blunted by the treatment. Blood glucose was measured before (open bars, C) and 15 min after i.p. injection of insulin (10 U/kg) (closed bars, C) after 4 days of DOX treatment. Values are given as % of baseline before insulin injection. In the same rats, total Akt and phospho Ser473-Akt (pAkt) (D) were determined by Western blot in 20 µg protein from the interscapular brown adipose tissue. * p<0.05; ** p<0.01 vs. baseline; # p<0.05; ## p<0.01 (Student's t-test). (E) The reversibility of insulin receptor knockdown was shown in three groups of Tet29 transgenic rats treated with different doses of DOX as indicated. DOX treatment was stopped when blood glucose levels reached values between 250 and 300 mg/dL and the further development of blood glucose was monitored.</p

Efficiency of H1/U6-shRNA-mediated firefly luciferase (Fluc) knockdown in mice expressing the codon-optimized tetR

<p><b>Copyright information:</b></p><p>Taken from "Reversible gene knockdown in mice using a tight, inducible shRNA expression system"</p><p></p><p>Nucleic Acids Research 2007;35(7):e54-e54.</p><p>Published online 21 Mar 2007</p><p>PMCID:PMC1874634.</p><p>© 2007 The Author(s)</p> Each configuration (control, H1- shRNA, U6 shRNA) was analyzed using two to four mice at the age of 8–10 weeks, respectively. Percentages of shRNA-mediated repression of firefly luciferase activity with standard error of the mean are shown for untreated controls (gray bars) and in animals upon 10 days feeding with 2 mg/ml doxycycline in the drinking water (white bars). In negative control animals (black bars), the shRNA expression cassettes are removed through cre-mediated recombination. Relative values of relative firefly luciferase expression level in different organs were calculated by using luciferases activities for reference. () H1 promoter-driven shRNA expression. () shRNA expression through the U6 promoter

Lack of toxicity of transgenic shRNA expression.

<p>(A) Tet29 rats were treated with doxycycline (DOX) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005124#pone-0005124-g003" target="_blank">Figure 3</a>. At the end of the experiment, 20 µg of total RNA from liver was used in an RPA for detection of mir122. M: RNA Decade marker; Y: yeast RNA; Y-: yeast RNA without RNase digestion; nt: nucleotides. Protein kinase R (PKR) expression was used as marker for interferon response in white adipose tissue of acutely (B, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005124#pone-0005124-g001" target="_blank">Figure 1</a>) or in heart of chronically (C, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0005124#pone-0005124-g003" target="_blank">Figure 3</a>) DOX treated wild-type (WT), Tet14, and Tet29 rats. PKR was detected by Western blot in 20 µg protein; an unspecific band (indicated by *) was used as loading control. HEKi: positive control, 20 µg of protein of HEK cells treated with 1 µM interferon-α2a for 24 hours.</p