ESR observation of optically generated solitons in the quasi-one-dimensional iodo-bridged diplatinum complex Pt_2(n-pentylCS_2)_4I

Light-induced electron spin resonance (LESR) measurements have been performed on a quasi-onedimensional(Q1D) iodo-bridged diplatinum complex Pt_2(n-pentylCS_2)_4I, where the thermal activation of solitons has been reported in the doubly-degenerate alternate-charge-polarization (ACP) (Pt^2+-Pt^3+-I−-Pt^3+-Pt^2+) state formed below 210 K. An enhancement of ESR signal has been detected due to the photogeneration of Pt^3+ spins below about 30 K, as confirmed through the observed g values of g∥=1.980 and g⊥=2.215 with the external field parallel and perpendicular to the chain axis, respectively. The LESR linewidth is clearly smaller than that of the ESR signal of Curie spins observed under the dark condition, whereas it exhibits uniaxial anisotropy similar to that of the dark ESR due to the anisotropic hyperfine interaction of Pt and iodine nuclear spins. The small LESR linewidth compares well with the motionally-narrowed ESR linewidth of thermally activated solitons at elevated temperatures, indicating that the photogenerated spins are mobile. Furthermore, bimolecular recombination of photogenerated spins has been demonstrated from the excitation power dependence and decay curves of LESR intensity. These LESR features strongly suggest that the mobile spins are photogenerated solitons, which agrees with theoretical predictions.journal articl

YLBSによるペプチドライブラリーの作成と利用

Peptides have a big potential to be used for biologically active molecules such as hormone, antibiotics, inhibitors and others. Therefore, they are one of the most important substances targeted by Evolutionary Molecular Engineering. The possibility of building peptide-coding DNA libraries, beginning from 20 species of amino acid-monomer blocks, by use of YLBS technology was examined. Three/ four steps of Y-ligation were performed, providing y3 N 4 DNA libraries corresponding to peptides of 8-mers/16-mers, respectively. Y3 was confirmed to be really successful in containing the whole set of 8mers. Tenacious problems of deletion and uneven appearance of blocks were challenged and fruitfully solved by introducing an operation which does not depend on the second-strand cutting of DNA by restriction enzymes or by starting with preferential blocks for T4 RNA ligation.textapplication/pdfdepartmental bulletin pape

Search for D0-D̅0 Mixing in D0→K+π- Decays and Measurement of the Doubly-Cabibbo-Suppressed Decay Rate

journal articl

Oral histology and embryology.

Includes bibliographical references and index.Book fair 2012.ix, 241 p. :This outstanding book covers all areas of oral histology and embryology pertinent to clinical dental practice. Introductory material includes a complete discussion of the structure and function of the body's cells, as well as the stages of orofacial development from conception to birth. It also covers developmental problems such as cleft lip and palate, specific phases of tooth development, and biofilm substances that form on the surface of teeth. New Clinical Comments boxes and Consider the Patient scenarios help readers apply key concepts to actual practice. Provides a timeline of head and neck structural development from conception to birth and describes possible abnormalities in development, including cleft lip and palate. Describes the definitive stages and normal/abnormal paths of tooth development and maturation. Discusses specific hard and soft oral tissues including periodontal tissues, oral mucosa, TMJ, and parts of teeth (enamel, dentin, dental pulp, cementum) to illustrate how these structures develop and are related. Each chapter begins with a helpful chapter outline and a brief overview of chapter content. Consider the Patient boxes present a short case scenario and then discuss possible solutions at the end of the chapter to demonstrate practical applications of key concepts. Self-evaluation questions at the end of every chapter help readers assess their understanding of the material. Tables and boxes throughout the text make it easy to quickly summarize important information. Clinical Comments boxes throughout the chapters present tips that help readers apply key content to everyday clinical practice. Learning Objectives at the beginning of every chapter list important topics readers should know after completing the chapter. An alphabetical list of Key Terms at the beginning of each chapter helps readers learn to use these words in the correct context within clinical practice. Features a wealth of new full-color illustrations and photographs. Evolve website includes a test bank, image collection, weblinks, and interactive student exercises

In (A), EMSA assay with probes E1-B (left panel) and E1-C (right panel) and nuclear extracts from AML14

<p><b>Copyright information:</b></p><p>Taken from "Analysis of the CCR3 promoter reveals a regulatory region in exon 1 that binds GATA-1"</p><p>BMC Immunology 2005;6():7-7.</p><p>Published online 4 Apr 2005</p><p>PMCID:PMC1080127.</p><p>Copyright © 2005 Zimmermann et al; licensee BioMed Central Ltd.</p>3D10 cells is shown. Three cold competitors were used in each case: the probe itself (E1-B-WT and E1-C-WT, respectively), the probe with the GATA site mutated (E1-B-mut and E1-C-mut, respectively) and the GATA consensus oligonucleotide (GATA cons). A representative experiment, of three similar experiments, is shown. In (B), EMSA assay with the full-length probe (E1-FL) and extracts from eosinophilic AML14.3D10 cells in the presence or absence of antibodies against GATA-1 is shown. The arrow depicts the specific band. In (C), EMSA assay with probes E1-B (left panel) and E1-C (right panel) and nuclear extracts from AML14.3D10 cells and cold competitor probe itself (CC: E1-B and CC: E1-C, respectively) or anti-GATA-1 antibody (GATA-1 atb) is shown

In (A), a schematic representation of the CCR3 exon region used for electrophoretic mobility shift assays is shown

<p><b>Copyright information:</b></p><p>Taken from "Analysis of the CCR3 promoter reveals a regulatory region in exon 1 that binds GATA-1"</p><p>BMC Immunology 2005;6():7-7.</p><p>Published online 4 Apr 2005</p><p>PMCID:PMC1080127.</p><p>Copyright © 2005 Zimmermann et al; licensee BioMed Central Ltd.</p> E1-FL is the exon 1 full-length probe. Overlapping short probes are called E1-A, E1-B and E1-C, respectively. The DNA sequence is shown with GATA sites boxed. In (B), EMSA assay with the full-length probe and extracts from eosinophilic AML14.3D10 cells is shown. As cold competitors (CC), the full-length probe and short probes E1-A through C were used. The arrow depicts the specific band. A representative experiment, of three similar experiments, is shown

In (A), a schematic representation of the transgenic construct is shown

<p><b>Copyright information:</b></p><p>Taken from "Analysis of the CCR3 promoter reveals a regulatory region in exon 1 that binds GATA-1"</p><p>BMC Immunology 2005;6():7-7.</p><p>Published online 4 Apr 2005</p><p>PMCID:PMC1080127.</p><p>Copyright © 2005 Zimmermann et al; licensee BioMed Central Ltd.</p> The genomic fragment encoding 1.6 kb of the CCR3 promoter including 60 bp of exon 1 (E1), was cloned into the Hind III and Bam HI sites upstream from the EGFP gene in the pEGFP1 vector. The positions of restriction sites are indicated. In (B), Northern blot analysis of multiple tissues from transgenic mice is shown. RNA was isolated from the spleen, lung, thymus, kidney and jejunum of CCR3/EGFP transgenic mice (lines 3.1 and 3.2). 10 μg total RNA was electrophoresed on an agarose gel, transferred to nylon membranes and probed with the SV40 polyA probe in order to detect expression of the transgene. Position of 28S and 18S is depicted with arrows. Ethidium bromide staining of the gel is also shown. In (C), immunohistochemistry using an anti-GFP antibody is shown. Organs were collected, frozen sections obtained and anti-GFP immunohistochemistry performed. Positive staining is represented as black precipitate