航空構造システムにおける不確かさと信頼性

application/pdf機械の研究. 2017, 69 (9), P.762-768journal articl

エポキシ系樹脂を用いたCMP用パッドの試作とその加工特性2

textapplication/pdfdepartmental bulletin pape

Observation of B̅0→DsJ*(2317)+K- Decay

journal articl

Solar neutrino measurements in Super-Kamiokande-II

The results of the second phase of the Super-Kamiokande solar neutrino measurement are presented and compared to the first phase. The solar neutrino flux spectrum and time variation as well as oscillation results are statistically consistent with the first phase and do not show spectral distortion. The timedependent flux measurement of the combined first and second phases coincides with the full period of solar cycle 23 and shows no correlation with solar activity. The measured 8B total flux is (2:38± 0.05(stat.)/begin+0.16 // -0.15/end (sys.)) × 10^6 cm^{-2} s^{-1} and the day-night difference is found to be (-6.3 ±4.2(stat.)±3.7(sys.))%.There is no evidence of systematic tendencies between the first and second phases.journal articl

釜石製鉄所現業従業員の賃金と職位 : 中間報告(<特集>地方産業都市の興隆と安定 : 希望学・釜石調査からの考察)

鉄鋼業合理化の一環として, 1864年から1869年にかけて, 富士製鉄株式会社釜石製鉄所(現新日本製鐵株式会社釜石製鉄所)から, 新鋭高炉を装備した東海製鉄名古屋製鉄所(現新日本製鐵株式会社名古屋製鉄所)へ, 1,400名余りの現業従業員が転属した. この転属の対象となった従業員については賃金台帳が残されており, 筆者はそれを用いて釜石製鉄所における誘因体系を再現する分析に取り組んでいる. 分析作業は継続中であり, 本稿はその中間報告である.Under the industrial policies that encouraged iron and steel companies to restructure and consolidate iron works, more than one thousand and four hundred workers moved from the Kamaishi Iron Works to Tokoai Iron Works from 1964 to 1969. Records of those workers'wages and positions covering the whole their career at Kamaishi were occasionally preserved at the Kamaishi Iron Works, and the author continues to build a database of the records for the goal to clarify the incentive mechanism then. This paper is an interim report of the research project.departmental bulletin pape

Cortical astrocytes <i>in</i><i>vivo</i> display smad1/5/8 pathway activation during early postnatal development.

<p>A. Western blotting for p-smad1/5/8 in acutely purified astrocytes across developmental time points demonstrates pathway activation during the early postnatal period. Actin bands confirm equal protein loading. B–F. Immunostaining of sagittal brain sections from Aldh1l1-eGFP mice to visualize astrocytes. Merged images show DAPI (blue), p-smad1/5 (red) and Aldh1l1-eGFP (green) (D–F). Colocalization of p-smad1/5 with eGFP+ cortical astrocytes was quantified across the first two weeks of postnatal development using ImageJ (B). N = 2 animals for each time point. Immunostaining of P6 brains sections with DAPI (blue), p-smad1/5 (red), GFAP (magenta) and Aldh1l1-eGFP (green) shows that p-smad1/5 and GFAP staining localizes predominately to astrocytes in the outer cortical layers and glia limitans (C). G–H. Sagittal brain sections from Aldh1l1-eGFP mice were sequentially immunostained for p-smad1/5 and ki67. Yellow arrows indicate cortical astrocytes that express both p-smad1/5 and ki67 (G). Colocalization of p-smad1/5 and ki67 with eGFP+ cortical astrocytes was quantified using ImageJ (H). N = 3 animals. Significance determined using students t-test. All scale bars are 25 µm. Error bars represent SEM.</p

BMP signaling activates the smad1/5/8 pathway in astrocytes and promotes a more process-bearing morphology.

<p>A. Semi-quantitative RT-PCR of acutely purified P7 rat astrocytes confirmed <i>in</i><i>vivo</i> expression of numerous TGF-β superfamily Type I and Typ1 II receptors. B–C. Western blots of purified astrocytes cultured in either HbEGF or FGF2 and then treated with 50 ng/ml of BMP5 for 2 hours show robust activation of the smad1/5/8 pathway (p-smad1/5/8) (B), but not the smad2/3 pathway (p-smad2) (C). TGF-β treated HT-1080 Cell Extract was used as a positive control for detection of smad2/3 pathway activation. Actin bands confirm equal protein loading. D–F and H–I. Images of purified astrocyte morphology using CellMask HCS staining to visualize cell membranes. Astrocytes were cultured for 3DIV in HbEGF (D), FGF2 (E), BMP5 (F), HbEGF+BMP5 (H), or FGF2+BMP5 (I). G and J. Quantification of astrocyte morphology was done using Sholl Analysis in ImageJ. Average maximum process length (G) and number of primary processes per cell (J) were analyzed. N = 3 for each condition. Significance determined using one-way ANOVA with Tukey correction. Error bars represent SEM.</p

BMP and FGF are robust trophic factors for purified astrocytes.

<p>A–D. Live/Dead viability images at 3DIV of purified astrocyte cultures with control base media (A) containing either HbEGF (B), BMP5 (C), or FGF2 (D). Green cells are alive, red cells are dead. Scale bars are 100 µm. E–I. Quantification of purified astrocyte survival at 3DIV in base media with the addition of various cytokines. 5 ng/ml HbEGF (positive control), as well as 100 ng/ml BMP5, 100 ng/ml IGF2 and 10 ng/ml FGF2 each significantly increase astrocyte viability above control (E). Within the TGF-β superfamily multiple BMP subfamily members also promoted astrocyte survival at 100 ng/ml (F). Additional FGF family members were examined, but only FGF1 also had a modest trophic effect at 10 ng/ml (G). BMP5 survival was dose dependent and trophic effects plateau at 50 ng/ml (H). No additive effects on astrocyte survival above HbEGF alone were observed with any combination of HbEGF, FGF2, and BMP5 (I). N ≥3 for each condition. Significance determined using one-way ANOVA with Dunnett correction. Error bars represent SEM.</p

BMP signaling modulates astrocyte maturation <i>in</i><i>vitro</i>.

<p>A–C. Purified astrocytes were cultured for 3DIV in HbEGF (A), BMP5 (B), or FGF2 and GFAP expression examined by immunostaining. CellMask HCS staining was used to visualize all astrocytes. Images show individual channels from the same field. Scale bars are 100 µm. The percentage of astrocytes expressing GFAP at 3DIV was quantified using ImageJ (C). D–G. Purified astrocytes were cultured for 3DIV in HbEGF or BMP5 and the expression of AQP4 and S100ß examined by immunostaining. All cultures were also stained with GFAP. Scale bars are 50 µm. H–J. The proliferative capabilities of purified astrocytes in HbEGF, BMP5 and FGF2 were quantified <i>in</i><i>vitro</i> over 7 days using clonal analysis. Average clone size was calculated for both individual growth factors (H) and combinations of factors (I). Clonal analysis was also performed on astrocytes pre-treated with BMP5 for 7DIV to examine reversibility (J). K–L. Western blots for EGFR. Protein samples collected from acutely purified astrocytes at different postnatal time points confirm strong developmental downregulation of astrocyte EGFR (K). EGFR is also strongly downregulated in astrocytes cultured with BMP5 for 6DIV, even in the presence of other growth factors (L). Actin bands confirm equal protein loading. N ≥3 for each condition. Significance determined using one-way ANOVA with Tukey correction. Error bars represent SEM.</p

Intracellular Viral Yields from Poliovirus Infections Performed in the Presence of Pharmacological Inducers and Inhibitors of Autophagy

<div><p>(A) H1 HeLa cells were treated with 10 μM tamoxifen in DMSO/EtOH or in DMSO/EtOH alone for 48 h at 37 °C. Cell numbers were determined, and triplicate plates were infected with poliovirus at an MOI of 0.1 PFU/cell for the times indicated. (B) H1 HeLa cells were treated with 50 nM rapamycin in DMSO/EtOH or DMSO/EtOH alone for 3 h before infection with poliovirus as in (A).</p> <p>(C) H1 HeLa cells were treated with 10 mM 3-methyladenine in DMSO/EtOH or DMSO/EtOH alone for 3 h before infection with poliovirus as in (A). Viral yields were determined by plaque assay in H1-HeLa cells and expressed as PFU/cell.</p></div