数学アルゴリズム問題の研究調査

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A balloon-borne very long baseline interferometry experiment in the stratosphere: Systems design and developments

application/pdfAdvances in Space Research. 2019, 63 (1), P.779-793journal articl

Observation of B̅0→DsJ*(2317)+K- Decay

journal articl

Solar neutrino measurements in Super-Kamiokande-II

The results of the second phase of the Super-Kamiokande solar neutrino measurement are presented and compared to the first phase. The solar neutrino flux spectrum and time variation as well as oscillation results are statistically consistent with the first phase and do not show spectral distortion. The timedependent flux measurement of the combined first and second phases coincides with the full period of solar cycle 23 and shows no correlation with solar activity. The measured 8B total flux is (2:38± 0.05(stat.)/begin+0.16 // -0.15/end (sys.)) × 10^6 cm^{-2} s^{-1} and the day-night difference is found to be (-6.3 ±4.2(stat.)±3.7(sys.))%.There is no evidence of systematic tendencies between the first and second phases.journal articl

癌間質由来ＫＧＦを標的とした再燃前立腺癌の新規治療戦略

application/pdf本研究により、前立腺間質PrSCではTGFβシグナルの活性化に伴う筋線維芽細胞様の分化誘導が、間質由来KGF mRNAの産生を低下させることが判明した。よって、再燃前立腺癌の増殖過程では、癌細胞を取り巻く間質細胞の種類が重要であると考えられた。Our results showed that TGFβ-induced myofibroblastic differentiation in PrSC reduced the expression of KGF mRNA. Thus, we suggest that type of stromal cells surrounding cancer cells may be important in the progression of castration resistant prostate cancer.平成21~22年度科学研究費補助金(若手研究(B))研究成果報告書21791499research repor

Serial Passage Competition Assay in HeLa Cells for Mixtures of Wild-Type and 3D-G64S Viruses Containing the 2C-F28S Temperature-Sensitive Attenuating Mutation

<div><p>One-to-one mixtures of 3D-G64S to WT virus containing the indicated 2C-F28S mutations were used to infect HeLa cells at an MOI of 0.1 PFU/cell at 32 °C. At 12 h post-infection, cell-associated RNA and virus were harvested, and the virus was titered to determine input for the next passage. This cycle was repeated three to five times, and representative gels of digestion products, with maps indicating the mutations at the 2C locus in the mixtures of viruses that were wild-type and 3D-G64S at the 3D locus, are shown: (A) wild-type, (B) 2C-F28S(1m), (C) 2C-F28S(3m). X denote the numbers of nucleotide substitutions at the 2C-F28S site, while O denotes the presence of the 3D-G64S mutation.</p><p>DOI: 10.1371/journal.ppat.0010011.g005</p></div

Pathogenesis of Mixtures of Wild-Type and 3D-G64S Viruses Containing the 2C-F28S Temperature-Sensitive Attenuating Mutation

<div><p>PVR mice were injected intramuscularly with 5 × 10⁶ (A) or 2 × 10⁸ (B) PFU of total virus. The viral inocula were 1/10 mixtures of the wild-type/3D-G64S derivatives to allow 3D-G64S viruses a chance to compete. For example, 2C-F28S(1m) was a mixture of 5 × 10⁵ PFU of 2C-F28S(1m) and 4.5 × 10⁶ PFU of 2C-F28S(1m)/3D-G64S, giving a total of 5 × 10⁶ PFU. The numbers of mice in each group, as designated by the identity of the 2C allele, were: wild-type, 26 mice; 2C-F28S(1m), 32 mice; 2C-F28S(3m), 4 and 27 mice in (A) and (B), respectively. Asterisks denote time points where statistically significant differences (<i>p</i> < 0.05, determined by two-sample Student's <i>t-</i>test, [d 2, 0.00125; d 3, 0.00125; d 4, 0.0112]) were observed between 2C-F28S(1m) and wild-type viruses. (C) Sequence analysis of RT-PCR products from viral RNA pools (“input”) used to inoculate the mice shown in (A) and from muscle tissue after development of disease (“output”). For output samples, samples from at least three mice were sequenced, and a representative example for each is shown.</p><p>DOI: 10.1371/journal.ppat.0010011.g006</p></div

Pathogenesis of Wild-Type and 3D-G64S Viruses in PVR Mice

<div><p>PVR mice were given IM injections with 5 × 10⁶ PFU of either the wild-type or 3D-G64S viruses and were monitored for symptoms of disease. Mice were scored as having symptoms when weakness was first observed in the inoculated limb. Asterisks denote time points where statistically significant differences (<i>p</i> < 0.05, determined by two-sample Student's <i>t-</i>test [d 3, 0.0025; d 4, 0.0053; d 5, 0.043]) were observed between 3D-G64S and wild-type viruses.</p><p>DOI: 10.1371/journal.ppat.0010011.g001</p></div

Viral Competition Assay in the Context of the 2C-F28S Temperature-Sensitive Attenuating Mutations

<div><p>Muscle tissue from the experiment shown in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.0010011#ppat-0010011-g006" target="_blank">Figure 6</a> was processed and the BstBI digestion assay was performed. Representative gels of digestion products, with maps indicating the mutations at the 2C locus are shown: (A) wild-type, (B) 2C-F28S(1m), (C) 2C-F28S(3m). (D) Quantitation of the gels in A–C. The percent 3D-G64S is shown, and represents an average from 5–10 mice for each condition.</p><p>DOI: 10.1371/journal.ppat.0010011.g007</p></div

Viral Competition Assay in Poliovirus Receptor-Expressing Mice

<div><p>(A) Diagram of poliovirus genome showing BstBI restriction enzyme sites within the coding region for 3D polymerase. Digestion of 1,024-bp ³²P end-labeled (*) RT-PCR products yields a 558-bp labeled band for wild-type and a 396-bp labeled band for 3D-G64S. 6-wk-old mice were given IM injections with a 1/2 mixture of wild-type to 3D-G64S poliovirus (2 × 10⁷ PFU total virus per mouse). Tissues were harvested when animals first developed paralysis, between days 3 and 5. PCR products were end-labeled with ³²P during synthesis by inclusion of a radiolabeled downstream primer and products were digested with BstBI before electrophoresis on a denaturing polyacrylamide gel. Products from muscle samples from the IM infections are shown in (B) while products from brain samples of the IM infections are shown in (C). In (D), 2-wk-old mice were given IC injections with a 1/1 mixture of wild-type to 3D-G64S virus, and brain tissue was harvested when mice became lethargic or paralyzed. For (B), (C), and (D), representative gels are shown. In (E), (F), and (G) all mouse data obtained from the types of experiments shown in (B), (C), and (D), respectively, are quantified, and show the total number of mice whose tissues contained predominately wild-type virus, 3D-G64S virus, or both. The total number of mice per condition are as follows: IM-muscle samples, 31 mice; IM-brain samples, 54 mice; IC-brain samples, 10 mice.</p><p>DOI: 10.1371/journal.ppat.0010011.g002</p></div