フッ化揮発法と溶媒抽出法のハイブリッド再処理に関するプロセスの研究

textapplication/pdfdepartmental bulletin pape

A balloon-borne very long baseline interferometry experiment in the stratosphere: Systems design and developments

application/pdfAdvances in Space Research. 2019, 63 (1), P.779-793journal articl

Observation of B̅0→DsJ*(2317)+K- Decay

journal articl

Solar neutrino measurements in Super-Kamiokande-II

The results of the second phase of the Super-Kamiokande solar neutrino measurement are presented and compared to the first phase. The solar neutrino flux spectrum and time variation as well as oscillation results are statistically consistent with the first phase and do not show spectral distortion. The timedependent flux measurement of the combined first and second phases coincides with the full period of solar cycle 23 and shows no correlation with solar activity. The measured 8B total flux is (2:38± 0.05(stat.)/begin+0.16 // -0.15/end (sys.)) × 10^6 cm^{-2} s^{-1} and the day-night difference is found to be (-6.3 ±4.2(stat.)±3.7(sys.))%.There is no evidence of systematic tendencies between the first and second phases.journal articl

都市空間管理をめぐる私益と公益の交錯の一側面 : 行訴法10条1項「自己の法律上の利益に関係のない違法」をめぐって(<特集>日本における「都市法」論の生成と展望)

都市空間は, 「多数の人々の諸活動と居住と生活の『場』」であるにもかかわらず, その法的理解にあっては, 被規制者の財産権と「公益」的規制という二面的な関係が視野の中心に置かれている. これに対して都市計画を「当該地域の空間形成に関する権限と利害についての, 地権者相互, あるいは地権者と非地権者住民との間における, 調整・分配ルール」とみなす立場からは, 被規制地権者以外の当該空間に対するステークホルダーの利害をどのように法的に構成し, 実体法的・訴訟法的にどのような位置づけを与えるかが, 理論的課題となってくる. 本稿は, 上のような問題意識を念頭に置きつつ, 行政事件訴訟法10条1項の主張制限「自己の法律上の利益に関係のない違法」に関する解釈論を分析したものである. 同条については, 行政処分の名宛人と第三者とを区別し, 前者は公益に関わる要件も主張できるが, 後者は個別的利益を保護し原告適格の基礎となる根拠規定違反に限り主張できるとする立場が裁判例上有力である. 他方, 近時の学説は, 名宛人-第三者のかかる二元論を相対化し, 第三者に対しても, 「公益上の理由による受忍」という構成をとることが可能な場合があることを指摘する. 都市空間に関わるステークホルダーの立場を法的に構成していく上で, この発想は有益である.City planning regulations should function as a set of rules for coordinating and distributing various interests surrounding a particular urban space. The actual implementation of regulations, however, tends to focus on the bilateral relationship between property rights and the "public interest", putting the interests of other stakeholders out of sight. The Administrative Case Litigation Act, Art.10 para.1, does not allow a plaintiff to allege illegality of an administrative disposition when such illegality has no relationship to the plaintiff's own interest. Recent attempts to re-interpret this article shed some light on the possibility of constructing such a stakeholder's interests as legally protected interests, upon which the administrative disposition infringes.departmental bulletin pape

Multilabeled Pyrene-Functionalized 2‘-Amino-LNA Probes for Nucleic Acid Detection in Homogeneous Fluorescence Assays

Homogeneous fluorescence assays for detection of nucleic acids are widely used in biological sciences. Typically, probes such as molecular beacons that rely on distance-dependent fluorescence quenching are used for such assays. Less attention has been devoted to tethering a single kind of fluorophores to oligonucleotides and exploiting hybridization-induced modulation of fluorescence intensity for nucleic acid detection. Herein, thermal denaturation experiments and fluorescence properties of oligodeoxyribonucleotides containing one or more 2‘-N-(pyren-1-yl)carbonyl-2‘-amino-LNA monomer(s) X are described. These pyrene-functionalized 2‘-amino-LNAs display large increases in thermal stability against DNA/RNA complements with excellent Watson−Crick mismatch discrimination. Upon duplex formation of appropriately designed 2‘-N-(pyren-1-yl)carbonyl-2‘-amino-LNA probes and complementary DNA/RNA, intensive fluorescence emission with quantum yields between 0.28 and 0.99 are observed. Quantum yields of such magnitudes are unprecedented among pyrene-labeled oligonucleotides. Molecular modeling studies suggest that the dioxabicyclo[2.2.1]heptane skeleton and amide linkage of monomer X fix the orientation of the pyrene moiety in the minor groove of a nucleic acid duplex. Interactions between pyrene and nucleobases, which typically lead to quenching of fluorescence, are thereby reduced. Duplexes between multiple modified probes and DNA/RNA complements exhibit additive increases in fluorescence intensity, while the fluorescence of single stranded probes becomes increasingly quenched. Up to 69-fold increase in fluorescence intensity (measured at λem = 383 nm) is observed upon hybridization to DNA/RNA. The emission from duplexes of multiple modified probes and DNA/RNA at concentrations down to less than 500 nM can easily be seen by the naked eye using standard illumination intensities

LNAzymes: Incorporation of LNA-Type Monomers into DNAzymes Markedly Increases RNA Cleavage

Incorporation of two α-L-LNA/LNA nucleotides into each of the two binding arms of a “10-23” DNAzyme has been accomplished and the RNA cleavage with these novel LNAzymes studied. In comparison with the unmodified DNAzyme, the LNAzymes show significantly improved cleavage of the phosphodiester backbone at the target nucleotide in a small RNA substrate (58n RNA) under single-turnover conditions. The LNAzymes show efficient multiple turnover. With the LNAzymes, efficient cleavage was accomplished also of a naturally occurring ribosomal RNA at a target site within a highly structured region. The reference DNAzyme was ineffective at cleaving the ribosomal RNA target