Optimization of a Seeded Free-Electron Laser with Helical Undulators

Seeded single pass free-electron lasers are promising coherent, short-duration, and intense light sources, from the visible to x rays. Operated with adjustable undulators, they are also a unique device for providing fully variable polarized radiation. We report here the first seeding of helical undulators with a variable polarized source. We demonstrate that the adjustment of the seed polarization and focusing allows the free-electron laser radiation to be optimized in terms of intensity and quality.journal articl

Hybrid Maximum Clique Algorithm Using Parallel Integer Programming for Uniform Test Assembly

Educational assessments often require uniform test forms, for which each test form has equivalent measurement accuracy but with a different set of items. For uniform test assembly, an important issue is the increase of the number of assembled uniform tests. Although many automatic uniform test assembly methods exist, the maximum clique algorithm (MCA)-based method is known to assemble the greatest number of uniform tests with the highest measurement accuracy based on the item response theory. In that method, the graph is constructed by sequentially adding a randomly formed test as a vertex without considering the graph structure. However, an important difficulty is its high space complexity, which interrupts search cliques with more than a hundred thousand vertices. To overcome this difficulty, this article proposes a new uniform test assembly algorithm: hybrid maximum clique algorithm using parallel integer programming. The first step searches a maximum clique that is as large as possible up to computer memory limitations using a state-of-the-art MCA with low time complexity but with high space complexity. The second step repeatedly searches a vertex connected with all vertices of the current maximum clique from the remaining vertices using integer programming with low space complexity but with high time complexity. The proposed method constructs a larger number of tests than the traditional methods do. Finally, we use simulation and actual data experiments to demonstrate the effectiveness of the proposed method. Results show that our method assembles a 1.5–2.7 times greater number of uniform tests than traditional methods can.journal articl

実橋計測に基づくモジュラー型ジョイントの騒音特性の解明

The field measurement of noise and vibration in a PC box-girder bridge with the modular type expansion joint was conducted in order to investigate the characteristics and mechanism of the noise generated from the modular joint possible sound radiating vibration modes of this structure under a vehicle passage. Three components of noise from modular joint are discussed by paying attention to non-stationary phenomenon of the noise generation and propagation. Effects of bridge type, car type and car running speed are also studied on the noise characteristics.textapplication/pdfdepartmental bulletin pape

Observation of B+→ΛΛ̅K+

journal articl

ナイルデルタ流域における安価な水質浄化技術の導入とその評価に関する研究

宮崎大学博士(工学)2016年度doctoral thesi

Photoactivation of the Ni-SIr state to the Ni-SIa state in [NiFe] hydrogenase: FT-IR study on the light reactivity of the ready Ni-SIr state and as-isolated enzyme revisite

The Ni-SIr state of [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki F was photoactivated to its Ni-SIa state by Ar(+) laser irradiation at 514.5 nm, whereas the Ni-SL state was light induced from a newly identified state, which was less active than any other identified state and existed in the as-isolated enzyme.journal articl

Structural requirements for B cell to myeloid reprogramming potential of C/EBPβ.

<p>Schematic representation of the different C/EBPβ constructs (left) indicating the conserved regions (CRs) in the transactivation domain (TAD; CR1,2,3,4; green, turquoise), regulatory domain (RD; CR5,6,7; red), bZip domain (yellow), and the low complexity regions (LCRs, grey). Expression of lineage specific markers: B cell CD19 (red), myeloid CD11b (blue), or double positive (magenta) at 6 (middle panel) or 9 dpi (right panel). Bar graph shows percentage of GFP⁺ gated (virus infected) cell population; B cells - control uninfected GFP^– B cell progenitors. Results represent mean ± SEM from at least two experiments.</p

C/EBPβ PTM site mutations affect lympho-myeloid trans-differentiation.

<p>A. Schematic representation of C/EBPβ PTM sites and mutants tested in B-D. B. Expression of Ly-6C, M-CSFR and Ly-6G on the reprogrammed cells at 9 dpi. C. Distribution of the different myeloid populations among the reprogrammed GFP⁺ CD11b⁺ cells, stained as in B and presented as mean ± SEM. D. Cytospins of trans-differentiated sorted cells. Experiments were repeated two to three times and similar results were obtained. Gating strategies and abbreviations as in Fig. 3. E. Schematic representation of the normal hematopoiesis and lympho-myeloid reprogramming by C/EBPβ. MPP - multi potent progenitors, CLP - common lymphoid progenitor, CMP - common myeloid progenitor, GMP - granulocyte/macrophage progenitor, iMΦ and rMΦ - inflammatory and resident monocytes/macrophages.</p

C/EBPβ WT and mutants differentially regulate key myeloid genes.

<p>RNA counts for pro-inflammatory M1, anti-inflammatory M2 and other key monocyte/macrophage genes evaluated on CD11b⁺ reprogrammed <i>C/EBPβ</i>^−/− B cell progenitors. Data were calculated as log₂ and subjected to hierarchical clustering. Results represent expression profiles from three independent experiments. On the right, comparison to data obtained from reprogramming of pre-B cell line by C/EBPα is presented (Bussmann et al., 2009). MPh - WT bone marrow-derived macrophages.</p

C/EBPβ structural mutants define distinct myeloid cell trans-differentiation outcomes.

<p>A. Representative FACS plots depicting the expression of myeloid cell markers Ly-6C, M-CSFR, and Ly-6G on 9 days trans-differentiated cells. FACS plots represent GFP⁺ CD11b⁺ cell populations, for MSCV control - GFP⁺ CD19⁺ cells. B. Distribution of the myeloid subpopulations among the reprogrammed GFP⁺ CD11b⁺ cells after staining as in A and presented as mean ± SEM. N - number of repetitions. Gr - neutrophil granulocytes, iM and rM - inflammatory and resident monocytes/macrophages, respectively, DC - dendritic cells. C. Expression of the DC markers CD11c, MHC-II and CD86 on the reprogrammed Ly-6C^– M-CSFR^– cells. Histograms represent GFP⁺ CD11b⁺ Ly-6C^– M-CSFR^– gated cells (color coded as the corresponding population on the Ly-6C/M-CSFR plot in A). “++”, “+”, “med” and “−” represent the expression levels of MHC-II and CD86 antigens. D. Cytospins of control MSCV infected CD19⁺ cells and CD11b⁺ cells reprogrammed by WT C/EBPβ or deletion mutants. B – B cells, M – macrophages, Gr – neutrophil granulocytes, * - monocytes/DCs.</p