Crystal Structure and Stoichiometric Composition of Potassium-Intercalated Tetracene

application/pdfInorganic Chemistry. 2020, 59 (17), P.12545-12551journal articl

Search for \bar{B}^0 →Λ_c^+\bar{Λ}_c^- decay at Belle

We search for the doubly charmed baryonic decay \bar{B}^0 →Λ_c^+\bar{Λ}_c^-, in a data sample of 520×106 B\bar{B} events accumulated at the Υ(4S) resonance with the Belle detector at the KEKB asymmetric-energy e^+e^- collider. We find no significant signal and set an upper limit of B(\bar{B}^0 →Λ_c^+\bar{Λ}_c^-)< 6.2×10^{-5} at 90% confidence level. The result is significantly below a naive extrapolation from B(B^-→　Ξ\begin0\c\end\bar{Λ}_c^-) assuming a simple Cabibbo-suppression factor of |V_cd/V_cs|^2. The small branching fraction may be attributed to a suppression due to the large momentum of the baryonic decay products, which has been observed in other charmed baryonic two-body B decays.journal articl

Evidence for Direct CP Violation in B0→K+π-Decays

journal articl

The expression of <i>mil-1</i>, <i>Blimp1</i>, and <i>Stella</i> become upregulated during PGC development.

<p>(A, B, C) Quantitative RT-PCR analysis of the expression of (A) <i>mil-1</i>, (B) <i>Blimp1</i>, and (C) <i>Stella</i> was performed using epiblasts (E6.0) and PGCs (E7.5 and E9.0). Histograms represent relative expression levels of these three genes at each developmental stage. The averages of expression levels in the epiblasts (E6.0) were set as 1.0. <i>Gapdh</i> PCR signal was used as an internal control to measure relative. The data were obtained from three individual embryos. *p<0.05. Error bars represent SEM.</p

Flanking regions of the PGC-specific genes also become hypomethylated during PGC development.

<p>(A) Comparison of the <i>Ifitm</i> genes consensus element (ICE) of <i>mil-1</i>/<i>Ifitm3 </i><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046036#pone.0046036-Tanaka3" target="_blank">[25]</a> with the homologous sequences found in the putative regulatory regions flanking <i>Blimp1</i>/<i>Prdm1</i>, <i>Prdm14</i>, <i>Stella</i>/<i>Dppa3</i>, and <i>Nanos3</i>. (B) Bisulfite sequencing analysis of the flanking regions of <i>Blimp1</i>, <i>Stella</i>, and <i>Dazl</i> was performed using epiblasts (E6.0 and E6.75) and PGCs (E7.5 and E10.5). The flanking regions of <i>Blimp1</i> and <i>Stella</i>, like those in <i>mil-1</i>, were also progressively demethylated during PGC development, whereas that of <i>Dazl</i> was maintained hypermethylated in PGCs at E10.5.</p

Knockdown of <i>Dnmt1</i> causes hypomethylation of <i>Blimp1</i> and <i>Stella</i> flanking regions and upregulation of <i>Blimp1</i> and <i>Stella</i> expression in ES cells.

<p>(A) Bisulfite sequencing analysis of the flanking regions of <i>Blimp1</i> and <i>Stella</i> and (B) quantitative RT-PCR analysis of <i>Blimp1</i> and <i>Stella</i> expression were performed using ES cells with or without <i>Dnmt1</i> knockdown treatment (<i>Dnmt1</i> KD/Con KD).</p

Repression of somatic gene expression does not depend on DNA methylation in PGCs.

<p>(A) Bisulfite sequencing analysis of the flanking regions of <i>Hoxa1</i>, <i>Hoxb1</i>, and <i>Gfap</i> was performed using epiblasts (E6.0), epiblast stem cells (EpiSCs), and PGCs (E13.5), showing hypomethylation in PGCs. (B) Bisulfite sequencing analysis of the regulatory region of <i>Gfap</i> and (C) quantitative RT-PCR analysis of the <i>Gfap</i> expression were performed using ES cells with or without <i>Dnmt1</i> knockdown treatment (<i>Dnmt1</i> KD/Con KD).</p

DNA demethylation of the regulatory region of <i>mil-1</i> resulted in upregulation of its expression in ES cells.

<p>(A) Bisulfite sequencing analysis of the regulatory region of <i>mil-1</i> and (B) quantitative RT-PCR analysis of <i>mil-1</i> expression were performed on embryonic stem (ES) cells with or without siRNA-mediates <i>Dnmt1</i> knockdown (<i>Dnmt1</i> KD/Con KD). (A) The regulatory region became more hypomethylated following <i>Dnmt1</i> knockdown. (B) Histogram represents the relative expression level of <i>mil-1</i> in the <i>Dnmt1</i>-knockdown ES cells. The expression level in the control ES cells (Con KD) was set as 1.0. <i>Gapdh</i> PCR signal was used an internal control to measure relative expression. The data were obtained from four independent experiments. *p<0.05. Error bars represent SEM. (C) Luciferase activity of the reporter vectors with methylated or unmethylated regulatory region of <i>mil-1</i> in ES cells. Luciferase activity was normalized against the activity of a cotransfected <i>Renilla</i> construct. The liciferase activity of the methylated construct (Methylated 3.0k-pCpGL) was set as 1.0. The data were obtained from six independent experiments. *p<0.05. Error bars represent SEM.</p