これまでの研究をふりかえって : 稚拙ながら楽しく進めてきた40年の述懐(藤澤弘介教授退職記念特集)<数学・自然科学>

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Observation of D_s1(2536)^+ → D^+π^-K^+ and angular decomposition of D_s1(2536)^+ →D^*+K_S^0

Using 462 fb^{-1} of e^+e^- annihilation data recorded by the Belle detector, we report the first observation of the decay D_s1(2536)^+　→　D+π^-K^+. The ratio of branching fractions \frac{B(D_s1(2536)^+ → D^+π^- K^+}{B(D_s1(2536)^+ → D+K^0}is measured to be (3.27±0.18±0.37)%. We also study the angular distributions in the D_s1(2536)^+　→D*+K_S^0 decay and measure the ratio of D- and S-wave amplitudes. The S-wave dominates, with a partial width of Γ_S/Γtotal=0.72±0.05±0.01.journal articl

Evidence for B0→ρ0π0

journal articl

【外国語要旨】 Analyzing teachers' beliefs using PAC analysis and questionnaires reciprocally: beliefs of a Thai Japanese-language teacher

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Absence of -methylation of promoter sequences in F1 hybrids carrying PTGS and TGS epialleles

<p><b>Copyright information:</b></p><p>Taken from "The -silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco"</p><p>Nucleic Acids Research 2006;34(8):2280-2293.</p><p>Published online 2 May 2006</p><p>PMCID:PMC1456325.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> Cytosine methylation was studied in the 35S promoter by DNA gel-blot hybridization of restricted DNAs. The sizes of the restriction fragments were calculated from the T-DNA sequence (Figure 1). The 0.8 kb fragment corresponds to the non-methylated BglII/TaiI genomic fragment. The positions of the locus-specific bands after separation of the loci with methylation-insensitive BglII enzyme are marked by arrows. Symbols above the blots indicate digestion with the methylation-insensitive enzyme only (−) or in combination with the methylation-sensitive TaiI enzyme (+)

Distribution and density of cytosine methylation in the posttranscriptionally (HeLo1) and transcriptionally (HeLo1E) silenced epialleles of tobacco

<p><b>Copyright information:</b></p><p>Taken from "The -silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco"</p><p>Nucleic Acids Research 2006;34(8):2280-2293.</p><p>Published online 2 May 2006</p><p>PMCID:PMC1456325.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> () Genomic sequencing showing the methylation distribution along the 35S promoter and the 5′-II-coding region. The sequences obtained were processed through the Methtools software (). The colored dots and horizontal grey bar indicate the positions of the methylated cytosines in trinucleotide contexts and the position of the core 35S promoter, respectively. TSS, transcription start site. () Percentages of methylated cytosines in symmetrical (CG, CNG) and non-symmetrical (CNN) contexts for the promoter (−400/+1) and the 5′-II-transcribed region (+1/+400). Data were assembled from 21 HeLo1 and 9 HeLo1E clones, each representing a pattern in individual cell

Methylation analysis of transgene loci in parental lines, F1 hybrids and subsequent generations

<p><b>Copyright information:</b></p><p>Taken from "The -silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco"</p><p>Nucleic Acids Research 2006;34(8):2280-2293.</p><p>Published online 2 May 2006</p><p>PMCID:PMC1456325.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> DNA gel-blot hybridization was carried out using the II gene probe. Genomic DNAs were predigested with methylation-insensitive enzymes (−) to obtain locus-specific bands (indicated by arrows). Absence of digestion of locus-specific bands with methylation-sensitive enzymes (+) is indicative of transgene methylation. Expected lengths of restriction fragments are given in . (–) Progeny of plants that inherited epiallellic variants of locus 1 and locus 2 (Lo1/Lo2 and Lo1E/Lo2). () Progeny of plants that inherited epiallelic variants of locus 1 and locus B (Lo1/LoB and Lo1E/LoB). (A) CG methylation in the II-coding sequence. DNAs were digested with methylation-insensitive EcoRV followed by methylation-sensitive SmaI. (B) CG methylation in the non-transcribed downstream region. DNAs were digested with methylation-insensitive EcoRV followed by methylation-sensitive Eco47III. (C) Non-symmetrical methylation in the II-coding sequence. DNAs were digested with methylation-insensitive EcoRV followed by methylation-sensitive BamHI. The sequence context C within the BamHI site is CCC and CCT on the top and bottom strand, respectively. (D) CG methylation in the II-coding sequence. To separate the loci in Lo1E/LoB and Lo1/LoB hybrids, genomic DNA was digested with the HindIII/BglII enzymes and afterwards with the methylation-sensitive SmaI

Expression analysis of the II reporter gene in the parental and hybrid lines

<p><b>Copyright information:</b></p><p>Taken from "The -silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco"</p><p>Nucleic Acids Research 2006;34(8):2280-2293.</p><p>Published online 2 May 2006</p><p>PMCID:PMC1456325.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> () Representative RNA gel-blot hybridizations of the II transcripts in silenced and non-silenced lines. Five micrograms of total RNA was loaded per lane and hybridized to the II DNA probe. RNA samples from paternal HeLo2 and HeB plants have been loaded to better compare hybridization bands intensities. The 25S rRNA ethidium bromide-stained bands are shown as loading controls. () NPTII protein accumulation level determined by ELISA. The silenced lines generally had NPTII protein levels below the detection limit of the method (5 ng/mg protein). The data represent averages from two to three ELISAs with two different protein extracts isolated from the same plant

Schematic representation of the genomic organization of transgenic loci and physical maps of the restriction sites

<p><b>Copyright information:</b></p><p>Taken from "The -silencing capacity of invertedly repeated transgenes depends on their epigenetic state in tobacco"</p><p>Nucleic Acids Research 2006;34(8):2280-2293.</p><p>Published online 2 May 2006</p><p>PMCID:PMC1456325.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> The transgenic locus 1 has been previously reported as locus X (). The methylation analysis of the promoter involved the three TaiI sites; the diagnostic sites for the analysis of the II-transcribed region were SmaI and BamHI, and Eco47III for the non-transcribed sequences at the right border. Evidence for the IR character of the T-DNA insertions in locus 1 and locus 1E has been given elsewhere (). EcoRV, BglII, and HindIII enzymes were used to dissect particular subregions of T-DNA. , promoter of the cauliflower mosaic virus; II, neomycin phosphotransferase II gene; RB, T-DNA right border; 3′chs, transcription termination sequence from the 3′-untranslated region of the chalcone synthase gene from snapdragon ()