10 research outputs found
A Critical Analysis of Multicultural Education Policies in Korea : Focusing on Official Discourses About Multicultural Students
application/pdf言語文化学研究 言語情報編. 2021, 16, P.1-16departmental bulletin pape
Observation of D_s1(2536)^+ → D^+π^-K^+ and angular decomposition of D_s1(2536)^+ →D^*+K_S^0
Using 462 fb^{-1} of e^+e^- annihilation data recorded by the Belle detector, we report the first observation of the decay D_s1(2536)^+ → D+π^-K^+. The ratio of branching fractions \frac{B(D_s1(2536)^+ → D^+π^- K^+}{B(D_s1(2536)^+ → D+K^0}is measured to be (3.27±0.18±0.37)%. We also study the angular distributions in the D_s1(2536)^+ →D*+K_S^0 decay and measure the ratio of D- and S-wave amplitudes. The S-wave dominates, with a partial width of Γ_S/Γtotal=0.72±0.05±0.01.journal articl
A Versatile Surface Design to Disperse Nanoparticles in Ionic Liquids and Organic Solvents
Thiocholine bromide was proposed as a cationic capping ligand for a variety of metal and semiconductor nanoparticles with a good dispersibility in an ionic liquid (IL). The stability of NPs was improved in the IL compared to that in water, which was verified by an improved emission property of semiconductor nanoparticles and higher thermal stability of gold nanoparticles. The cationic thiocholine was further demonstrated as a surface ligand for CdTe nanoparticle dispersible in organic media in combination with various anions.Thiocholine bromide was proposed as a capping ligand for a variety of metal and semiconductor nanoparticles with a good dispersibility in an ionic liquid. The cationic thiocholine further served as a surface ligand for CdTe NPs dispersible in organic media in combination with various anions.journal articl
Comparison of the replication capacity of the HIV-lhNef escape viruses and continued evolution
<p><b>Copyright information:</b></p><p>Taken from "Hairpin-induced tRNA-mediated (HITME) recombination in HIV-1"</p><p>Nucleic Acids Research 2006;34(8):2206-2218.</p><p>Published online 2 May 2006</p><p>PMCID:PMC1456326.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> () SupT1 cells were infected with an equimolar mixture of the five AS escape variants (5 ng CA-p24 of AS44, AS19, AS15, AS11 and AS8) that were produced in HEK293T cells. Virus was passaged at the peak of infection. Cellular DNA was extracted at days 5, 12, 16, 22, 27 and 33 post-infection and proviral DNA around the hairpin insert was PCR amplified with primers tTA1 and CN1 (see ). On the right are indicated the Smart DNA Ladder sizes (bp). () The PCR products from samples obtained at day 5, 22 and 33 were cloned in the pCR2.1 vector, and 18 or 19 clones were sequenced per sample. The composition of the virus mixture is indicated by the number of clones. The input virus names are in boldface. Newly evolved sequence variants are shown in (C). The free energy Δ value is a thermodynamic stability score of the perfect duplex structure, e.g. 44 bp for AS44 and 11 bp for AS11. () Sequence alignment of HIV-lhNef escape viruses and deletion variants asNef sequences are bold caps, tRNA sequences are lower-case italics. Homologous sequences between the tRNA and HIV-lhNef at the recombination junctions are boxed in blue. Repeated sequences at the recombination junctions are underlined
HITME recombination mechanism by a template switch during plus-strand synthesis
<p><b>Copyright information:</b></p><p>Taken from "Hairpin-induced tRNA-mediated (HITME) recombination in HIV-1"</p><p>Nucleic Acids Research 2006;34(8):2206-2218.</p><p>Published online 2 May 2006</p><p>PMCID:PMC1456326.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> Reverse transcription is initiated as described in . In this model, we assume that RT is able to copy the complete lhNef during minus-strand cDNA synthesis, but the enzyme is paused by the long hairpin during plus-strand DNA synthesis. A tRNA is subsequently used by RT as template. The complementarity between the tRNA and the viral DNA facilitates this process. Upon completion of reverse transcription, the proviral DNA is a hybrid between a plus-strand DNA with an incorporated tRNA sequence, and a minus-strand cDNA containing lhNef. The hybrid provirus structure will be resolved by DNA repair and/or subsequent cell division
Mechanistic model for HITME recombination by a second tRNA priming event during minus-strand DNA synthesis
<p><b>Copyright information:</b></p><p>Taken from "Hairpin-induced tRNA-mediated (HITME) recombination in HIV-1"</p><p>Nucleic Acids Research 2006;34(8):2206-2218.</p><p>Published online 2 May 2006</p><p>PMCID:PMC1456326.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> Reverse transcription is initiated by the tRNA primer that is annealed to the PBS. Strong-stop minus-strand DNA (ss-DNA) is synthesized by RT by copying the 5′ R region. The ss-DNA is translocated to the homologous 3′ R sequence (first strand transfer), and reverse transcription continues. The extended lhNef hairpin will cause pausing of the RT enzyme. A spurious tRNA acts as a second primer for minus-strand DNA synthesis. The ppt primes the synthesis of strong-stop plus-strand DNA (ss+DNA), which is translocated to the 5′ end of the minus-strand cDNA (second strand transfer). The minus-strand cDNA and the 2nd tRNA primer are copied during continued plus-strand DNA synthesis. Sequence complementarity between plus-strand copy of the 2nd tRNA primer and the minus-strand DNA facilitates a third strand transfer that is needed to complete reverse transcription. The non-natural priming and strand transfer events result in insertion of the tRNA sequence into the viral genome and deletion of the lhNef sequence. The tRNA sequence is in red and complementary nucleotides between tRNA and HIV-lhNef are boxed in blue
Alignment of recombination junctions between HIV-lhNef and AS escape variants
<p><b>Copyright information:</b></p><p>Taken from "Hairpin-induced tRNA-mediated (HITME) recombination in HIV-1"</p><p>Nucleic Acids Research 2006;34(8):2206-2218.</p><p>Published online 2 May 2006</p><p>PMCID:PMC1456326.</p><p>© The Author 2006. Published by Oxford University Press. All rights reserved</p> In all alignments, the upper sequence represents HIV-lhNef and the lower sequence the AS escape variant. Escape variant AS44 evolved further into AS15 and both are presented in the same alignment. The middle sequence in the AS11, AS8 and AS19 alignments is the antisense sequence of the tRNA that is inserted in the viral genome. Homologous sequences between the tRNA and HIV-lhNef are boxed in blue, the UGG sequences at recombination junctions are shaded in grey, repeated sequences at the recombination junctions of HIV-lhNef and the AS mutants are underlined. The vertical lines between the sequences indicate identity. Slashes represent sequences that are not shown. Deleted sequences are presented as dashes, asNef sequences are bold caps, Nef sequences are normal caps, tRNA sequences are bold lower-case italics. Nucleotide numbers above the upper sequence refer to the position on the genomic HIV-1 RNA transcript, with +1 being the capped G residue. Numbers in the middle sequence of the lower three panels refer to tRNA positions
Additional file 1: of The HYSTER study: the effect of intracervically administered terlipressin versus placebo on the number of gaseous emboli and fluid intravasation during hysteroscopic surgery: study protocol for a randomized controlled clinical trial
SPIRIT Checklist HYSTER. SPIRIT Checklist HYSTER shows the recommended items to address in a clinical trial protocol with corresponding page numbers. (DOC 122 kb
