さいたま市における小中一貫教育の現状と課題 : 技術・家庭科教育の観点から<人文・社会科学>

This research reveals the effect and issues on teaching from the survey of how teachers of technology education and home economics teach in their elementary schools which carry out the educational continuity from primary through early secondary levels. We picked up on Saitama City as the advanced local government promoting the system and surveyed the opinions of teachers who doubled as the elementary school and junior high school teacher. As a result, the specific effect and issues were found in the peculiar effort of Saitama City. Through the survey, we found positive and negative opinions. Some people say that the system is effective for “Gap in first-year junior high school.” Others say that teachers take the heavy burden. In order to solve these problems, we need to get ready the system of doubling as the elementary school and junior high school teacher. For example, the review of working contents for doubling teachers between schools and taking time for meetings on advanced and the day. These effort will improve the educational continuity from primary through early secondary levels.textapplication/pdfdepartmental bulletin pape

Inclusive Measurement of the Photon Energy Spectrum in b→sγ Decays

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Time-Dependent CP-Violating Asymmetry in B^0→p^0γ Decays

We report the first measurement of CP-violation parameters in B^0 → p^0γ decays based on a data sample of 657×10^6B\bar{B} pairs collected with the Belle detector at the KEKB asymmetric-energy e^+e^- collider. We obtain the time-dependent and direct CP-violating parameters, S_p0γ=-0.83±0.65(stat)±0.18(syst) and A_poγ= -0.44±0.49(stat)±0.14(syst), respectively.journal articl

国立大学機器・分析センターのこれから : 全国立大学の動きと本センターの方向と改革

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研究解説 : 平均場ダイナモ理論から見た太陽磁場の反転機構

departmental bulletin pape

Small bandgap in atomically precise 17-atom-wide armchair-edged graphene nanoribbons

Bottom-up synthesis of graphene nanoribbons (GNRs) may open new possibilities in future electronic devices owing to their tunable electronic structure, which depends strongly on their well-defined width and edge geometry. For instance, armchair-edged GNRs (AGNRs) exhibit width-dependent bandgaps. However, the bandgaps of AGNRs synthesized experimentally so far are relatively large, well above 1?eV. Such a large bandgap may deteriorate device performance due to large Schottky barriers and carrier effective masses. Here, we describe the bottom-up synthesis of AGNRs with smaller bandgaps, using dibromobenzene-based precursors. Two types of AGNRs with different widths, namely 17 and 13 carbon atoms, were synthesized on Au(111), and their atomic and electronic structures were investigated by scanning probe microscopy and spectroscopy. We reveal that 17-AGNRs have the smallest bandgap, as well as the smallest electron/hole effective mass, among bottom-up AGNRs reported so far. The successful synthesis of 17-AGNRs is a significant step toward the development of GNR-based electronic devices.journal articl

Histone H4 N-Terminal Acetylation in Kasumi-1 Cells Treated with Depsipeptide Determined by Acetic Acid−Urea Polyacrylamide Gel Electrophoresis, Amino Acid Coded Mass Tagging, and Mass Spectrometry

Disrupted patterns of acetylation and deacetylation of core histones play an important role in silencing transcription of hematopoietic important genes in acute myeloid leukemia (AML). A thorough investigation of these mechanisms and the response to pharmacologic modifiers will provide a better understanding of the role of histone acetylation in leukemogenesis. We describe here an analytical approach that combines acid urea polyacrylamide gel electrophoresis (AU-PAGE), amino acid coded mass tagging (AACM), and mass spectrometry (MS) for the investigation of histone acetylation patterns. The combined approach was used to follow the dynamics of H4 acetylation in Kasumi-1 cells harboring the fusion gene AML1/ETO shown to aberrantly recruit histone deacetylases (HDACs). The histones in Kasumi-1 cells were labeled by growing the cells in media in which lysine was replaced with stable isotope-labeled lysine (Lys-D4). Labeled and unlabeled cells were treated with depsipeptide and analyzed at different time points (0, 4, 8, 12, 24, and 48 h). The cells were mixed, the histone was extracted, and acetylated H4 isoforms were separated using AU-PAGE before in-gel trypsin digestion. The digests were analyzed by MALDI-TOF MS. Peptides were identified by mass and isotope pattern. LC−MS/MS of Arg-C digests were also performed to verify the acetylation pattern for H4. The major pattern of acetylation was determined as follows:  initial acetylation at K16, followed by acetylation at K12, and finally acetylation of either K8 and/or K5. Keywords: histone post-translational modification • mass spectrometry • Lys-D4 • isotope-labeled mass ta

Histone H4 N-Terminal Acetylation in Kasumi-1 Cells Treated with Depsipeptide Determined by Acetic Acid−Urea Polyacrylamide Gel Electrophoresis, Amino Acid Coded Mass Tagging, and Mass Spectrometry

Disrupted patterns of acetylation and deacetylation of core histones play an important role in silencing transcription of hematopoietic important genes in acute myeloid leukemia (AML). A thorough investigation of these mechanisms and the response to pharmacologic modifiers will provide a better understanding of the role of histone acetylation in leukemogenesis. We describe here an analytical approach that combines acid urea polyacrylamide gel electrophoresis (AU-PAGE), amino acid coded mass tagging (AACM), and mass spectrometry (MS) for the investigation of histone acetylation patterns. The combined approach was used to follow the dynamics of H4 acetylation in Kasumi-1 cells harboring the fusion gene AML1/ETO shown to aberrantly recruit histone deacetylases (HDACs). The histones in Kasumi-1 cells were labeled by growing the cells in media in which lysine was replaced with stable isotope-labeled lysine (Lys-D4). Labeled and unlabeled cells were treated with depsipeptide and analyzed at different time points (0, 4, 8, 12, 24, and 48 h). The cells were mixed, the histone was extracted, and acetylated H4 isoforms were separated using AU-PAGE before in-gel trypsin digestion. The digests were analyzed by MALDI-TOF MS. Peptides were identified by mass and isotope pattern. LC−MS/MS of Arg-C digests were also performed to verify the acetylation pattern for H4. The major pattern of acetylation was determined as follows:  initial acetylation at K16, followed by acetylation at K12, and finally acetylation of either K8 and/or K5. Keywords: histone post-translational modification • mass spectrometry • Lys-D4 • isotope-labeled mass ta

Histone H4 N-Terminal Acetylation in Kasumi-1 Cells Treated with Depsipeptide Determined by Acetic Acid−Urea Polyacrylamide Gel Electrophoresis, Amino Acid Coded Mass Tagging, and Mass Spectrometry

Disrupted patterns of acetylation and deacetylation of core histones play an important role in silencing transcription of hematopoietic important genes in acute myeloid leukemia (AML). A thorough investigation of these mechanisms and the response to pharmacologic modifiers will provide a better understanding of the role of histone acetylation in leukemogenesis. We describe here an analytical approach that combines acid urea polyacrylamide gel electrophoresis (AU-PAGE), amino acid coded mass tagging (AACM), and mass spectrometry (MS) for the investigation of histone acetylation patterns. The combined approach was used to follow the dynamics of H4 acetylation in Kasumi-1 cells harboring the fusion gene AML1/ETO shown to aberrantly recruit histone deacetylases (HDACs). The histones in Kasumi-1 cells were labeled by growing the cells in media in which lysine was replaced with stable isotope-labeled lysine (Lys-D4). Labeled and unlabeled cells were treated with depsipeptide and analyzed at different time points (0, 4, 8, 12, 24, and 48 h). The cells were mixed, the histone was extracted, and acetylated H4 isoforms were separated using AU-PAGE before in-gel trypsin digestion. The digests were analyzed by MALDI-TOF MS. Peptides were identified by mass and isotope pattern. LC−MS/MS of Arg-C digests were also performed to verify the acetylation pattern for H4. The major pattern of acetylation was determined as follows:  initial acetylation at K16, followed by acetylation at K12, and finally acetylation of either K8 and/or K5. Keywords: histone post-translational modification • mass spectrometry • Lys-D4 • isotope-labeled mass ta

The extracted ion chromatogram (XIC) for 5 mdC at / 242/126, 2dG at / 268/152 and () decitabine at 229/113 in Mobile Phase A spiked with 1 ng/ml 5 mdC, 1000 ng/ml of the 2dG and 200 ng/ml decitabine, respectively

<p><b>Copyright information:</b></p><p>Taken from "Characterization of and hypomethylating effects of decitabine in acute myeloid leukemia by a rapid, specific and sensitive LC-MS/MS method"</p><p></p><p>Nucleic Acids Research 2007;35(5):e31-e31.</p><p>Published online 30 Jan 2007</p><p>PMCID:PMC1865075.</p><p>© 2007 The Author(s).</p