さいたま市における小中一貫教育の現状と課題 : 技術・家庭科教育の観点から<人文・社会科学>

This research reveals the effect and issues on teaching from the survey of how teachers of technology education and home economics teach in their elementary schools which carry out the educational continuity from primary through early secondary levels. We picked up on Saitama City as the advanced local government promoting the system and surveyed the opinions of teachers who doubled as the elementary school and junior high school teacher. As a result, the specific effect and issues were found in the peculiar effort of Saitama City. Through the survey, we found positive and negative opinions. Some people say that the system is effective for “Gap in first-year junior high school.” Others say that teachers take the heavy burden. In order to solve these problems, we need to get ready the system of doubling as the elementary school and junior high school teacher. For example, the review of working contents for doubling teachers between schools and taking time for meetings on advanced and the day. These effort will improve the educational continuity from primary through early secondary levels.textapplication/pdfdepartmental bulletin pape

Inclusive Measurement of the Photon Energy Spectrum in b→sγ Decays

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Time-Dependent CP-Violating Asymmetry in B^0→p^0γ Decays

We report the first measurement of CP-violation parameters in B^0 → p^0γ decays based on a data sample of 657×10^6B\bar{B} pairs collected with the Belle detector at the KEKB asymmetric-energy e^+e^- collider. We obtain the time-dependent and direct CP-violating parameters, S_p0γ=-0.83±0.65(stat)±0.18(syst) and A_poγ= -0.44±0.49(stat)±0.14(syst), respectively.journal articl

「YNUテクノワールド2003」の実施を終えて

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Novel targets of ΔNp63 in keratinocytes identified by a modified chromatin immunoprecipitation approach-4

<p><b>Copyright information:</b></p><p>Taken from "Novel targets of ΔNp63 in keratinocytes identified by a modified chromatin immunoprecipitation approach"</p><p>http://www.biomedcentral.com/1471-2199/8/43</p><p>BMC Molecular Biology 2007;8():43-43.</p><p>Published online 23 May 2007</p><p>PMCID:PMC1890296.</p><p></p>rious ChIPed DNA sequences corresponding to known genes as indicated above each lane. Two radiolabeled oligonucleotide probes from each ChIPed sequence were each incubated with recombinant His-tagged ΔNp63α protein. Arrow indicates ΔNp63α. (B) EMSA was performed with nuclear extract from HaCaT cells and incubated with the radiolabeled oligonucleotide probe that bound best to recombinant protein demonstrated in A above. A complex containing ΔNp63 is supershifted with an anti-ΔNp63 antibody. See Additional File for oligonucleotide sequences used in EMSA

Novel targets of ΔNp63 in keratinocytes identified by a modified chromatin immunoprecipitation approach-0

<p><b>Copyright information:</b></p><p>Taken from "Novel targets of ΔNp63 in keratinocytes identified by a modified chromatin immunoprecipitation approach"</p><p>http://www.biomedcentral.com/1471-2199/8/43</p><p>BMC Molecular Biology 2007;8():43-43.</p><p>Published online 23 May 2007</p><p>PMCID:PMC1890296.</p><p></p>ers and amplified by PCR using the linker sequence for priming. The PCR-amplified products were subsequently incubated with agarose beads containing GST-ΔNp63 protein. ChIPed fragments were then digested with Hind III, cloned, and sequenced

Novel targets of ΔNp63 in keratinocytes identified by a modified chromatin immunoprecipitation approach-5

<p><b>Copyright information:</b></p><p>Taken from "Novel targets of ΔNp63 in keratinocytes identified by a modified chromatin immunoprecipitation approach"</p><p>http://www.biomedcentral.com/1471-2199/8/43</p><p>BMC Molecular Biology 2007;8():43-43.</p><p>Published online 23 May 2007</p><p>PMCID:PMC1890296.</p><p></p>omoter demonstrate high levels of activity when co-transfected with an expression plasmid encoding ΔNp63α in PtK2 cells. Luciferase values were determined and normalized against β-galactosidase values. Corrected luciferase values for cells transfected with empty vector pCMV-HA were set at 1. TK; thymidine kinas

Novel targets of ΔNp63 in keratinocytes identified by a modified chromatin immunoprecipitation approach-6

<p><b>Copyright information:</b></p><p>Taken from "Novel targets of ΔNp63 in keratinocytes identified by a modified chromatin immunoprecipitation approach"</p><p>http://www.biomedcentral.com/1471-2199/8/43</p><p>BMC Molecular Biology 2007;8():43-43.</p><p>Published online 23 May 2007</p><p>PMCID:PMC1890296.</p><p></p>ck-transfected cells were used as a control. Primer sequences used are listed in Additional File

Novel targets of ΔNp63 in keratinocytes identified by a modified chromatin immunoprecipitation approach-3

<p><b>Copyright information:</b></p><p>Taken from "Novel targets of ΔNp63 in keratinocytes identified by a modified chromatin immunoprecipitation approach"</p><p>http://www.biomedcentral.com/1471-2199/8/43</p><p>BMC Molecular Biology 2007;8():43-43.</p><p>Published online 23 May 2007</p><p>PMCID:PMC1890296.</p><p></p>ichment of target genes relative to IgG on an agarose gel. (B) Real-time PCR demonstrating the relative enrichment of p63 target genes with p63 antibodies and IgG. Primer sequences utilized are listed in Additional File

Novel targets of ΔNp63 in keratinocytes identified by a modified chromatin immunoprecipitation approach-2

<p><b>Copyright information:</b></p><p>Taken from "Novel targets of ΔNp63 in keratinocytes identified by a modified chromatin immunoprecipitation approach"</p><p>http://www.biomedcentral.com/1471-2199/8/43</p><p>BMC Molecular Biology 2007;8():43-43.</p><p>Published online 23 May 2007</p><p>PMCID:PMC1890296.</p><p></p>in Additional File to specifically amplify the target genes. β-Actin serves as a control