A Study on the Improvement about Teaching Method and Evaluating Method in Lower Secondary School Science - Focused on the Light Conception in Lower Secondary Level -

departmental bulletin pape

Search for B decays into η'p, η'K^*, η'φ, η'ω and η'η^(')

We report on a search for the exclusive two-body charmless hadronic B meson decays B → η'ρ, B → η'K^*, B^0 → ηφ, B^0 → η'ω, and B0 →η'η^('). The results are obtained from a data sample containing 535×10^6 B\bar{B} pairs that were collected at the Υ(4S) resonance with the Belle detector at the KEKB asymmetric-energy e^+e^- collider. We find no significant signals and report upper limits in the range (0.5–6.5)×10^-6 for all of the above decays.journal articl

Observation of Large CP Violation and Evidence for Direct CP Violation in B0→π+π- Decays

journal articl

The vertical line indicates the actual position of the simulated gene and horizontal lines correspond to the threshold values given by one--score drop from the peak of the curves

<p><b>Copyright information:</b></p><p>Taken from "Linkage analysis of complex diseases using microsatellites and single-nucleotide polymorphisms: application to alcoholism"</p><p></p><p>BMC Genetics 2005;6(Suppl 1):S10-S10.</p><p>Published online 30 Dec 2005</p><p>PMCID:PMC1866840.</p><p></p> The -values corresponding to the highest peaks are 0.005 for microsatellites and 2 × 10to 7 × 10for different panels of SNPs

研究速報 : 固気混相乱流のTwo Way Coupling LESにおけるSGSモデルの改良

departmental bulletin pape

Supplemental Material - Utilising high-dimensional data in randomised clinical trials: A review of methods and practice

Supplemental Material for Utilising high-dimensional data in randomised clinical trials: A review of methods and practice by Svetlana Cherlin, Theophile Bigirumurame, Michael J Grayling, Jérémie Nsengimana, Luke Ouma, Aida Santaolalla, Fang Wan, S Faye Williamson and James MS Wason in Research Methods in Medicine & Health Sciences.</p

Additional file 1 of ROR2 has a protective role in melanoma by inhibiting Akt activity, cell-cycle progression, and proliferation

Additional file 1: List of antibodies used

Additional file 2 of ROR2 has a protective role in melanoma by inhibiting Akt activity, cell-cycle progression, and proliferation

Additional file 2: Figure S1. (A) ROR2 inhibits cell growth. Crystal violet assays were performed in UACC903 cells stably transduced with either control (Empty) or a ROR2-expressing plasmid. Levels of ROR2 in these cells were determined by western blot. The analysis was performed as described for A375 in Fig. 1B. (B, C) Flow cytometry analysis of ROR2 levels in A375 and UACC903 stably transduced with either control (Empty) or a ROR2-expressing plasmid. The experiment was performed with either permeabilized (B) or intact (C) cells. Cells were collected and stained as described in “Methods”. Light-grey filled histogram corresponds to control (isotype) antibody and open histograms to ROR2 antibody. Bar graph shows the mean of ROR2 MFI (Mean Fluorescence Intensity) ± S.D. (from three independent experiments). Statistical significance was tested by a one-tailed Student’s T-Test, n = 3. The histograms displayed are representative of three independent experiments. (D) ROR2 levels upon overexpression are similar to those found in HeLa cells. ROR2 levels were assessed by western blot in HeLa, M2, A375-Empty, and A375-ROR2 cells. Bar graph shows the mean ± S.D. (from three independent experiments) of ROR2 levels normalized to the loading control. The blots displayed are representative of three independent experiments. Statistical significance was tested by ANOVA, n = 3. (E) ROR2 silencing increases cell growth. Crystal violet assays were performed in M2 cells upon silencing of ROR2. Levels of ROR2 in these cells were determined by western blot. The analysis was performed as described in Fig. 1B. (F) Efficient silencing of ROR2 in M2 and MeWo cells. Flow cytometry analysis of ROR2 levels in M2 and MeWo stably transduced with either control (scramble) plasmid or two shRNA for ROR2 (C2 and C4). Light-grey filled histogram corresponds to control (isotype) antibody and open histograms to ROR2 antibody. Bar graph shows the mean of ROR2 MFI (Mean Fluorescence Intensity) ± S.D. (from three independent experiments). Statistical significance was tested by a one-tailed Student’s T-Test (M2) or ANOVA (MeWo), n = 3. The histograms displayed are representative of three independent experiments. (G, H) ROR2 impairs cell-cycle progression. Flow cytometry analysis of PI-stained UACC903 cells overexpressing ROR2 (G) and M2 (H) cells upon silencing of ROR2. Bar graph shows the mean ± S.D. of the percentage of cells in each phase of the cell-cycle. The analysis was performed as described in Fig. 1E and F. *: p < 0.01, **: p < 0.001, ***: p < 0.0001, n = 3. n.s.: not significant. Figure S2. (A) LY294002 similarly inhibits p-Akt levels in both M2-scramble and M2-shROR2 cells. M2-scramble and M2-shROR2 cells were incubated with LY for the indicated times and protein extracts were analyzed by western blot with the indicated antibodies. GAPDH was used as loading control. Analysis was performed as described in Fig. 3A. (B) LY294002 decreases the viability of M2-shROR2 cells. M2-scramble and M2-shROR2 cells were incubated with the indicated concentrations of LY294002 for 48 h. Graphs show the mean ± S.D. of the percentage of viable cells. Statistical significance was tested by a one-tailed Student’s T-Test, n = 3. (C) Inhibition of Akt signaling does not induce apoptosis in cells with ROR2 knockdown. M2 and MeWo cells stably transduced with either control (scramble) plasmid or shRNA for ROR2 (C4 and C2) were incubated with 20 μM LY294002 for 48h. Cells were stained with Annexin V/Propidium Iodide and analyzed by flow cytometry. The dot plots displayed are representative of three independent experiments. The percent of cells in each quadrant is indicated. *: p < 0.01, **: p < 0.001, ***: p < 0.0001, n.s.: not significant

Legend for Supplementary tables from Vitamin D–VDR Signaling Inhibits Wnt/β-Catenin–Mediated Melanoma Progression and Promotes Antitumor Immunity

Legend for Supplementary tables</p

Supplementary Figure 4 from Vitamin D–VDR Signaling Inhibits Wnt/β-Catenin–Mediated Melanoma Progression and Promotes Antitumor Immunity

Comparison of CD3 positive immune infiltrate in murine lungs</p