通信装置の高速・高密度実装技術に関する研究

名古屋大学Nagoya University博士(工学)名古屋大学博士学位論文 学位の種類:博士(工学) (論文) 学位授与年月日:平成8年12月9日doctoral thesi

特許の活用，未活用の要因に関する研究

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Upper Bound on the Decay τ→μγ from the Belle Detector

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FOUR VERTICES THEOREMS FOR SURFACE CURVES AND SPACE CURVES.

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Expression patterns of <i>suffix</i> and F elements in <i>Drosophila menalogaster</i> ovaries.

<p><i>In situ</i> hybridizations with DIG-labeled, strand-specific RNA probes were performed (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000476#s4" target="_blank">Materials Methods</a>). (A, B) The patterns revealed by <i>suffix</i> sense and antisense probes, respectively. (C, D) The patterns revealed by F element sense and antisense probes, respectively. Black arrows indicate the transcripts detected in the cytoplasm of nurse cells. White arrows denote F element transcripts in follicle cells.</p

Relationship between the <i>Drosophila</i> F element and <i>suffix.</i>

<p>Neighboring DNA fragments (shown in brackets) were used for the preparation of both <i>suffix</i>-and F element-specific RNA probes for Northern analysis.</p

Northern blot analysis of <i>suffix</i>-specific transcripts.

<p>Hybridizations were performed with <i>suffix</i>-specific antisense (as) or sense (s) [³²P]-labeled RNA probes. The lanes with poly(A)⁺ and poly(A)⁻ RNA samples, isolated from <i>Drosophila</i> embryos (E), larvae (L), pupae (P) and imago (I) are shown. Positions of the RNA markers in nt are shown on the right. The band of around 300 nt in length is shown on the left. Quantitation of the RNA content by hybridization with an rp49-specific probe is shown at the bottom.</p

Expression patterns of <i>suffix</i> and F elements in <i>Drosophila</i> testis.

<p><i>In situ</i> hybridizations with DIG-labeled, strand-specific RNA probes were performed (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000476#s4" target="_blank">Materials Methods</a>). (A, B) The patterns revealed by <i>suffix</i> sense and antisense probes, respectively. (C, D) The patterns revealed by F element sense and antisense probes, respectively. Arrows indicate the transcripts detected in primary spermatocytes.</p

Cloning of F element transcripts lacking <i>suffix</i> sequences using 3′RACE.

<p>(A) Schematic illustration of the procedures used (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000476#s4" target="_blank">Materials and Methods</a>). After the addition of a poly(A) tail to the poly(A)⁻RNA preparations isolated from pupae or ovaries, the samples were used for reverse transcription with a poly(T) primer and subsequent PCR using poly(T)-and F element-specific primers. (B) The number of clones identified by hybridization with <i>suffix</i>-specific or F-element-specific probes in colony-hybridization experiments. Five clones from pupae (3, 5, 16, 28 and 38) showed hybridization with the F-element probe only were selected for sequencing. Similarly, two clones (15 and 55) were isolated from the wild type ovaries. (C) The sequences of the cDNA clones isolated from pupae or ovaries that were truncated by RNA silencing mechanisms at the very beginning of the <i>suffix</i> sequence (shown in bold) are presented (clones 38 and 28 are identical by sequence to clones 5 and 16, respectively). (D) Diagram showing the positions of the cut sites at the very beginning of the <i>suffix</i> sequence in the cDNA clones corresponding to F element transcripts, indicated by arrows.</p

Northern blot analysis of F element-specific transcripts.

<p>Hybridizations were performed with F element-specific antisense or sense [³²P]-labeled RNA probes. The labeling is as described for <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000476#pone-0000476-g002" target="_blank"><u>Figure 2</u></a>.</p