SiCウイスカ-砥石の開発

名古屋大学Nagoya University博士(工学)名古屋大学博士学位論文 学位の種類:博士(工学) (論文) 学位授与年月日:平成10年1月6日doctoral thesi

離職者訓練（委託訓練）における職業訓練効果の一考察 : 職業訓練講師の意識と訓練生への働きかけを中心にして

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Dendrobium Swarz.(ラン科)の類縁に関する研究 : I. Eugenanthe Schlechter節内での交配親和性

1.ノビルタイプのデンドロビウム品種に.新しい遺伝子を導入する可能性を調べるため, Eugenanthe節内の22種と、D. moniliforme(セッコク), D. nobileとの交配を行なった.2.交配稔性からみて, 本節内にはD. moniliforme, D. nobileとは遠縁と思われる種が含まれていた.3.D. moniliformeは, D. nobileに比べ, 多くの種と交雑可能で, 今後の育種のために有用な種と考えられた.In order to check the possibility of introducing new genes into the modern nobile-type cultivars of Dendrobium, D. nobile Lindl. and D. moniliforme (L.) Swarz. were crossed with selected species of section Eugenanthe Schlechter. D. moniliforme showed a wider range of crossability with Eugenanthe species compared to D. nobile. Eugenanthe species were divided into two groups according to their crossability with D. moniliforme

Upper Bound on the Decay τ→μγ from the Belle Detector

journal articl

ADJUSTED RELATIVITY THEORY : APPLICATIONS OF EXTENDED EUCLIDEAN GEOMETRY, EXTENDED EQUIFORM GEOMETRY AND EXTENDED LAGUERRE GEOMETRY TO PHYSICS.

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SARS-CoV 3b interacts with RUNX1b.

<p>A. A schematic representation of full-length 3b, pHybLexA/Zeo-3b bait plasmid, full-length RUNX1b and pYesTrp2-RUNX1b (51–421 aa) prey plasmid. The 3b protein has a nucleolar localization signal (NoLS) at the C-terminus. The RUNX1b protein has a runt homology domain (RHD, 50–177 aa), an activation domain (AD, 291–171 aa), and an inhibitory domain (ID, 346–411 aa). B. The 3b-RUNX1b interaction was assessed on a selective growth media (supplemented with 3-AT) and by filter lift β-galactosidase activity assay in a yeast two-hybrid experiment. pHybLexA/Zeo, pYesTrp2, pHybLexA/Zeo-3b, pYesTrp2-RUNX1b, pHybLexA/Zeo-Fos and pYesTrp2-Jun constructs were co-transformed in L40 in combinations tabulated above. pHybLexA/Zeo-Fos and pYesTrp2-Jun were used as positive control. C, D. <i>In vitro</i> analysis of 3b and RUNX1b interaction. C. <i>In vitro</i> translated S^35-labelled 3b and RUNX1b lysates (input) were subjected to co-immunoprecipitation alone or together, using α–RUNX1 antibody. D. Total cell lysates of Huh7 cells expressing indicated proteins were immunoprecipitated with α-Flag antibody and western blotted with α-myc antibody to probe 3b protein (panel 1). Lysates were probed for the expression of RUNX1b and 3b with α-Flag and α-myc antibodies, respectively.</p

3b recruitment on RUNX1 binding elements on the IL2 promoter.

<p>Chromatin immunoprecipitation assays were performed with jurkat cells transfected with vector alone or HA-3b using α-RUNX1 and α-HA antibodies. PCR amplifications were performed using IL2 promoter primers and 3′ distal IL2 promoter primers. Results are representative of three independent experiments.</p

3b expression increases phosphorylated RUNX1b levels through ERK activation.

<p>A. HEK293 cells were transfected with vector, 3b and RUNX1b expression plasmids. ERK immunoprecipitated from vector and 3b lysates were subjected to kinase assay with RUNX1b beads. Phosphorylated RUNX1b was visualized by autoradiography. Input levels of immunoprecipitated ERK and phospho ERK levels in lysates were probed by western blotting. Graph depicts fold increase in the levels of phosphorylated RUNX1b procured after three independent experiments. Bar represents mean±SD of values obtained by densitometry. #, <i>p</i><0.05. B. Jurkat cells were transfected with WT IL2-Luc in the presence or absence of Myc-3b and treated with DMSO or U0126. Relative luciferase activity was measured and is shown as the mean±SD of three independent experiments performed in triplicates. *, <i>p</i><0.005. Phospho ERK levels in lysates were probed by western blotting.</p

3b increases RUNX1b transactivation potential on the mouse IL2 promoter.

<p>A. HEK293 cells were transfected with WT IL2-Luc plasmid alone or with Flag-RUNX1b, Flag-CBFβ and indicated amounts of Myc-3b. Relative luciferase activities were calculated 48 h post transfection. B. Jurkat cells were transfected with WT IL2-Luc and indicated amounts of Myc-3b. Relative luciferase activities were calculated 48 h post-transfection. 3b expression was probed using α-myc antibody C. Jurkat cells were transfected with WT IL2-Luc or mutant IL2-Luc plasmid in the presence or absence of Myc-3b. Results in each panel are represented as mean±S.D. of triplicate cultures. Bar values represent fold increase in luciferase activity. *, <i>p</i><0.005.</p

3b partially co-localizes with RUNX1b in the nucleus.

<p>Cellular distribution of 3b and RUNX1b proteins were visualized by subjecting Flag-RUNX1b and HA-3b transfected HEK293 cells to immunofluorescence assay. 3b was visualized using primary α–HA and alexa-488 conjugated secondary antibody. RUNX1b was visualized using primary α–RUNX1 and alexa-594 conjugated secondary antibody. Nuclei were visualized by DAPI (4′6-diamidino-2-phenylindole) staining. Arrows indicate extra nucleolar nucleus area of partial co-localization.</p