Development of an Ion Stacking Method by Aqueous Partition Chromatography with a Hydrophilic Polymer Gel Column

学位記号番号 : 博理工甲第1084号博士の専攻分野の名称 : 博士(工学) 学位授与年月日 : 平成30年3月23日textapplication/pdfthesi

Upper Bound on the Decay τ→μγ from the Belle Detector

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ON EXISTENCE OF SOME SUBALGEBRA OF A GIVEN BOOLEAN ALGEBRA WHICH IS COUNTABLY INFINITE.

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The Establishment of the National Language in 20th Century Cambodia: Debates on Orthography and Coinage (Video)

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研究速報 : 生成された変動風を流入境界条件とする2次元角柱周辺流れのLES

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Tumor incidence, mammary gland alterations and body weight curves in MPA-treated mice

<p><b>Copyright information:</b></p><p>Taken from "Association of estrogen receptor-α and progesterone receptor A expression with hormonal mammary carcinogenesis: role of the host microenvironment"</p><p>http://breast-cancer-research.com/content/9/2/R22</p><p>Breast Cancer Research 2007;9(2):R22-R22.</p><p>Published online 6 Mar 2007</p><p>PMCID:PMC1868922.</p><p></p> (a) Mammary tumor incidence. C57BL/6 mice were implanted with 40 mg MPA pellets that were replaced 6 months later; historical values of BALB/c using the same protocol were included for comparison. No mammary tumors appeared in MPA-treated or control C57BL/6 mice; control mice were implanted subcutaneously with silastic pellets. Mammary gland alterations in MPA-treated BALB/c and C57BL/6 mice after one year of MPA treatment. The subpanels show the following: (a) a distorted and rotated ductal structure that appears to be forcibly stretched in areas (WM); (b) hyperplasia in the form of irregular paraductal proliferation (WM); (c) atypical ductal side branching showing areas of neoplastic growth (arrows; WM); (d) hematoxylin and eosin showing ductal hyperplasia; (e,f) WM and hematoxylin and eosin pictures showing nearly normal mammary glands of MPA-treated C57BL/6 mice; and (g,h) WM and hematoxylin and eosin pictures of C57BL/6 control mice. Body weight curves of C57BL/6 mice implanted with 40 mg MPA (first arrow) or control blank silastic pellets that were replaced after 6 months (second arrow). All animals were weighed weekly and the results expressed as mean ± standard error. MPA-treated animals showed an increase in body weight that was already evident after the second week of treatment (< 0.001; two-way ANOVA). ANOVA analysis of variance; MPA, medroxyprogesterone acetate; WM, whole mount

PR expression in mammary glands treated with progestins for 2 months: immunohistochemical studies

<p><b>Copyright information:</b></p><p>Taken from "Association of estrogen receptor-α and progesterone receptor A expression with hormonal mammary carcinogenesis: role of the host microenvironment"</p><p>http://breast-cancer-research.com/content/9/2/R22</p><p>Breast Cancer Research 2007;9(2):R22-R22.</p><p>Published online 6 Mar 2007</p><p>PMCID:PMC1868922.</p><p></p> Control mice received blank silastic pellets. For both PR-A and PR-B, a representative image of four to six histologic slides per group is shown. The number of stained cells is expressed as the percentage of the number of stained cells per epithelial cells in 400× fields of every slide and a minimum of 1,500 epithelial cells/slide were counted (bar: 80 μm). The right panels show the quantification in control (filled columns), MPA-treated (dotted columns), and Pg-treated (empty columns) mice (mean ± standard error). PR-A expression decreased significantly in progestin-treated BALB/c mice, whereas PR-B expression increased in progestin-treated mice of both strains. *< 0.05, **< 0.01, ***< 0.001 control versus treated mice. Quantification (mean ± standard error) of the percentage of positive stained cells for ER-α, ER-β, PR-A, and PR-B in control BALB/c or C57BL/6 female mice shown in Figures 3 and 4a,b. BALB/c mice show higher levels of ER-α and PR-A than C57BL/6 female mice. In control groups, animals were killed at diestrus. ***< 0.001, **< 0.01. ER, estrogen receptor; MPA, medroxyprogesterone acetate; Pg, progesterone; PR, progesterone receptor

Morphology of reconstituted mammary glands of both strains into a background

<p><b>Copyright information:</b></p><p>Taken from "Association of estrogen receptor-α and progesterone receptor A expression with hormonal mammary carcinogenesis: role of the host microenvironment"</p><p>http://breast-cancer-research.com/content/9/2/R22</p><p>Breast Cancer Research 2007;9(2):R22-R22.</p><p>Published online 6 Mar 2007</p><p>PMCID:PMC1868922.</p><p></p> Mammary cells were isolated from 2-month-old BALB/c or C57BL/6 mice and 5 μl of cell suspensions were transplanted into the right or left cleared mammary fat pads, respectively, of 21-day-old Swiss or syngeneic mice. At surgery pellets containing Eplus Pg or plus MPA were also implanted subcutneously into the backs of the animals. One month later, animals were killed and mammary glands excised and fixed. Microscopic examination revealed no changes between C57BL/6 or BALB/c repopulated Swiss /glands, whereas C57BL/6 cells transplanted in syngeneic glands were under-developed (100×, bar: 700 μm). A representative image of the WM or the H&E stained slides of the reconstituted mammary glands in E/Pg-treated mice is shown. Microsatellite studies confirmed the strain origin of the epithelial cells in the Swiss background. bp, base pairs; E, 17β-estradiol; H&E, hematoxylin and eosin; LCM, laser capture microdissection; MPA, medroxyprogesterone acetate; Pg, progesterone; WM, whole mount

Effects of progestins in salivary gland morphology and in serum pituitary hormone levels

<p><b>Copyright information:</b></p><p>Taken from "Association of estrogen receptor-α and progesterone receptor A expression with hormonal mammary carcinogenesis: role of the host microenvironment"</p><p>http://breast-cancer-research.com/content/9/2/R22</p><p>Breast Cancer Research 2007;9(2):R22-R22.</p><p>Published online 6 Mar 2007</p><p>PMCID:PMC1868922.</p><p></p> Salivary glands from control, MPA, or Pg-treated BALB/c or C57BL/6 mice. Pellets were implanted subcutaneously and the animals killed after 2 months. MPA increased the development of convoluted granular ducts (arrow). No differences were observed between both strains of mice (400×, bar: 120 μm). Serum PRL, Pg, GH, and IGF-I levels measured by radioimmunoassay in control BALB/c or C57BL/6 female mice and after 24 hours of MPA or Pg treatment. No differences were detected in C57BL/6 mice as compared with BALB/c mice. Furthermore, Pg and MPA treatment induced an increase in PRL levels in both strains (< 0.001, two-way ANOVA). The increase in Pg levels after progesterone administration was also similar in both strains (< 0.001, two-way ANOVA). Gray bars: BALB/c; black bars: C57BL/6. ANOVA, analysis of variance; GH, growth hormone; IGF, insulin-like growth factor; MPA, medroxyprogesterone acetate; Pg, progesterone; PR, progesterone receptor; PRL, prolactin

PR expression in mammary glands treated with progestins for 2 months: Western blot studies

<p><b>Copyright information:</b></p><p>Taken from "Association of estrogen receptor-α and progesterone receptor A expression with hormonal mammary carcinogenesis: role of the host microenvironment"</p><p>http://breast-cancer-research.com/content/9/2/R22</p><p>Breast Cancer Research 2007;9(2):R22-R22.</p><p>Published online 6 Mar 2007</p><p>PMCID:PMC1868922.</p><p></p> Mammary glands from the same groups shown in Figure 4 were processed to obtain nuclear extracts (as explained in Materials and methods) and used in Western blots. A representative experiment using C-19 PR Santa Cruz antibody is shown. Because different amounts of epithelial cells are present in the different extracts, the ratio of PR-A/PR-B in three different experiments is shown. *< 0.05, **< 0.01, ***< 0.001 treated versus control. PR, progesterone receptor