Condensate Formation by Metabolic Enzymes in Saccharomyces cerevisiae

application/pdfMicroorganisms. 2022, 10 (2), P.232journal articl

慢性疼痛における心理面のアセスメントについて : 心理社会的側面におけるスクリーニングおよび質問紙ツールの開発に向けての検討 [論文要旨及び審査の要旨]

関西大学博士（心理学）thesi

慢性疼痛における心理面のアセスメントについて : 心理社会的側面におけるスクリーニングおよび質問紙ツールの開発に向けての検討 [全文の要約]

関西大学博士（心理学）thesi

Change of the closeness between students by cross-age interaction in elementary school

departmental bulletin pape

Upper Bound on the Decay τ→μγ from the Belle Detector

journal articl

CERTAIN DOUBLE WHITTAKER TRANSFORMS OF GENERALIZED HYPERGEOMETRIC FUNCTIONS

application/pdfdepartmental bulletin pape

Overview of probe design: genomic coordination of TSSs and CAGE-TSSchip probes

<p><b>Copyright information:</b></p><p>Taken from "CAGE-TSSchip: promoter-based expression profiling using the 5'-leading label of capped transcripts"</p><p>http://genomebiology.com/2007/8/3/R42</p><p>Genome Biology 2007;8(3):R42-R42.</p><p>Published online 26 Mar 2007</p><p>PMCID:PMC1868931.</p><p></p> The upper four tracks are an arrangement example of full-length transcripts (cDNA) and 5'-ends of transcripts derived from various methods (cap analysis gene expression [CAGE], 5'-expressed sequence tag [EST], and 5'-end of gene identification signature/gene signature cloning [4]). Tag clusters (TC; green arrow) are the overlapping regions of the 5'-ends. The most frequent transciption start site (TSS) for each TC is the representative position (vertical line from TC arrows). Fragments for the probe design, of 120-nucleotide long genomic sequences, starts from the representative position of each TC fragment, shown by cyan arrows. If the fragment overlaps the 5'-end of any exon-intron junction (diamond of cDNA and 5'-EST transcripts), the fragment skips the intron to the next exon. According to the Agilent probe design service, the 60-nucleotide appropriate region within each fragment would then be suggested for array probes (probe; blue arrows). Details of probe preparation are available in Additional data file 8

Multifunctional Implantable Device for Simultaneous Optical and Electrophysiological Measurements

In neuroscience, it is crucial to clarify the relationship between single-cell activity and neural network structure to understand brain neural activity. To this end, a device that can measure signals with high temporal resolution over a wide area is required. In this study, we developed a multifunctional implantable device for measuring deep-brain functions. The device conducts electrophysiological measurements using microelectrodes and fluorescence imaging using a CMOS image sensor, which enables observations of brain functions with high temporal resolution over a wide area. The device developed is implemented by stacking the microelectrode array and imaging device. We measured the activity of neurons in the ventral tegmental area (VTA) of mice using this device. We successfully recorded action potentials and confirmed that the developed microelectrodes are effective for measuring brain functions. Results suggest that the multifunctional implantable device developed can simultaneously perform electrophysiological measurements and fluorescence imaging using a CMOS image sensor. However, the noise generated during imaging should be eliminated in the future. The activation of γ-aminobutyric acid neurons was also confirmed upon the intraperitoneal injection of nicotine solution as a pharmacological stimulus. The device developed with integrated microelectrodes and a CMOS image sensor is unprecedented and can prove useful in understanding the relationship between neuronal activity and neural networks.journal articl