質保証は絵空事か : 第２期認証評価実践上の課題

departmental bulletin pape

GUI の確立にみる「ディスプレイ行為」の形成過程

名古屋大学Nagoya University博士(情報科学)名古屋大学博士学位論文 学位の種類:博士（情報科学）（課程） 学位授与年月日:平成21年3月25日doctoral thesi

年代・エリアを限定したネット調査の課題 : １８・１９歳×中部エリアの事例〔論文〕

第24回参議院通常選挙の投票では、選挙権年齢が20歳から18歳にまで引き下げられた．メディアが利用するRDD調査では若い対象者からの回収が極めて悪いため、選挙情勢を見極める補足手段として、インターネット・パネルの若者（18～19歳）の選挙意識を調べた．早期回答よりは後期回答で、夜間回答よりも昼間回答で「投票に行った」割合が高いことなどが明らかになった．ただし、地方では18～19歳の若者を調査するのに十分な数のパネルが確保されていない．今後の利用のためには、若年層のパネル構築が必要とされる． The minimum voting age was revised from twenty to eighteen years for the 24th Upper House election. However, sample response rates for young persons through the Random Digit Dialing polls were very low; hence, we have used online panels for eighteen- to nineteen year-olds to predict and analyze election results. This article reports that the rate answered “I voted.” was higher in late respondents than early respondents, and similarly more people responded in the day voted than in the after-dark. However, we could not achieve a large-enough sample of eighteen- to nineteen-year-olds for analysis owing to the difficulty of reaching young respondents. We need to find ways to improve access to young people on databases in the online research industry.１．はじめに ２．調査設計 ３．準備段階 ４．実査段階 ５．課題点textapplication/pdfdepartmental bulletin pape

Factors Associated with Increased Caregiver Burden of Informal Caregivers during the COVID-19 Pandemic in Japan

application/pdfJournal of Nutrition, Health and Aging. 2022, 26 (2), P.157-160journal articl

Observation of B+→pp̅π+, B0→pp̅K0, and B+→pp̅K*+

journal articl

Corrosion Diagnosis Investigation of Archaeological Iron Objects in Exhibition Case of Tottori Mukibanda Yayoi Settlement Site

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CURVATURE TENSORS' AND THEIR RELATIVISTICS SIGNIFICANCE

application/pdfIn this Paper we have defined the Curvature tensor and elaborated its various Physical and geometric Properties.departmental bulletin pape

Characterization of scFvs Derived from Phage Libraries

<div><p>Estimation of diversity of library scFv inserts by BstNI digestion. Figure shows restriction digest patterns of 50 randomly selected scFvs from the October library.</p><p>(A) 50 inserts of the V_H–V_K scFvs amplified by PCR screen from 50 randomly selected colonies.</p><p>(B) The same V_H–V_K scFv inserts after digestion with BstNI. A large number of different digestion patterns are seen, suggesting a high degree of diversity among the library.</p><p>(C–E) Binding of polyclonal scFv from panning rounds 1–4 to P. falciparum by IFA [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.0030072#ppat-0030072-b020" target="_blank">20</a>]. IFA was carried out using acetone-fixed parasites, and the polyclonal scFv tested against the T9–96 and FCB-1 strains of P. falciparum at 20 μg/ml [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.0030072#ppat-0030072-b020" target="_blank">20</a>]. MAb 12.8 was used as a positive control and polyclonal scFv prepared from the unpanned libraries used as negative controls. Immunoblots of polyclonal scFv from fourth round of panning binding to P. falciparum strain T9/96 (D) or FCB-1 (E) merozoites [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.0030072#ppat-0030072-b020" target="_blank">20</a>]. Lane 1, scFv March library unpanned; lane 2, scFv October library unpanned; lane 3–6, scFv from fourth round of panning with unprocessed merozoites; lane 7–8, scFv from fourth round of panning with processed merozoites; lane 9, scFv from fourth round of panning with recombinant MSP1₁₉-GST; lane 10, scFv D1.3 (anti-hen's egg lysozyme); lane 11, scFv X509 (anti-MSP1₃₃); lane 12, scFv 89.1 (anti-MSP1₈₃); lane 13, mAb 12.8 (anti-MSP1₄₂ and MSP1₁₉).</p><p>(F) Inhibition of binding of mAbs 12.8 and 12.10 to MSP1₁₉ by scFvs derived from the fourth round of panning by competition ELISA [<a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.0030072#ppat-0030072-b020" target="_blank">20</a>].</p></div

Course of a P. falciparum MSP1₁₉ Transgenic P. berghei Infection in Mice

<div><p>(A) Groups of 2–3 FcγRI transgenic (Tg) or nontransgenic (NTg) littermates were injected i.p. with a total dose of 1.5 mg fully human anti–P. falciparum MSP1₁₉ IgG1 (JS1), an irrelevant human IgG1 (B10) recognizing MSP1₁₉ from P. yoelii or PBS. Similar results were obtained in two independent experiments. **Only groups of mice given JS1 in the FcγRI Tg were significantly different to all the other control groups with a <i>p</i> < 0.01. †, death of mice.</p><p>(B) Repeat experiment in FcγRI Tr animals using a lower total dose (0.75 mg) of JS1. Coadministration of the blocking mAb 10.1 specific for the IgG1 binding site on human FcγRI abrogates the protection mediated by the passively administered JS1 antibody. Each point represents the geometric mean parasitemia of mice in each group at the time after i.p. challenge with 5,000 parasitized erythrocytes. Only those animals receiving the fully human anti–P. falciparum MSP1₁₉ IgG1 Ab in a human FcγRI background survived an otherwise lethal infection; all the mice in the other groups with high parasitemias were killed on either day 7 or 8. Similar results were obtained in two independent experiments. **Only groups of mice given JS1 in the FcγRI Tg were significantly different to all the other control groups with a <i>p <</i> 0.01. †, death of mice.</p><p>(C–E) ×100 magnification of Giemsa-stained smears of blood taken from control animals (C) and FcγRI Tg animals treated with JS1 (D and E). Note the presence of phagocytosed merozoites within the cytoplasm of cells displaying mononuclear morphology (arrow).</p></div

Epitope Mapping of JS1 and JS2 Binding Sites

<p>Shows the location in the three-dimensional model of P. falciparum MSP1₁₉ of residues in the first epidermal growth factor domain, which on mutation affect binding by JS1 or JS2. Mutation of Cys²⁸ shown in red completely ablated binding of both mAbs (12.10 and 12.8) and JS1 or JS2. Mutation of the partnering Cys¹², also shown in red, while ablating binding by the murine mAbs, had no effect on the binding by JS1 or JS2. Arg²⁰ and Asn³³ in salmon had intermediate effects on binding as determined by SPR analysis when mutated to more neutral or negatively charged side-chains (see <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.0030072#ppat-0030072-t001" target="_blank">Table 1</a>). Three further substitutions at Lys⁴⁰, Lys²⁹, and Asn³⁹ seen in brown had minor effects on binding when the interaction was studied by ELISA. The model of P. falciparum MSP1₁₉ was generated by PyMol using atomic coordinates available from NCBI under accession number PDB: 1CEJ.</p