目的変数の合成に関する課題の考察 －病院における人事評価を例として

departmental bulletin pape

ICT Usage for Learning in Tottori prefecture - The Proposal and Vision by ICT Usage in Schools Promotion group -

departmental bulletin pape

Observation of the DsJ(2317) and DsJ(2457) in B Decays

journal articl

ON THE GROWTH OF COMPOSITE ENTIRE FUNCTIONS

application/pdfIn this paper, we study the order and growth of composite entire functions ＄f＄ and ＄g＄, and disscuss the behaviour as ＄r ￥rightarrow ￥infty＄ of the rations: ＄￥frac{￥log ￥log M(r,fog)}{￥log ￥log M(R,f)}＄ and ＄￥frac{￥log ￥log M(r,fog)}{￥log ￥log M(R,g)}, where ＄R=R(r)＄ is an increasing real function.departmental bulletin pape

0812-9869-9940 (WA), Jual Keranda Mayat Kalikudi

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海馬へのイボテン酸投与によるdark neuronの出現とその経過

(運動・神経生理)journal articl

Effect of <i>yidC</i> antisense expression on growth of <i>E. coli</i>.

<p><i>E. coli</i> DH5α harbouring pHN678 (negative control for antisense expression), pHN678-yidC or pHN678-murA (positive control for antisense expression) were cultured in LB broth supplemented with chloramphenicol (30 µg/ml). The overnight cultures were diluted hundred fold in fresh LB broth supplemented with chloramphenicol (30 µg/ml) of which 5 µl was spotted on LB chloramphenicol plates without (upper panel) and with IPTG (lower panel). There was no change observed in the phenotype of <i>E. coli</i> DH5α harbouring vector (pHN678) without any insert upon induction with IPTG after 14 hrs of incubation at 37°C. However, the growth of <i>E. coli</i> DH5α cells expressing antisense of <i>murA</i> and <i>yidC</i> showed growth suppression upon induction.</p

Effect of <i>yidC</i> antisense expression on corresponding gene transcript.

<p>Relative Real-time quantitative PCR (qPCR) was carried out to compare <i>yidC</i> mRNA expression in uninduced and induced pHN678-yidC harbouring <i>E. coli</i> DH5α cells. The CT values were averaged from triplicate samples and the expression ratio was calculated. Comparison of expression ratio of <i>yidC</i> gene upon its antisense induction in <i>E. coli</i> with different IPTG concentrations showed that <i>yidC</i> mRNA expression decreased with increasing IPTG concentration. However, the expression is almost same in cells treated with 80 and 160 µM IPTG.</p

Primers used in this study.

<p>Primers used in this study.</p

Fold sensitization of <i>yidC</i> antisense expressing <i>E. coli</i> DH5α cells to various antibacterial agents.

<p>Various antibiotics belonging to six different classes based on their mode of action namely cell wall synthesis inhibition (vancomycin and phosphomycin), protein synthesis inhibition (kanamycin and hygromycin), nucleic acid synthesis inhibition (mitomycin and novobiocin), cell membrane disruption (polymyxin B and colistin), metabolic antagonism (trimethoprim) and fatty acid synthesis inhibition (triclosan) as well as some plant natural products such as membrane bound ATPase activity inhibiting essential oils eugenol and carvacrol, cell division protein FtsZ inhibiting alkaloid berberine as well as some other plant natural products named in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0057370#s2" target="_blank">materials and methods</a> (data not shown) were screened for sensitization of YidC depleted strain of <i>E. coli</i> DH5α as compared to control cells (<i>E. coli</i> DH5α harbouring only vector or expressing antisense of <i>murA</i> essential gene). Fold sensitization represents the change in antibiotic IC50 values upon antisense induction. <i>E. coli</i> DH5α cells expressing <i>yidC</i> antisense showed considerable sensitization to eugenol (16 fold) and carvacrol (8 fold) as compared to control cells. However other natural products and antibiotics with known mode of action did not result in fold sensitization equal to or greater than that of <i>murA</i> antisense expressing E. coli cells to phosphomycin (positive control).</p