Observation of B̅0→D(*)0pp̅

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Feedback, computation, and robust learning algorithms

奈良先端科学技術大学院大学博士(工学)doctoral thesi

Analyses of heterogeneous deformation and subsurface fatigue crack generation in alpha titanium alloy at low temperature

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Differential effect of SycO overproduction on cytosolic Yop levels in the presence of calcium ions

<p><b>Copyright information:</b></p><p>Taken from "The type three secretion chaperone SycO is integrated into the Yop regulatory network and binds to the Yop secretion protein YscM1"</p><p>http://www.biomedcentral.com/1471-2180/7/67</p><p>BMC Microbiology 2007;7():67-67.</p><p>Published online 5 Jul 2007</p><p>PMCID:PMC1933539.</p><p></p> Overproduction of SycO was analyzed in parental strain WA-314 and the isogenic deletion mutants Δand Δcultured at 37°C in the presence of 5 mM CaCl. After 2 h 1 mM IPTG was added and cultivation was continued for another 2 h. Pellet fractions were analyzed by immunoblotting with sera raised aginst YopO, SycO and YopH

The SycH overproduction phenotype differs significantly from that of SycO

<p><b>Copyright information:</b></p><p>Taken from "The type three secretion chaperone SycO is integrated into the Yop regulatory network and binds to the Yop secretion protein YscM1"</p><p>http://www.biomedcentral.com/1471-2180/7/67</p><p>BMC Microbiology 2007;7():67-67.</p><p>Published online 5 Jul 2007</p><p>PMCID:PMC1933539.</p><p></p> Analogous to the experimental setup described in legend to Fig. 2, SycH and SycO were overproduced in yersiniae. (A) Coomassie-stained SDS-PAGE after loading of TCA-precipitated fractions of the culture supernatant. (B) Coomassie-stained gel of the corresponding cell lysates

Repression of Yop secretion by SycO overproduction is neither mediated by YscM1 nor by YscM2

<p><b>Copyright information:</b></p><p>Taken from "The type three secretion chaperone SycO is integrated into the Yop regulatory network and binds to the Yop secretion protein YscM1"</p><p>http://www.biomedcentral.com/1471-2180/7/67</p><p>BMC Microbiology 2007;7():67-67.</p><p>Published online 5 Jul 2007</p><p>PMCID:PMC1933539.</p><p></p> Overproduction of SycO was analyzed in parental strain WA-314 and the isogenic deletion mutants Δand Δas described in legend to Fig. 2. Analysis of the culture supernatant is represented in (A) and the cell lysates are shown in (B)

Overproduction of SecB does not cause significant effects on secretion and expression of Yops

<p><b>Copyright information:</b></p><p>Taken from "The type three secretion chaperone SycO is integrated into the Yop regulatory network and binds to the Yop secretion protein YscM1"</p><p>http://www.biomedcentral.com/1471-2180/7/67</p><p>BMC Microbiology 2007;7():67-67.</p><p>Published online 5 Jul 2007</p><p>PMCID:PMC1933539.</p><p></p> Analogous to the experimental setup described in legend to Fig. 2, SecB was overproduced. (A) Coomassie-stained SDS-PAGE after loading of TCA-precipitated fractions of the culture supernatant. (B) Analysis of the corresponding cell lysates; Coomassie-stained gel (upper panel) and corresponding immunoblots to detect SecB, YopH, and YopE (lower panels)

Native gel electrophoresis reveals an interaction between SycO and YscM1

<p><b>Copyright information:</b></p><p>Taken from "The type three secretion chaperone SycO is integrated into the Yop regulatory network and binds to the Yop secretion protein YscM1"</p><p>http://www.biomedcentral.com/1471-2180/7/67</p><p>BMC Microbiology 2007;7():67-67.</p><p>Published online 5 Jul 2007</p><p>PMCID:PMC1933539.</p><p></p> Recombinant proteins as indicated were loaded on Tris-buffered native gels. The gels were Coomassie-stained (A) or alternatively electro-blotted and incubated with antisera raised against SycO (B), and YscM1 (C), respectively. The amount of SycO (20 μg) was approximately equimolar to that of YscM1 (15 μg) and YscM2 (15 μg). SycO/YscM1 complex is indicated by arrowheads. YscM2 does not enter the native gel (pH 8) due to its high isoelectric point (pI 9.79)

SycO overproduction in the mutant strains Δand Δin the presence of calcium ions

<p><b>Copyright information:</b></p><p>Taken from "The type three secretion chaperone SycO is integrated into the Yop regulatory network and binds to the Yop secretion protein YscM1"</p><p>http://www.biomedcentral.com/1471-2180/7/67</p><p>BMC Microbiology 2007;7():67-67.</p><p>Published online 5 Jul 2007</p><p>PMCID:PMC1933539.</p><p></p> Overproduction of SycO was analyzed in parental strain WA-314 and the isogenic deletion mutants Δand Δcultured at 37°C in the presence of 5 mM CaCl. After 2 h 1 mM IPTG was added and cultivation was continued for another 2 h. Pellet fractions were analyzed by immunoblotting with sera raised aginst YopO, SycO and YopH

アルコール混合燃料によるディーゼル機関の燃焼変動に関する研究*

application/pdfThis paper presents the characteristics of cycle-to-cycle combustion variations in a diesel engine. The combustion variations are particularly significant for low cetane number fuels like alcohol, and cause deterioration in thermal efficiency as well as rough combustion. Combustion variations appear in various forms, such as variations in ignition lag, indicated mean effective pressure, maximum combustion pressure, or combustion rate. These variations are largely caused by the variable ignition lag. This paper also reports the characteristics of CPL related to combustion noise in combustion variationsdepartmental bulletin pape