37 research outputs found
教育システムの動態分析のための指標開発とデータベース作成
本研究の目的は、次の二つにある。第一は、諸々の官庁統計のなかから、教育に関連する統計を抽出、収集し、それを時系別分析が可能なように、編集・整理すること、第二には、このようにして収集・編成・整理されたデ-タをもとに、教育システムの動態分析が可能なような諸々の指標を開発する、の二つである。第一の目的を実現するために、我々は学校基本調査、家計調査、賃金構造基本調査、就業構造基本調査の4つの調査を選び、それらに収録されている統計デ-タのうち、重要かつ社会的需要が高いと思われる統計をとり出し、それについて編集、整理を行った。またこれらのデ-タセットが誰れでも容易に利用できるようなマニュアルを作成した。このようにして完成されたデ-タセットは、大小さまざま合計すると51本に達した。ただし、これらはデ-タセットのマニュアルまで完成されたもので、この他にまだマニュアルを作成中のものが、かなりの数ある。一般に上記の官庁統計は年度によって、分類カテゴリ-が変ったり、また編集方式が変更されたりして、時系列的一貫性があるわけではない。上記51本のデ-タセットは、このように年々変わっている統計デ-タを、一つの統一されたフォ-マットに編成することができたもので、それができなかったデ-タ、また統一されたフォ-マットにまとめられたとしても、年度が短いものなどは、まだ多く残っている。このように編集されたデ-タをもとに、我々は各種の分析を実施してきたが、これらの分析結果のうち、比較的に社会的需要の高いものに関しては、これまで逐次刊行してきている。その例をあげるならば、61年を起点とし、70年度までの各都道府県ごとの、小・中学校教員需要数についての推計である。この推計には、小・中学校生徒数、年齢別教員数などのデ-タセットを利用して、推計作業を行った。この他には、家計調査をもとに所得階層別就学率を算出した。科学研究費補助金 研究種目:試験研究(2) 課題番号:59810007 研究代表者:潮木 守一 研究期間:1984-1986年度research repor
X-ray Structural Studies of the Histidine-containing Phosphotransfer Domain of the Anaerobic Sensor Kinase ArcB from Escherichaia coli
奈良先端科学技術大学院大学博士(バイオサイエンス)doctoral thesi
Evaluation of the First order Kinetic Approximation in Heterogeneous Catalysis*
application/pdfThe validity of first order kinetic approximation in heterogeneous catalysis has been evaluated by using the transient response method. As an example of a solid catalysed reaction, the oxidation of carbon monoxide has been studied on four different metal oxides MnO_2, Cr_2O_3, Pb_3O_4, and ZnO. The steady stale rate analysis apparently met first order kinetics with respect to the concentration of CO for all the catalysts. The Arrhenius plots of the apparent first order rate constant obtained clearly gave a good straight line and estimated the apparent activation energy of the reactions on each catalyst. A simple first order kinetic model due to surface reaction controlling is proposed to explain the transient state and the steady stale of reaction. Based on the models proposed, the transient response curves of carbon dioxide caused by the stepwise change in the concentration are simulated by using personal computers The results exhibit a large deviation of the calculated curves from the experimental ones especially at the transient state whereas the steady state values of both are in good agreement. Four different dual path models have separately been proposed for each of the four catalysts. The models consistently explain the characteristic mode of the transient response curves obtained for each catalyst and under wide experimental conditions. The activation energy of the slowest elementary step is evaluated for all the catalysts, and some of the values deviate largely from those estimated from the apparent first order kinetic models.departmental bulletin pape
Comparative relative miRNA expression of miRNAs miR-146b-5p, miR-203, miR-181a* and miR-181a after C/EBPβ down-regulation—deep sequencing and RT-qPCR of ALK+ ALCL cell lines.
<p>Quantification of three by C/EBPβ knockdown significantly regulated miRNAs miR-146b-5p (upper panel), miR-203 (second panel), miR-181a* (third panel) and additionally miR-181a (lower panel), in ALK+ ALCL cell lines. (<b>A</b>) Deep sequencing results of ALK+ ALCL cell lines transduced with pF or pF-C/EBPβ shRNA. Results are depicted as base mean values from triplicates. (<b>B</b>) RT-qPCR analysis of miRNAs 146b-5p, 203, 181a* and 181a in pF and pF-C/EBPβ (shaded) transduced ALK+ ALCL cells four days after infection. Values were normalized to RNU6B and data were analysed according to the 2<sup>-ΔΔCp</sup> method. Results are depicted as miRNA levels relative to untreated SUDHL-1 cells. Error bars indicate standard deviation of triplicates.</p
Principal Component Analysis.
<p>2D scatter plot shows principal component analysis (PCA) of miRNA deep sequencing data. The two axes represent the first two principal components (PCs) from the principal component analysis. The values in brackets indicate the amount of variation in the data that can be explained by the PC. The percent of variation given by a particular PC is indicated in the axis label. Points are colored by sample type. Samples were analyzed in triplicates. The graph shows a clear separation by principal component 1 between normal T cells (grey) and ALCL cells. The principal component 2 separates ALK+ and ALK- ALCL (light blue).</p
Analysis of C/EBPβ regulated miRNAs.
<p>(<b>A</b>) Western Blot analysis of the C/EBPβ isoforms LAP* and LAP in the transduced SR786 cells three days after infection. Each lane of the Western Blot contains 20 μg protein extract. Tubulin was used as loading control. SR786 = uninfected cells, pRRL = empty virus, pRRL-LAP* = virus containing the C/EBPβ isoform LAP* sequence, pRRL-LAP = virus containing the C/EBPβ isoform LAP sequence. (<b>B</b>) RT-qPCR analysis of miRNAs 146b-5p, 203, 181a* and 181a in untreated, mock-treated and pRRL.PPT.SF.i2GFPp (containing LAP and LAP* isoforms) transduced ALK+ ALCL cell line SR-786. Values were normalized to miR-106b and data were analysed according to the 2<sup>-ΔΔCp</sup> method. Results are depicted as miRNA levels relative to untreated SR-786 cells. (<b>C</b>) RT-qPCR analysis of miRNAs 146b-5p, 203, 181a* and 181a in primary ALCL cases (4 ALK+ and 5 ALK- ALCL cases). For RT-qPCR quantification values were normalized to miR-106b and data were analysed according to the 2<sup>-ΔΔCp</sup> method. Results are depicted as miRNA levels relative to mean value of ALK+ ALCL levels. For statistical analysis of RT-qPCR results a Wilcoxon rank-sum test was used (*p<0.05).</p
