平安時代の梵鐘生産

2009-03-31departmental bulletin pape

Observation of the Decay B0→D±D*∓

journal articl

Accuracy in Estimating the Mix Proportions of Hardened Concrete

application/pdfIt is necessary to know and control inner factors which influence durability when structures are designed and constructed. The most important factor is mix the proportions, especially the water-cement ratio. Therefore, the accuracy of estimating the water-cement ratio is the most important point in evaluating durability and estimating life time. However, in the case of existing concrete structures, there is no documentation in most cases. Consequently, in many cases estimation of the water-cement ratio from a hardened concrete sample is necessary for existing structures. This method is shown in papers of CAJ and JSCE. But there are few experiments and little consideration about the accuracy of estimating mix proportions of hardened concrete. In this paper, accuracy in estimating mix proportions of hardened concrete is studied. And the following results were attained. (1) In order to improve the accuracy of estimating mix proportions, the accuracy of quantifying CaO and measuring attached water must be improved. (2)The test of attached water measurement must be conducted before strength tests on the specimen for estimating mix proportions, because large errors in estimating the amounts of attached water will influence the estimation of the water-cement ratiodepartmental bulletin pape

Plenary discussion (International symposium on the teaching of English in Asia: Locating the teaching of English in Japan in Asian contexts: what we can learn from China and Korea)

departmental bulletin pape

サンガク レンケイ ニ トモナウ リエキ ソウハン エノ タイオウ ノ タメノ ガイドライン ノ サクセイ カゾウ ジレイ ニ モトヅク アンケート チョウサ ニヨル ケントウ

協力: 東北大学, 筑波大学, 東京大学, 東京工業大学, 名古屋大学, お茶の水女子大学, 北陸先端科学技術大学院大学, 九州大学, 科学技術政策研究所, (株)富士通総研, (財)理工学振興会[本編]表紙[本編]1-4: 序にかえて－利益相反プロジェクトの目的、経緯および本年度報告書の要約[本編]5-8: 第1章 アンケートの実施方法、および回答者の構成と利益相反への関心[本編]9-49: 第2章 事例に関するアンケートの結果と解析[本編]50-63: 第3章 利益相反ガイドラインの案－特許と兼業について－[本編]64-66: 終章 おわりに[資料編]表紙、目次[資料編]1: 第1部 大学関係者およびTLO関係者へのアンケート 1. プロジェクトからの依頼状[資料編]2: 2. 文部科学省 技術移転推進室長から各大学への依頼状(事務連絡)[資料編]3: 3. 利益相反の事例に関するアンケート調査[資料編]4: 4. アンケート補足説明[資料編]5: 5. 参考資料 5-1) 参考資料集の表紙「参考資料について」[資料編]6: 5-2) 参考資料-1「金銭的利益の公開」(Nature誌記事の抄訳)[資料編]7: 5-3) 参考資料-2「協働と知の創造：産学共同研究イニシアティブ」(Business-Higher Education Forum報告書)の概要[資料編]8: 5-4) 参考資料-3「Business-Higher Education Forum報告書に収載された産学連携の実施と問題点に関する精選された５つの事例」[資料編]9: 6. 回答用紙[資料編]10: 第2部 産業界(関西経済連合会・科学技術委員会委員)へのアンケート 1. プロジェクトからの依頼状(産業界用)[資料編]11: 2. 関西経済連合会科学技術委員会委員各位への依頼状[資料編]12: 3. 利益相反の事例に関するアンケート調査(産業界用)[資料編]13: 6. 回答用紙（産業界用)[資料編]14: 第3部 回答意見集(各事例ごとに大学関係者、TLO関係者、産業界の順にファイル) 1. 事例1: 特許出願と学術発表[資料編]15: 2. 事例2: 余暇発明の届出[資料編]16: 3. 事例3: 科研費周辺の成果の特許の帰属[資料編]17: 4. 事例4: 個人有の発明の出願ルート: TLOの使用と「大学への忠誠」[資料編]18: 5. 事例5: 奨学寄附金と発明譲渡[資料編]19: 6. 事例6: コンサルティング兼業 その1) 収入，および兼業とそれ以外の企業との関係の重複[資料編]20: その2) 兼業と公的便宜の使用[資料編]21: 7. 事例7: 個人的収入と公的決定の関係(いわゆる『お手盛り』)[資料編]23: 9. 産業界による「大学の産学連携への取組み」についての問題点指摘[資料編]22: 8. 利益相反のガイドライン作成に関する自由意見[資料編]24: 補遺 全米大学協会報告書「個人および大学レベルの金銭的利益相反」解説boo

Analysis of the particles.

<p>A,B-Proteins were separated by elecrtrophoresis in Tricine buffer on 16% polyacrylamide gel. Western blotting was performed with polyclonal antibody against histone H3 (Upstate) (A) and against VP1 (B). M–size marker; 1–Nuclear extracts used in the packaging reaction; 2–In vitro assembled particles, fraction 9 of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000765#pone-0000765-g004" target="_blank">Fig. 4</a>. 3–wild type SV40. C-Analysis of the packaged DNA. Particles purified by ultrafiltration were treated with Tris base (200 mM) in presence of 25 mM EGTA and 25 mM DTT for 1 hr at 37°C. DNA was extracted by phenol-chloroform treatment in presence of 1% SDS, and analyzed by Southern blotting with pGL3-control as a probe.</p

Parameters affecting the in vitro packaging reaction.

<p>Effect of temperature (A) and concentration of monovalent salts (B) present during the re-association reaction (step B) on the yield, measured as titer of luc transducing units.</p

Equilibrium sedimentation in CsCl gradient.

<p>The reaction products, 2 ml, were fractionated on a CsCl density gradient in stabilization buffer at pH 5.2 (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000765#s4" target="_blank">Materials and Methods</a>). VP1 was analyzed by Coomassie and Bradford, DNA by real-time quantitative PCR and <i>luc</i> TU as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000765#s4" target="_blank">Materials and Methods</a>.</p

Structure of the nanoparticles.

<p>Transmission electron microscopy pictures of (A) VLPs, (B) <i>in vitro</i> packaged nanoparticles, and (C) wild type SV40. Samples were adsorbed onto formvar-carbon-coated copper grids and stained with 1% sodium phosphotungstate, pH 7.0. The samples were viewed in a Philips CM-12 electron microscope, using a voltage of 100 kV, and photographed at a magnification of 53,000×.</p

The reaction requires cellular factors.

<p>A–VLPs were purified on sucrose gradient from nuclear extracts harvested on day 3. M-molecular weight marker; VLPs–purified VLPs used in the packaging experiment (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0000765#pone-0000765-t001" target="_blank">Table 1</a>). B-Nuclear extracts were harvested from infected Sf9 cells at different time points after infection, as designated on top, and analyzed by SDS-PAGE and Coomassie-blue staining. M-molecular weight marker; Mock-nuclear extract of Sf9 cells infected with wild type baculovirus; SV40-nuclear extract of CV1 cells infected with wild type SV40; BSA-1 µg BSA, a standard for band intensity. C-Activity of the nuclear extracts shown in part B. ○-Packaging activity measured as titer (left ordinate); □-VP1 level estimated from A (right ordinate).</p