24 research outputs found
A climatological study on surface airflow patterns in Central Japan
Get PDF筑波大学University of Tsukuba博士（理学）Doctor of Philosophy in Scienceこの研究は，関東・中部両地方を含む中部日本におけるメソ・スケールの地上気流の分布型について，年間を通しての出現の実態を明らかにするとともに，その出現に関係する気象要因を解明したものである。 論文の内容は，（1）クラスター分析による気流パターンの分類と，気流パターンの出現の時間的。季節的特徴および気圧配置型との関係，（2）各気流パターンと総観規模の気圧傾度および熱的効果（昼夜における加熱・冷却）との関係，（3）気流パターンのこれら二つの要因（総観規模の気圧傾度と熱的効果）に対する依存度の解明の三つから成立っている。 （1）は中部日本における地上気流パターンの気侯的実態を解明した部分である。AMeDAS観測網の資料により得られた1464枚の地上風分布図をクラスター分析により，15種類の型に分類し，各型の出現に，昼夜や季節の特徴があること，特定の気圧配置と対応関係があることなどが明らかになった。 （2）は各気流型の出現する気象条件として支配的と考えられる，総観規模の気圧傾度と，気層の加熱冷却を表わす熱的効果との関係を定量的に調べた。その結果，各気流型の出現に気圧傾度の差違が極めて敏感に作用すること，熱的効果は一般風が弱い場合だけでなく，一般風が強い場合でも，冷却から加熱に状態が移行するにつれて大気安定度を媒介して，影響を及ぼしていることが分った。 （3）では，上記の二つの要因を座標軸とするグラフ上に各気流型をプロットしたところ，それらは，グラフ上の別々の領域に位置づけられ，気流型間で重なり合う部分はわずかであることが示された。このことから，気流型の特徴は，これら二つの要因にほとんど依存しているといえる。 また海陸風や山谷風や夜間接地逆転層など熱的原因が支配的な風系の発現の有無に対応する臨界的気圧傾度として1.2mb/100mが得られた。この結果は従来，気象業務の現場で経験的に知られていた，総観規模の傾度風速10m/sとほぼ一致する。 Distributions of climatic elements are closely related to surface airflows, which have an ability to transport climatic properties from one place to another. Therefore, it is important to survey surface airflow patterns in large-scale (several hundreds kilometers). ...1988doctoral thesi
Experimental Study on Flows and Bed Change Characteristics in Curved Open Channels
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Representative data for subcellular fractionation and immunoblot analysis of Survivin in clinical samples.
No full text<p>The oultline of the method was illustrated in Figure S6. Normal and Cancerous tissues from human colorectal cancer patients without metastases (<i>Case A</i>) or with metastases (<i>Case B</i>) were lysed. The cell lysate was fractionated into the detergent-soluble cytoplasmic (<i>C</i>), and nuclear (<i>N</i>) fractions and the detergent-insoluble pellet (<i>P</i>) fraction for immunoblot analysis with anti-Survivin, anti-α-tubulin, anti-H3-histone, and anti-β-actin.</p
Overexpression of Survivin does not significantly protect radiations-induced apoptosis, but does protect apoptosis under other stresses.
No full text<p><b>A.</b> Frequency of apoptosis in CHE cells (with p53+/+ and p53−/−) transfected with pEGFP-empty and pEGFP-Survivin after treatment with X-irradiation (10 Gy) and UV-C (10 J/m<sup>2</sup>). The transfected cells were exposed to IR or UV-C at 24 h after transfection. Transfection frequencies were 80–90%, and EGFP-positive cells were counted for Annexin V-positive or -negative cells (<i>upper panel</i>) and TUNEL assay-positive or -negative cells (<i>lower panel</i>). X-irradiated CHE-p53−/− cells had significantly greater fraction of apoptosis-positive cells when compared to X-irradiated CHE-p53+/+ cells (<i>P</i><0.03 for Annexin V staining and <i>P</i><0.08 for TUNEL assay), and UV-C irradiated CHE-p53−/− cells had significantly greater fraction of apoptosis-positive cells when compared to UV-C irradiated CHE-p53+/+ cells (<i>P</i><0.001 for Annexin V staining and <i>P</i><0.02 for TUNEL assay). Apoptosis-positive cells were not significantly decreased in pEGFP-Survivin-transfected cells when compared to pEGFP-transfected cells in each case (<i>P</i>>0.4 for Annexin V staining and <i>P</i>>0.4 for TUNEL assay). Values indicate means ± S.D. (n = 3). <b>B.</b> Increase of the retention in the lung after intravenous injection of EGFP- or EGFP-Survivin-expressing cells. The number of surviving CHE-p53−/− cells in the lung was significantly increased when compared to the number of surviving control cells expressing EGFP. Values indicate means ± S.D. of six mice. *Significant difference (<i>P</i><0.005). <b>C.</b> Caspase-3 activation in CHE-p53−/− cells transfected with pEGFP-empty and pEGFP-Survivin after anoikis induction. The transfected cells were detached from extracellular matrix and simultaneously serum-starved to induce anoikis at 24 h after transfection. Cells were suspended in serum-free medium for 6–36 h, harvested, and lysed in Laemmli SDS-sample buffer for immunoblot analysis with anti-activated caspase-3 antibody. Transfection frequencies were checked by using fluorescence microscopy and confirmed to be 80–90%. <b>D.</b> Caspase-3 activation in CHE-p53−/− cells transfected with pEGFP-empty and pEGFP-Survivin after treatment with UV-C (10 J/m<sup>2</sup>). The transfected cells were exposed to UV-C at 24 h after transfection. Cells were cultured for 24 h, harvested, and lysed in Laemmli SDS-sample buffer for immunoblot analysis with anti-activated caspase-3 antibody. Transfection frequencies were checked by using fluorescence microscopy and confirmed to be 80–90%.</p
EGFP-Survivin and XIPA found in the detergent-soluble fractions.
No full text<p><b>A.</b> Subcellular fractionation and immunoblot analysis of activated caspase-3, Survivin, and XIAP. Transfection frequencies were checked by using fluorescence microscopy and confirmed to be 80–90%. Cells were kept in serum-free medium for 3–6 h, harvested, and lysed. The cell lysate was fractionated into the detergent-soluble cytoplasmic (<i>C</i>), and nuclear (<i>N</i>) fractions and the detergent-insoluble pellet (<i>P</i>) fraction for immunoblot analysis with anti-activated caspase-3, anti-Survivin, anti-GFP, anti-XIAP, anti-α-tubulin, anti-H3-histone, and anti-β-actin. <b>B.</b> Protein-protein interactions between EGFP-Survivn and XIAP. Cell lysate from the detergent-soluble cytoplasmic fraction was immunoprecipitated by anti-XIAP antibody and anti-GFP antibody and subsequently immunoblotted with anti-GFP antibody (<i>lower panel</i>) and anti-XIAP antibody (<i>upper panel</i>), respectively. EGFP-Survivin and XIAP were co-imunoprecipitated only in cell lysate form EGFP-Survivin-expressing cells (<i>lane 2</i>).</p
Clinicopathological significance of the detergent-soluble cytoplasmic Survivin expression in colorectal cancer.
No full texta<p>Tumor invasion of mucosa (m), submucosa (sm), muscularis propria (mp), subserosa (ss), penetration of serosa (se), and invasion of adjacent strucures (si).</p>*<p>Significant difference.</p
