Aerotolerance and multilocus sequence typing among Campylobacter jejuni strains isolated from humans, broiler chickens, and cattle in Miyazaki Prefecture, Japan

Campylobacter jejuni is one of the leading causes of human gastroenteritis in Japan. As chickens and cattle are common reservoirs for C. jejuni, this microaerophilic, stress-sensitive bacterium can overcome and survive various stress conditions during zoonotic transmission, particularly foodborne, to humans. How C. jejuni overcomes stress conditions is, however, unclear. In the present study, 70 C. jejuni strains isolated from various sources (26 human, 20 broilers, and 24 cattle isolates) in Miyazaki, Japan, from 2010 to 2012, were subjected to multilocus sequence typing (MLST) and aerotolerance testing (aerobic shaking at 200 rpm). The results demonstrated that C. jejuni strains from Miyazaki belonged to 12 clonal complexes (CCs) and 43 sequence types (STs). CC-21 and CC-460 were mainly detected in human clinical strains. Most tested strains were aerotolerant, and only one (1.4%) was deemed sensitive to aerobic stress. Approximately 40% strains survived the 24-hr vigorous aerobic shaking at 200 rpm, and these hyper-aerotolerant strains were more prevalent in broiler and cattle isolates than in human isolates. Phylogenetic analysis divided the strains into five clusters, each showing a different pattern of host association. Thus, we have demonstrated for the first time that C. jejuni strains with increased tolerance to aerobic stress are highly prevalent in broilers and cattle in Miyazaki, Japan, and that certain clonal populations are frequently implicated in human infection in this area.Citation: Savek KIATSOMPHOB, Takako TANIGUCHI, Elpita TARIGAN, Khin Maung LATT, Byeonghwa JEON, Naoaki MISAWA, Aerotolerance and multilocus sequence typing among Campylobacter jejuni strains isolated from humans, broiler chickens, and cattle in Miyazaki Prefecture, Japan, Journal of Veterinary Medical Science, 2019, 81 巻, 8 号, p. 1144-1151, 公開日 2019/08/24, Online ISSN 1347-7439, Print ISSN 0916-7250, https://doi.org/10.1292/jvms.19-022

Precise Measurement of B Meson Lifetimes with Hadronic Decay Final States

journal articl

研究速報 : 反応速度論に基づくアルカリ･シリカ反応性の定量的な判定試験方法の開発 : 溶出試験のモデル化

departmental bulletin pape

Plots of squared correlations of allele frequencies (r) against distance between pairs of polymorphic sites in three genes: a) EST28, b) EST13, and c) EST1

<p><b>Copyright information:</b></p><p>Taken from "Nucleotide diversity and linkage disequilibrium in 11 expressed resistance candidate genes in "</p><p>http://www.biomedcentral.com/1471-2229/7/43</p><p>BMC Plant Biology 2007;7():43-43.</p><p>Published online 4 Aug 2007</p><p>PMCID:PMC1978496.</p><p></p> Curves show nonlinear regression of ron weighted distance

Study on Damage Evaluation of Joint in Steel Member by using Local Vibration Excitation

This paper deals with the local excitation method of vibration test by using mutilayer piezoelectric actuators as input device to specimen for the purpose of detecting comparatively small damage of steel structural member, and measures change of an vibration characteristics. It is shown that the vibration test by using mutilayer piezoelectric actuator is effective to damage detection of steel structural member. After releasing bolt supports of a steel structural member, the vibration test is carried out, and the validity of damage detection is examined. The analytical software system using genetic algorithm (GA) to evaluate the location and level of released bolts as a model of damage, is examined. And effectiveness of that system is verified by comparing with the experimental results.application/pdfjournal articl

NAIST エイゴ コウトウ ハッピョウ ワークショップ

video/mp4講演者所属: 日本テキサス・インスツルメンツ代表取締役社長講演者所属: 奈良先端科学技術大学院大学　Professor in Scientific Writing in English講演者所属: 奈良先端科学技術大学院大学バイオサイエンス研究科助教授講演者所属: 奈良先端科学技術大学院大学バイオサイエンス研究科助手講演者所属: 奈良先端科学技術大学院大学情報科学研究科博士課程後期vide