犬の低蛋白食給与時における体蛋白異化の指標としての尿中クレアチニン/尿素窒素比の有用性の検討

宮崎大学博士（獣医学）2019年度doctoral thesi

Impact of Vitamin E‐Coated Membrane Hemodiafilter on Serum Albumin Redox State in the Acute Kidney Injury Pig Hemodialysis Model

Background Several studies have evaluated the biocompatibility of dialysis membranes. The use of vitamin E-coated membranes has been reported multilaterally in in vitro and clinical studies. Nevertheless, the effect of vitamin E-coated membranes on the redox state of serum albumin, which forms the largest fraction of reactive sulfhydryl groups, has not been reported. Methods Hemodiafiltration (HDF) with and without a vitamin E-coated hemodiafilter (V-RATM group and ABHTM groups, respectively) was performed in an acute kidney injury pig model to determine whether changes in the serum albumin, the oxidized albumin (OxiALB), and the reduced albumin (RedALB) levels differ between the two groups. Results Analyses were conducted 22–24 times in the V-RATM group and 16–18 times in the ABHTM group, excluding missing data. The serum albumin levels decreased in both groups after nephrectomy; however, the decrease observed in the V-RATM group was significantly lesser than that in the ABHTM group. RedALB levels were significantly higher in the V-RATM group; in contrast, OxiALB levels did not differ between the two groups. A significant positive correlation was observed between the serum albumin and RedALB levels. Conclusions The present study demonstrated that HDF performed using a vitamin E-coated hemodiafilter effectively minimized the reduction in serum albumin and RedALB levels compared to the vitamin E-non-coated hemodiafilter in an acute kidney injury pig model.Citation: Shouichi Fujimoto, Masahide Koremoto, Shushi Yamamoto, Hiroshi Umeno, Yusuke Sano, Toshihiro Tsuruda, Impact of Vitamin E‐Coated Membrane Hemodiafilter on Serum Albumin Redox State in the Acute Kidney Injury Pig Hemodialysis Model, Artificial Organs, 2025-03-19, https://doi.org/10.1111/aor.1498

Retrospective analysis of canine gallbladder contents in biliary sludge and gallbladder mucoceles

The pathophysiology of canine gallbladder diseases, including biliary sludge, gallbladder mucoceles and gallstones, is poorly understood. This study aimed to evaluate the component of gallbladder contents and bacterial infection of the gallbladder in order to elucidate the pathophysiology of biliary sludge and gallbladder mucoceles. A total of 43 samples of canine gallbladder contents (biliary sludge, 21 and gallbladder mucoceles, 22) were subjected to component analysis by infrared spectroscopy, and the resultant infrared spectra were compared with that of swine mucin. Of the 43 samples, 41 were also evaluated by aerobic and anaerobic bacterial culture. The contents of 20 (95.2%) biliary sludge and 22 (100%) gallbladder mucocele samples exhibited similar infrared spectra as swine mucin. Although biliary sludge and gallbladder mucocele contents exhibited similar infrared spectra, one sample of biliary sludge (4.8%) was determined to be composed of proteins. The rate of bacterial infection of the gallbladder was 10.0% for biliary sludge and 14.3% for gallbladder mucoceles. Almost all of the identified bacterial species were intestinal flora. These results indicate that the principal components of gallbladder contents in both gallbladder mucoceles and biliary sludge are mucins and that both pathophysiologies exhibit low rates of bacterial infection of the gallbladder. Therefore, it is possible that gallbladder mucoceles and biliary sludge have the same pathophysiology, and, rather than being independent diseases, they could possibly represent a continuous disease. Thus, biliary sludge could be considered as the stage preceding the appearance of gallbladder mucoceles.Citation: Mizutani S, Torisu S, Kaneko Y, Yamamoto S, Fujimoto S, Ong BH, Naganobu K. Retrospective analysis of canine gallbladder contents in biliary sludge and gallbladder mucoceles. J Vet Med Sci. 2017 Feb 28;79(2):366-374. doi: 10.1292/jvms.16-0562. Epub 2016 Dec 17. PMID: 27990011; PMCID: PMC5326943

Usefulness of Urinary Creatinine/Urea Nitrogen Ratio as Indicator of Body Protein Catabolism in Dogs Fed Low Protein Diets

Citation: Yamamoto S, Ohta Y, Hasegawa E, Hashida S, Kaneko Y, Mizutani S, Ong BHE, Naganobu K and Torisu S (2019) Usefulness of Urinary Creatinine/Urea Nitrogen Ratio as Indicator of Body Protein Catabolism in Dogs Fed Low Protein Diets. Front. Vet. Sci. 6:449. doi: 10.3389/fvets.2019.0044

Effects of a single-bolus bupivacaine injection into the coccygeal spinal canal of rabbits

It has been reported that drugs intended for epidural administration through the lumbosacral junction are accidentally administered into the subarachnoid space frequently in rabbits. Therefore, we evaluated the epidural single-bolus injection technique for the administration of bupivacaine into the coccygeal spinal canal of rabbits. After epidural distribution was confirmed by the injection of iohexol into the coccygeal spinal canal, 0.3 ml/kg 0.5% bupivacaine or 0.3 ml/kg normal saline was injected via the same needle. After the first attempt of iohexol injection, although the contrast was found in the epidural space in all rabbits, the additional contrast was also found in blood vessel in 3 rabbits and in muscular layer in 1 rabbit. Subarachnoid distribution was not observed in any of the rabbits. The time taken to regain normal anal reflex, movement of the hind limbs during walking, conscious proprioception of the hind limbs, and pain sensation of the tail and left hind limb, following coccygeal spinal canal injection, were significantly longer in the bupivacaine group than in the normal saline group. These findings indicated that coccygeal epidural injection of bupivacaine in rabbits may provide anesthesia for the hind limbs, perineum, and tail, but inadvertent vascular entry of the epidural drug may occur.Benedict H.E. ONG, Yuki HIDAKA, Yasuyuki KANEKO, Shushi YAMAMOTO, Shinya MIZUTANI, Satoshi SEKIGUCHI, Shidow TORISU, Kiyokazu NAGANOBU, Effects of a single-bolus bupivacaine injection into the coccygeal spinal canal of rabbits, Journal of Veterinary Medical Science, 2020, 82 巻, 2 号, p. 197-203, 公開日 2020/02/18, Online ISSN 1347-7439, Print ISSN 0916-7250, https://doi.org/10.1292/jvms.19-055

A Computer Software Program for Specifying Water Sampling Sites using the World Wide Web

departmental bulletin pape

Precise Measurement of B Meson Lifetimes with Hadronic Decay Final States

journal articl

IT カクメイ オ ササエル フォトニクス

video/mp4講演者所属: 奈良先端科学技術大学院大学物質創成科学教授vide

Outline of Japan-Russia joint Glaciological Research on Sofiyskiy Glacier, Russian Altai Mountains in 2000 and 2001

This paper focuses on field activity by the “Japan-Russia Joint Glaciological Research on Sofiyskiy Glacier, Russian Altai Mountains” carried out during 15-24 July 2000 and 6-17 July 2001. The purpose of this investigation was to reconstruct climate and environment records in the past few decades through ice core study. Three ice cores 25.1m, 12.3m and 9m deep were recovered and two pits of 3 and 4.5m deep were made on the accumulation area of Sofiyskiy Glacier (49°47′10″N, 87°43′48″E; 3435m a.s.l.). The deepest 25.1m core will preserve the environmental record during the last 10 to 20 years. Ice core samples were cut and melted at the research site, and transported to Japan for more detailed analyses, such as oxygen isotopes, microparticles, pH, anions, cations and bacteria content. Cores consisted of firn and ice layers. It was found that the borehole temperature was 0℃ from the surface to 8m depth and also from 16m to 25m depth. The temperature between 8 and 16m was negative with the minimum at 10m depth (-0.1℃ for the 2000 borehole and -0.3℃ for the 2001 borehole). Meteorological observations were also carried out. After the investigation on Sofiyskiy Glacier in 2001, reconnaissance survey of glaciers near the Russia-China-Mongolia border region was carried out and surface snow was sampled at two sites.application/pdfjournal articl

Pancreatic enzyme activity in the bile of healthy cats and its association with biliary morphology

Background In human medicine, congenital maljunction of the common bile duct (CBD) and main pancreatic duct (MPD), or pancreatobiliary maljunction (PBM), is a known cause of cholecystitis. Objective Pancreatic enzyme activity in the bile (a diagnostic marker for PBM) of healthy cats was measured to determine normal values and evaluate its relationship with biliary morphology. Animals Fifty-two healthy cats. Methods Cross-sectional study of the biliary tracts of healthy cats during laparoscopic ovariohysterectomy and measurement of pancreatic enzyme activity in bile. The cats were divided into groups A and B based on the ratio of the diameter of the cystic duct (CD) to the CBD. The normal ratio was 3.4. Pancreatic enzyme activity in bile was compared between the groups. Results The CBDs were straight in all cases, whereas the CDs were variably tortuous or dilated. Amylase activity in the bile (median, 3.4, had significantly higher amylase activity (median, 109 U/L; range, <100-591 U/L) in the bile than did group A (median, <100 U/L; range, <100-238 U/L), which had a CD/CBD ratio <3.4 (P = .0009). Conclusions and Clinical Importance The results suggest that a dilated CD is associated with reflux of pancreatic juice. In the future, it will be necessary to examine the clinical usefulness of these findings by measuring pancreatic enzyme activity in the bile of cats with cholangitis.Cite: Fujimoto S, Torisu S, Kaneko Y, Mizutani S, Yamamoto S, Naganobu K, Nakamura K. Pancreatic enzyme activity in the bile of healthy cats and its association with biliary morphology. J Vet Intern Med. 2021 Jul;35(4):1780-1788. doi: 10.1111/jvim.16196. Epub 2021 Jun 12. PMID: 34117814; PMCID: PMC8295664