国際研究グループによって提案された肝切除後の肝不全の予測：99mTc-ガラクトシルヒト血清アルブミンシンチグラフィによる残存肝機能予測

宮崎大学博士（医学）2020年度doctoral thesi

Prediction of Posthepatectomy Liver Failure Proposed by the International Study Group of Liver Surgery : Residual Liver Function Estimation With 99mTc-Galactosyl Human Serum Albumin Scintigraphy

PURPOSE: The International Study Group of Liver Surgery (ISGLS) proposed a definition and grading system for posthepatectomy liver failure (PHLF). We evaluated the usefulness of residual liver function estimation using Tc-99m-galactosyl human serum albumin (99mTc-GSA) for the prediction of PHLF. METHODS: Patients with liver tumors (n = 136) and scheduled for hepatectomy underwent 99mTc-GSA scintigraphy. Based on their imaging data, the maximal GSA removal rate (GSA R-max）was calculated by multicompartment analysis. We also calculated GSA R-max in the predicted residual liver (GSA-RL) whose volume was determined on computed tomography (CT) scans. We compared the age, gender, 15-min indocyanine green retention rate, albumin-, bilirubin-, hyaluronic acid-, and type 4 collagen level, the Child-Pugh classification, residual liver volume, residual liver percentage, GSA R-max, and GSA-RL in patients with/without PHLF. Uni- and multivariate logistic analyses were used for statistical assessments. RESULTS: Of 136 patients, 17 (12.5%) met the ISGLS criteria for PHLF (ISGLS-PHLF). There was a statistically significant difference in the age, albumin level, Child-Pugh classification, residual liver volume, residual liver percentage, GSA R-max, and GSA-RL between patients with/without PHLF. Based on multivariate analysis, GSA-RL and the residual liver volume were significant independent predictors of ISGLS-PHLF (p = 0.004 and p = 0.038, respectively). The odds ratio was 149423 for GSA-RL and 1.003 for the residual liver volume. CONCLUSION: GSA-RL calculated by 99mTc -GSA scintigraphy was the most useful independent predictor for ISGLS-PHLF.Citation: Mizutani, Y., Hirai, T., Nagamachi, S., Nanashima, A., Yano, K., Kondo, K., Hiyoshi, M., Imamura, N., & Terada, T. (2018). Prediction of Posthepatectomy Liver Failure Proposed by the International Study Group of Liver Surgery: Residual Liver Function Estimation With 99mTc-Galactosyl Human Serum Albumin Scintigraphy. Clinical nuclear medicine, 43(2), 77–81. https://doi.org/10.1097/RLU.000000000000191

An Educational Computer Software Program using Java for Molecular Symmetry and Normal Vibrations

departmental bulletin pape

Precise Measurement of B Meson Lifetimes with Hadronic Decay Final States

journal articl

研究速報 : Anisotropic Deformation and Strength Properties of Wet-Tamped Sand in Plane Strain Compression at Low Pressures (Part 5) : Modelling of Stress-Strain Relation

departmental bulletin pape

Basic Analysis of Measurement Data from Japan in EVEN Project

The second Permanent International Association of Road Congresses?World Road Association International Experiment to Harmonize Longitudinal and Transverse Profile Measure and Reporting Procedures (EVEN Project) was conducted in 1998 in three regions of the world. The aim of the research is to harmonize and correlate measures of road profiles for applications in pavement construction and management. The basic analysis and measurement results for roughness conducted in Hokkaido, Japan, are summarized. The longitudinal profile measurements obtained with seven high-speed profilometers in Japan, their influence on roughness indices such as the international roughness index, and the correlations between roughness indices, together with Japan’s current measurement and evaluation system for roughness, are also compared and analyzed.application/pdfjournal articl

MOESM1 of Kinetics of MSC-based enzyme therapy for immunoregulation

Additional file 1: Figure S1. MSCs convert ATP to Adenosine which can be inhibited by POM-1. (A) For MSCs dosed with 1 mM ATP (500 nmol), adenosine production increases with time. Hydrolyzed ATP and adenosine production were measured at each timepoint to show that 95% of ATP hydrolyzed by cells is converted to adenosine. The maximum amount of ATP hydrolysis predicted by models at this density is 120 nmol (when operating at Vmax) and experimentally we find 118 nmol is hydrolyzed and 112 nmol is converted to adenosine. (B) To inhibit CD39 activity, MSCs were treated for 20 min with 100 µM of POM-1 inhibitor and no measurable adenosine was recorded until 2 h after ATP treatment and remains significantly less than untreated groups. Figure S2. Fibroblast cells hydrolysis of ATP. (A) Expression of ectoenzymes CD73 and CD39 is similar to that of MSCs with normal dermal fibroblasts and pancreatic cancer-associated fibroblasts. CD73 is high expressed, while CD39 is weakly expressed. (B) When exposed to 64 nmol of ATP, both fibroblasts cell types can hydrolyze the ATP completely within 2 h

境界地域史研究から考える引揚げ文学論の意義

departmental bulletin pape

Additional file 1: Figure S1. of Multiple genetically engineered humanized microenvironments in a single mouse

Selection and conformation of lentivial transfected mouse stromal cells. (A) Flow cytometric analysis of GFP mBMSC, (B) Culture-expanded genetically engineered mBMSCs. (Scale bar, 200μm). Figure S2. Characterized secretion of human cytokines from genetically engineered stromal cells in 1 and 3 weeks in vitro culture. Figure S3. hSDF1a ELISA in mouse blood serum. Control mice without scaffold implantation showed a background level of SDF1a signal due to cross-reactivity. This level was used as a baseline and was also observed in growth arrested, which was concluded, as undetectable. The other groups showed measurable levels above background and were concluded to be true hSDF-1a detection. Figure S4. SEM images of growth-competent genetically engineered stromal cell-seeded scaffolds. (A) Cross-sectional images of human soluble factor secreting engineered stromal cell-seeded scaffolds after 6 weeks subcutaneous implantation. Except hTNFa, entire pores were completely filled with tissue cells with no hematopoietic components. (B) Closed-up image of growing engineered stromal cell-seeded scaffolds. Figure S5. Examples of semi-quantitative image analysis using ImageJ. (A) Collagen fiber area estimation from a Masson’s Trichrome staining image, (B) Vasculature area estimation from an immunohistostaining mCD31 and DAPI image. Figure S6. Long-term maintenance of inflammation-mimicking tissue microenvironment indirectly indicates survival and function of growth-arrested hTNFa secreting engineered stromal cells in the implanted scaffolds. (DOCX 2962 kb

Additional file 3: of Quantitative assessment of LASSO probe assembly and long-read multiplexed cloning

Table S2. NGS Read Accounting of Sequenced LASSO E. coli ORF-eome Captures. The table shows raw sequencing output (HiSeq 1 × 50 bp platform) of PCR amplified multiplexed ORF-eome capture. Reads were first aligned to both E. coli genome and LASSO probe backbone sequence, then read mapped exclusively to genomic DNA was cleaned, filtered, and separated according to targets or non-targets (untargeted ORFs and intergenic regions). Also included are the median read depths of gDNA sequencing enrichment. (XLSX 9 kb