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Article信州大学農学部演習林報告 27: 69-81(1990)departmental bulletin pape

Observation of the Color-Suppressed Decay B̅ 0→D0π0

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Sodium Ascorbate induces apoptosis in neuroblastoma cell lines by interfering with iron uptake-2

<p><b>Copyright information:</b></p><p>Taken from "Sodium Ascorbate induces apoptosis in neuroblastoma cell lines by interfering with iron uptake"</p><p>http://www.molecular-cancer.com/content/6/1/55</p><p>Molecular Cancer 2007;6():55-55.</p><p>Published online 30 Aug 2007</p><p>PMCID:PMC2000471.</p><p></p> for 24 hours and iron levels were measured in cell lysates. After terminating the incubation, cells were collected by scraping and washed three times with PBS and then lysed in specific buffer. Iron levels were analyzed with a Cobas Integra 800 system as described under "Material and Method". Values indicated are the mean ± S.D. of three separate experiments

Membrane expression of TfR (CD71) by neuroblastoma cell lines and its modulation by sodium ascorbate

<p><b>Copyright information:</b></p><p>Taken from "Sodium Ascorbate induces apoptosis in neuroblastoma cell lines by interfering with iron uptake"</p><p>http://www.molecular-cancer.com/content/6/1/55</p><p>Molecular Cancer 2007;6():55-55.</p><p>Published online 30 Aug 2007</p><p>PMCID:PMC2000471.</p><p></p> Cells were seeded in six well plates and treated for 6 and 24 hours with increasing concentrations of sodium ascorbate. For each bar group, the concentration was: 0 (control), 0.5 mM, 1 mM, 2 mM and 3 mM. Cells were than washed and incubated with a FITC-coniugated mouse monoclonal antibody specific to human CD71 and analyzed by flow cytometry. The data are the mean ± S.D. from three independent experiments, each in triplicate

Representative example of cytofluorimetric analysis showing the effect of vitamin C alone or in combination with FAC, on the mitochondrial membrane potential of SH-SY5Y (panel A) and HTLA-230 (panel B) stained with JC-1 probe

<p><b>Copyright information:</b></p><p>Taken from "Sodium Ascorbate induces apoptosis in neuroblastoma cell lines by interfering with iron uptake"</p><p>Molecular Cancer 2007;6():55-55.</p><p>Published online 30 Aug 2007</p><p>PMCID:PMC2000471.</p><p></p> In abscissa FL-1 (green fluorescence); in ordinate FL-2 (red fluorescence). Numbers represent the percentage of non-apoptotic red fluorescent cells (R1, R3) and that of apoptotic green fluorescent cells (R2, R4). Sodium ascorbate induces a marked increase in apoptosis which is fully prevented by treatment with FAC. SH-SY5Y and HTLA-230 were incubate in complete medium in the absence (A1, B1) or in presence of 1 mM sodium ascorbate (A2, B2) and 2 mM sodium ascorbate (A4, B4) for 16 hours. Cells were pretreated (A3, A5, B3, B5) with 70 μg/ml of FAC for 3 hours. Comparable results were obtained in three independent experiments

Sodium Ascorbate induces apoptosis in neuroblastoma cell lines by interfering with iron uptake-4

<p><b>Copyright information:</b></p><p>Taken from "Sodium Ascorbate induces apoptosis in neuroblastoma cell lines by interfering with iron uptake"</p><p>http://www.molecular-cancer.com/content/6/1/55</p><p>Molecular Cancer 2007;6():55-55.</p><p>Published online 30 Aug 2007</p><p>PMCID:PMC2000471.</p><p></p>of sodium ascorbate (in the presence or absence of FAC 70 μg/ml). After 16 hours, cells detached in the medium were collected by centrifugation, resuspended and incubated with AnnexinV-FITC. Apoptosis was quantified as increased green fluorescence by flow cytometry. The data are the mean ± S.D. from four independent experiments. Statistical analysis was done by Student's test (*, p < 0.005)

<論文>ミドルリーダーを対象としたサーベイフィードバック型研修の開発と評価―カードを用いたケースメソッド―

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