多元社会・台湾における放送と市民

departmental bulletin pape

Non-Cytotoxic Photodynamic Therapy with Talaporfin Sodium Reduces the Expression of CXCR4 and Enhances Chemotherapeutic Efficacy in Undifferentiated Gastric Cancer Cell Line HGC27

Gastric cancer (GC), particularly the undifferentiated type, is frequently associated with peritoneal metastasis, which significantly worsens prognosis due to its resistance to conventional treatments. Photodynamic therapy (PDT) is localized treatment using a photosensitizer (PS) activated by light of a specific wavelength to generate cytotoxic reactive oxygen species that induce cell death. Severe adverse events were reported from clinical trials investigating PDT for peritoneal dissemination conducted until the early 2000s, leaving its safety and clinical effectiveness unestablished. The present study explored whether “non-cytotoxic” PDT using talaporfin sodium (TS) could enhance efficacy of chemotherapeutic agents in undifferentiated GC cell line HGC27. Cell viability was evaluated with MTT assay following TS-PDT, and the synergistic effect between non-cytotoxic TS-PDT and anticancer drug SN-38 was assessed. Changes in expression of drug resistance markers were analyzed through qRT-PCR, Western blotting, and immunocytochemistry. We found that non-cytotoxic TS-PDT enhanced the efficacy of chemotherapy in the undifferentiated GC cell line and reduced the expression of C-X-C chemokine receptor type 4, a key marker associated with GC stem-like properties. These findings highlight the potential of non-cytotoxic TS-PDT as a synergistic treatment approach. We conclude that non-cytotoxic TS-PDT could enhance drug sensitivity and offers a promising therapeutic strategy for GC

SETDB1-Mediated Chromatin Regulation in Intestinal Epithelial Cells During Intestinal Ischemia-Reperfusion Injury

SET domain bifurcated 1 (SETDB1), a histone H3K9-specific methyltransferase, is crucial for heterochromatin formation and intestinal homeostasis, but its role in intestinal ischemia-reperfusion injury (IRI) remains unclear. This study investigated changes in SETDB1-mediated nuclear chromatin regulation in intestinal epithelial cells (IECs) using an IRI mouse model. Jejunal samples were collected after 75 min of ischemia followed by 24 hr of reperfusion. Sinefungin was administered as a histone methyltransferase inhibitor. Morphologic changes were evaluated using hematoxylin-eosin staining and electron microscopy, and cell-adhesion molecule expression, including ZO-1, E-cadherin, integrin-β4, and laminin, was evaluated using immunohistochemistry. Super-resolution microscopy analyzed intranuclear SETDB1 localization and heterochromatin formation in IECs. IRI-affected jejunum exhibited massive IEC detachment, dilated intercellular spaces, basement membrane damage, and decreased expression of E-cadherin and integrin-β4. Sinefungin prevented these changes, however. The proportion of IECs expressing nuclear SETDB1 throughout the euchromatin was significantly higher in IRI-affected jejunum (77.8%) than sham-treated (3.0%) or sinefungin-treated, IRI-affected jejunum (2.7%). The proportion of IECs with decreased heterochromatin was significantly higher in sinefungin-treated, IRI-affected jejunum (84.3%) than untreated IRI-affected jejunum (15.6%). These findings suggest that SETDB1-mediated chromatin regulation is pivotal in intestinal IRI and represents a potential therapeutic target

Observation of the Color-Suppressed Decay B̅ 0→D0π0

journal articl

<教育デザインフォーラム学生発表会>理科における資質・能力の育成に向けたメタ認知の高度化を図る実践モデルの検討(ポスター発表の要旨)

departmental bulletin pape

タンパクシツ キノウ ヨソクガク コウザ ガイヨウ

video/mp4講演場所: バイオサイエンス研究科大講義室講演者所属: 奈良先端科学技術大学院大学バイオサイエンス研究科教授vide

Properties of Superplasticized Steel Fiber Reinforced High-Strength Concrete

application/pdfAddition of superplasticizers into plain concrete makes it possible to obtain high slump and high strength at low water-cement ratio. In this study, the similar approach is applied in steel fiber reinforced concrete [SFRC], i.e., it is examined whether SFRC may be useful for improving the small ductility of high strength concrete and whether the superplasticizer may be applicable for overcoming the problems associeted with the low workability of SFRC. This paper describes the results of two series of experiments carried out to obtain fundamental information on the properties of superplasticized steel fiber reinforced concrete with high strength, and to research the effect of superplasticizer admixed with the dosages of 2% or more by weight of cement on the properties of plain concrete.departmental bulletin pape

Lymph nodes 5a: Lymph node yield, 5b: Number of involved nodes by procedure

<p><b>Copyright information:</b></p><p>Taken from "The impact of operative approach on outcome of surgery for gastro-oesophageal tumours"</p><p>http://www.wjso.com/content/5/1/95</p><p>World Journal of Surgical Oncology 2007;5():95-95.</p><p>Published online 20 Aug 2007</p><p>PMCID:PMC2000895.</p><p></p

Kaplan Meier five year survival curves by circumferential resection margin involvement

<p><b>Copyright information:</b></p><p>Taken from "The impact of operative approach on outcome of surgery for gastro-oesophageal tumours"</p><p>http://www.wjso.com/content/5/1/95</p><p>World Journal of Surgical Oncology 2007;5():95-95.</p><p>Published online 20 Aug 2007</p><p>PMCID:PMC2000895.</p><p></p