48 research outputs found
Supplementary Table 1 from Expression of the miR200 Family of microRNAs in Mesothelial Cells Suppresses the Dissemination of Ovarian Cancer Cells
PDF - 11K, Sequence of primers used in the study. Red fonts indicate mutated nucleotides.</p
Supplementary Figure 2 from Expression of the miR200 Family of microRNAs in Mesothelial Cells Suppresses the Dissemination of Ovarian Cancer Cells
PDF - 125K, (A) Mice were intraperitoneally injected with different amounts of recombinant adenovirus, and 2 days later, mice were sacrificed by cervical dislocation. The entire peritoneal cavity was fixed with 2% paraformaldehyde/0.2% glutaraldehyde intraperitoneally for 30 min. X-gal staining was performed, and the peritoneal cavity was opened for visual inspection. (B) X-gal-stained organs and mesothelial cells in the peritoneal cavity are shown.</p
Supplementary Figure 3 from Expression of the miR200 Family of microRNAs in Mesothelial Cells Suppresses the Dissemination of Ovarian Cancer Cells
PDf - 67K, Mice were intraperitoneally injected with rAD-Ctrl or rAD-miR-200 and two days later, ES-2 cells were implanted. Three days later, mice were sacrificed and mesenterium was fixed and stained with anti-FN1 antibody and DAPI.</p
Electrical Properties of Polyethylene Doped with Flame Retardant
application/pdfIn order to improve the electrical properties of flame resistant polyethylene insulating systems, the electric conduction of chlorinated polyethylene(CPE), chlorinated paraffin(CPA), decabromodiphnylether(DBDPE) and their blends with low density polythylene(LDPE) is studied. The electric conductivity σ of CPE decreases as the halogen content ■ increases from 30wt% to 40wt%. This is likely due to a decrease in the mobility of ion because of an increase in internal viscosity. In CPE-LDPE blends, σ sharply increases with increasing ■ and the dielectric relaxation is observed in the ultra low frequency region, being due to an interfacial polarization of CPE and LDPE. These results can be understood by the proposed model where the blend has a two phase structure of CPE and LDPE and a part of CPE forms a conduction path. In CPA-LDPE blends, σ decreases with increasing ■. DBDPE-LDPE blends show a slight increase in σ with increasing ■. The electric conduction of CPA-LDPE and DBDPE-LDPE blends is likely due to the transport of the carrier supplied from CPA and DBDPE through LDPE, respectively. This is supported by an observation of distribution of CPA or DBDPE with an X-ray scanning microanalyser. All the data is replotted against ■ and a comparison is made between various polyethlene insulating systems.departmental bulletin pape
Supplementary Figure 1 from Expression of the miR200 Family of microRNAs in Mesothelial Cells Suppresses the Dissemination of Ovarian Cancer Cells
PDF - 151K, Depletion of FN1 does not affect cancer cell proliferation and promoter activity of MMPs by TGFbeta-stimulated HPMCs. (A) HPMCs were transfected with control or FN1 siRNA, and 24 h later, cells were incubated with or without TGFbeta for 48 h. ES-2 and SKOV3 cells that constitutively expressed luciferase were seeded onto the HPMCs . Cells were lysed 24, 48, and 72 h after cell seeding, and luciferase activity was measured. (B) ES-2 and SKOV3 cells were transfected with pGL4, pGL4-MMP-2/promoter or pGL4-MMP-9/promoter together with pRLTK-luc, and 24 h later, the cells were seeded onto HPMCs treated as in (A). The cells were incubated for 24 h, and then, luciferase activity was measured.</p
Pregnancy-induced ß-cell adaptations in <i>Gcg<sup>gfp/gfp</sup></i> mice.
<p>A, Pancreatic insulin content was measured after a 16-h fasting period (n = 5–7). B, ß-Cell mass was calculated as the relative percentage area of ß-cells (defined as the percentage area of pancreatic sections that stained positive for insulin) multiplied by the weight of the pancreas (n = 3). C, The average number of ki-67-positive ß-cells per ß-cells (n = 3). D, The percentage of serotonin-positive ß-cells per islet (n = 3). Open squares: <i>Gcg<sup>+/+</sup></i> and <i>Gcg<sup>gfp/+</sup></i>; closed squares: <i>Gcg<sup>gfp/gfp</sup></i>. Values are expressed as means±SEM. *<i>p</i><0.05, **<i>p</i><0.01, ***<i>p</i><0.001. E, Immunohistochemical-autofluorescent analyses for GFP (green), insulin (blue) and Ki 67 (red). Islets in non-pregnant <i>Gcg<sup>gfp/+</sup></i> (a), pregnant <i>Gcg<sup>gfp/gfp</sup></i> (b), non-pregnant <i>Gcg<sup>gfp/+</sup></i> (c) and pregnant <i>Gcg<sup>gfp/gfp</sup></i> (d) pancreas. F, Immunohistochemical-autofluorescent analyses for GFP (green), insulin (blue) and serotonin (magenta). Islets in non-pregnant <i>Gcg<sup>gfp/+</sup></i> (a), pregnant <i>Gcg<sup>gfp/gfp</sup></i> (b), non-pregnant <i>Gcg<sup>gfp/+</sup></i> (c) and pregnant <i>Gcg<sup>gfp/gfp</sup></i> (d) pancreas. Scale bars: 100 µm.</p
Fertility, litter size and survival of fetuses/pups in <i>Gcg<sup>gfp/gfp</sup></i> mice.
<p>(A) Pregnancy rate in the control (open squares: <i>Gcg<sup>+/+</sup></i> and <i>Gcg<sup>gfp/+</sup></i>) and <i>Gcg<sup>gfp/gfp</sup></i> (closed squares) mice. Numbers in brackets indicate mating. The rate was not affected by genotype of the male (data not shown). ***p<0.01 by the chi-square test. (B) Litter size in the control and <i>Gcg<sup>gfp/gfp</sup></i> (closed squares) mice at postnatal day 0 and 21. Numbers in brackets indicate litters analyzed. Values are expressed as means±SEM. ***p<0.01 by one-way ANOVA and t-test. (C–E) Survival of fetuses. Female mice were sacrificed at gestational day 9.5 and 15.5. Numbers in brackets indicate number of pregnant females analyzed. Numbers of viable fetuses (C), resorbed fetuses (D) and % resorption (E) are shown. Values are expressed as means±SEM. ***p<0.01 by one-way ANOVA and t-test. (F) Rate of losing litters within 36 hours after delivery. Values are expressed as means±SEM. ***p<0.01 by one-way ANOVA and t-test.</p
