5 research outputs found
<教職大学院>特別支援学校における授業評価・改善の場を「学年」から「学部」へ広げることによる効果の一考察― チームで教育課程を考えるためのしかけのひとつとして ―(報告書の概要)
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CD8α is detected by flow cytometry on CD14monocytes from human peripheral blood
<p><b>Copyright information:</b></p><p>Taken from "CD8α is expressed by human monocytes and enhances FcγR-dependent responses"</p><p>http://www.biomedcentral.com/1471-2172/8/12</p><p>BMC Immunology 2007;8():12-12.</p><p>Published online 1 Aug 2007</p><p>PMCID:PMC2000912.</p><p></p> Anti-CD8α mAb bind monocytes by flow cytometry. , Monocytes were gated by characteristic FSC/SSC scatter (left panel) and expression of high levels of CD14 (right panel) for all flow cytometry studies in this article. , A population of lymphocytes, gated by characteristic FSC/SSC scatter, bind high levels of anti-CD8α. Blood monocytes gated in , bind anti-CD8α mAb. Geometric means of monoclonal antibody binding are shown. Results are representative of five experiments. , Anti-CD8β mAb bind lymphocytes but not monocytes
Subtle differences in CD8α between monocytes and lymphocytes detected by 2-D electrophoresis
<p><b>Copyright information:</b></p><p>Taken from "CD8α is expressed by human monocytes and enhances FcγR-dependent responses"</p><p>http://www.biomedcentral.com/1471-2172/8/12</p><p>BMC Immunology 2007;8():12-12.</p><p>Published online 1 Aug 2007</p><p>PMCID:PMC2000912.</p><p></p> , Western blot with a polyclonal anti-CD8α antibody after 2-D electrophoresis of monocytes and lymphocytes. Cell lysates of lymphocytes and monocytes from one donor were separated by adherence, halved and treated (bottom panels) or not treated (top panels) with neuraminidase before analysis. , Western blot with anti-CD8α mAb D9. , Neuraminidase treatment and western blot with D9 of lymphocyte lysate. Results are representative of experiments with three donors
