37 research outputs found
Studies on substrate specificity of β-xylanase from Streptomyces olivaceoviridis E-86
筑波大学University of Tsukuba博士(農学)Doctor of Philosophy in Agricultural SciencePlant cell wall is the major reservoir of fixed carbon in nature,and consists of three major polymeric components,namely cellulose,hemicellulose,and lignin.β-1,4-Xylans are the most aboundant components of the hemicellulose and mainly found ...1995doctoral thesi
<教職大学院>インクルーシブ教育を推進するためのチームリーダーの在り方についての一考察― 校内研修会を中心とした取組から ―(報告書の概要)
departmental bulletin pape
環境内に埋め込まれた構造に対する情報処理心理学的アプローチの可能性: 事象関連電位を用いた標的出現の予測可能性に関する研究
application/pdfMany previous studies have reported that latencies and/or amplitudes of late components of Event-Related Brain Potentials (ERPs) are influenced by various psychological parameters. In this study the relationship between those components and target predictability was investigated using the Pseudo-Oddball paradigm. Subjects were asked to look at fifteen digits sequentially and to report the total sum after that. Most of the digits were“0”s (I.e. non-targets). Two or three targets (“1” to“9”) were presented according to three types of schedules, which were varied in regularity or predictability. As its regularity decreased, the amplitude of the frontal negative slow wave to the first target increased, whereas there were no effects on the centro-parietal positive components (P3s). These negative components cannot easily be identified with the Contingent Negative Variation in terms of scalp distribution and so on. They seem to be affected by multiple factors such as predictability of the eliciting targets and the following targets.departmental bulletin pape
Repeated BCG treatment of mouse bladder selectively stimulates small GTPases and HLA antigens and inhibits single-spanning uroplakins-2
<p><b>Copyright information:</b></p><p>Taken from "Repeated BCG treatment of mouse bladder selectively stimulates small GTPases and HLA antigens and inhibits single-spanning uroplakins"</p><p>http://www.biomedcentral.com/1471-2407/7/204</p><p>BMC Cancer 2007;7():204-204.</p><p>Published online 2 Nov 2007</p><p>PMCID:PMC2212656.</p><p></p> pyrogen-free saline on days 1, 7, 14, and 21, as described above. Mice were euthanized 24 hours after a single instillation () or 7 days after 4 weekly instillations (and ). Bladders were exposed briefly to formaldehyde for cross-linking of the proteins and DNA together, followed by sonication to fragment the DNA. An antibody against RNA polymerase II (Abcam) was then used to precipitate the DNA transcriptome that was isolated and then purified. The final ChIP DNAs were then used as templates for Q-PCR reactions using primer pairs specific for each gene of interest (Additional File ). Q-PCRs were run in triplicate and the averaged Ct values were transferred into copy numbers of DNA using a standard curve of genomic DNA with known copy numbers. The resulting transcription values for each gene were also normalized for primer pair amplification efficiency using the Q-PCR values obtained with input DNA (un-precipitated genomic DNA). Results are presented as "transcription events detected per 1000 cells" for each gene tested. Error bars correspond to standard deviations from the triplicate Q-PCR reactions. Control represents an untranscribed region of the genome. Asterisks indicate a statistical significant increase (p < 0.05) between BCG-treated and control and a pound sign indicates a statistical significant decrease (p < 0.05) between BCG-treated and control
Comparison of bladder inflammation in response to 10 μM of PAR1-AP in wild type (C57BL/6) and c-kit receptor deficient (Kit/Kit) mice
<p><b>Copyright information:</b></p><p>Taken from "Transcription factor network downstream of protease activated receptors (PARs) modulating mouse bladder inflammation"</p><p>http://www.biomedcentral.com/1471-2172/8/17</p><p>BMC Immunology 2007;8():17-17.</p><p>Published online 17 Aug 2007</p><p>PMCID:PMC2000913.</p><p></p> A = Representative photomicrographs of H&E stained C57BL/6 urinary bladders following PAR1-AP (10 μM)-induced inflammation. PAR1-induced inflammation was characterized in C57BL/6 mice by an intense vasodilation (yellow arrows) and polymorphonuclear leukocyte infiltration (black arrows). B = absence of bladder inflammation in bladders isolated from Kit/Kitmice challenged with PAR1-AP
Target validation of antigen presentation related genes by ChIP/Q-PCR (see figure 3 legend)
<p><b>Copyright information:</b></p><p>Taken from "Repeated BCG treatment of mouse bladder selectively stimulates small GTPases and HLA antigens and inhibits single-spanning uroplakins"</p><p>http://www.biomedcentral.com/1471-2407/7/204</p><p>BMC Cancer 2007;7():204-204.</p><p>Published online 2 Nov 2007</p><p>PMCID:PMC2212656.</p><p></p
ChIP/Q-PCR of uroplakin genes in control and BCG-treated bladder mucosa (see figure 3 legend)
<p><b>Copyright information:</b></p><p>Taken from "Repeated BCG treatment of mouse bladder selectively stimulates small GTPases and HLA antigens and inhibits single-spanning uroplakins"</p><p>http://www.biomedcentral.com/1471-2407/7/204</p><p>BMC Cancer 2007;7():204-204.</p><p>Published online 2 Nov 2007</p><p>PMCID:PMC2212656.</p><p></p
